Strain SIJ_488
(Bacterial strain #68246)

• Purpose: E. coli K-12 MG1655 with genomically integrated arabinose inducible lambda Red recombineering genes, rhamnose inducible flippase recombinase and m-toluic acid inducible I-SceI.
• Depositing Lab: Alex Nielsen
• Publication: Jensen et al Sci Rep. 2015 Dec 8;5:17874. doi: 10.1038/srep17874.

Backbone

• Vector backbone: N/A

Growth in Bacteria

• Bacterial Resistance(s): None
• Growth Temperature: 37°C
• Growth Strain(s): N/A
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: arabinose inducible lambda Red recombineering genes, rhamnose inducible flippase recombinase and m-toluic acid inducible I-SceI.
• Insert Size (bp): 7236

Resource Information

• A portion of this plasmid was derived from a plasmid made by: The genes were inserted into the genome using pSIJ214 (a somewhat instable plasmid, so we do not want to deposit this). This plasmid was made by me.

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

E. coli K-12 MG1655 with genomically integrated arabinose inducible lambda Red recombineering genes, rhamnose inducible flippase recombinase and m-toluic acid inducible I-SceI.