Transcription activator-like effector nuclease (TALEN)-mediated CLYBL targeting enables enhanced transgene expression and one-step generation of dual reporter human induced pluripotent stem cell (iPSC) and neural stem cell (NSC) lines.
Cerbini T, Funahashi R, Luo Y, Liu C, Park K, Rao M, Malik N, Zou J
PLoS One. 2015 Jan 14;10(1):e0116032. doi: 10.1371/journal.pone.0116032. eCollection 2015. PubMed
Cerbini T, Funahashi R, Luo Y, Liu C, Park K, Rao M, Malik N, Zou J
PLoS One. 2015 Jan 14;10(1):e0116032. doi: 10.1371/journal.pone.0116032. eCollection 2015. PubMed
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 62196 | pZT-C13-L1 | Chr.13/CLYBL TALEN expression vector |
| 62197 | pZT-C13-R1 | Chr.13/CLYBL TALEN expression vector |
| 66577 | pAAVS1P-iCAG.copGFP | AAVS1 safe harbor gene targeting donor expressing CAG-driven copGFP, compatible with pZT-AAVS1 TALENs |
| 66578 | pC13N-iCAG.copGFP | CLYBL safe harbor gene targeting donor expressing CAG-driven copGFP, compatible with pZT-C13 TALENs |
| 66579 | pAAVS1P-iCLHN | AAVS1 safe harbor gene targeting donor expressing CAG-driven Nanoluc-HaloTag, compatible with pZT-AAVS1 TALENs |
| 66580 | pC13P-iCLHN | CLYBL safe harbor gene targeting donor expressing CAG-driven Nanoluc-HaloTag, compatible with pZT-C13 TALENs |