PurposeAAVS1 safe harbor gene targeting donor expressing CAG-driven copGFP, compatible with pZT-AAVS1 TALENs
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||66577||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 10001
- Total vector size (bp) 10760
Modifications to backbonecopGFP was cloned under CAG promoter
Vector typeMammalian Expression
Growth in Bacteria
Copy numberHigh Copy
Insert Size (bp)759
- Promoter CAG
- Cloning method Restriction Enzyme
- 5′ cloning site BsrGI (not destroyed)
- 3′ cloning site MluI (not destroyed)
- 5′ sequencing primer gggcggggttcggcttctgg (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pAAVS1P-iCAG.copGFP was a gift from Jizhong Zou (Addgene plasmid # 66577 ; http://n2t.net/addgene:66577 ; RRID:Addgene_66577)
For your References section:Transcription activator-like effector nuclease (TALEN)-mediated CLYBL targeting enables enhanced transgene expression and one-step generation of dual reporter human induced pluripotent stem cell (iPSC) and neural stem cell (NSC) lines. Cerbini T, Funahashi R, Luo Y, Liu C, Park K, Rao M, Malik N, Zou J. PLoS One. 2015 Jan 14;10(1):e0116032. doi: 10.1371/journal.pone.0116032. eCollection 2015. PONE-D-14-41442 [pii] PubMed 25587899