164,410 results

1. PPRE-H2B-eGFP

   Plasmid

   #84393

   Purpose

   Fluorescent reporter for PPARgamma activity

   Depositor

   Severine Degrelle, Thierry Fournier

   Article

   Degrelle et al PPAR Res. 2017;2017:6139107. doi: 10.115

   Insert

   PPARG response element (PPARG Human)
   

   Use

   Tags

   eGFP

   Expression

   Mammalian

   Mutation

   Promoter

   PPRE

   Available since

   Jan. 23, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pBS_12x-601_MA1

   Plasmid

   #114358

   Purpose

   12x Widom 601 nucleosome sequence

   Depositor

   Scot Wolfe

   Article

   Swygert et al Proc Natl Acad Sci U S A. 2018 Dec 4;115

   Insert

   12X601-MA1
   

   Use

   Unspecified

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Feb. 19, 2019

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pCFD5_w

   Plasmid

   #112645

   Purpose

   Ubiquitous expression of one or multiple sgRNAs in Drosophila. Selection marker: Mini-white.

   Depositor

   Michael Boutros

   Insert

   U6:3-tRNA-sgRNA expression cassette
   

   Use

   Tags

   Expression

   Insect

   Mutation

   Promoter

   Available since

   Aug. 13, 2018

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. pDisplay-NE2h-IRES-mCherry-CAAX

   Plasmid

   #208691

   Purpose

   Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2h and a membrane-localized mcherry in mammalian cells

   Depositor

   Yulong Li

   Insert

   GPCR activation based norepinephrine (NE) sensor GRAB_NE2h
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   Oct. 13, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. tdTomato-C1

   Plasmid

   #54653

   Purpose

   Localization: C1 Cloning Vector, Excitation: 554, Emission: 581

   Depositor

   Michael Davidson

   Type

   Empty backbone

   Use

   Tags

   tdTomato

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   June 20, 2014

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

6. TRUPATH Triple Gai1

   Plasmid

   #196048

   Purpose

   Encodes a G alpha subunit (GNAl1) with RLuc8, a G gamma subunit (GNG9) with GFP2 and a G beta subunit (GNB3) as optimal components of a BRET2 biosensor for studying heterotrimeric G proteins

   Depositor

   Justin English

   Article

   Olsen et al Nat Chem Biol. 2020 May 4. pii: 10.1038/

   Inserts

   GAlphai1-RLuc8 (GNAI1 Human)
   GGamma9-GFP2
   GBeta3 (GNB3 Human)
   

   Use

   Luciferase

   Tags

   GFP2 and GSAG linker

   Expression

   Mammalian

   Mutation

   RLuc8 and flanking SGGGGS linkers have been inser…

   Promoter

   CMV

   Available since

   March 15, 2023

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. pAGK62

   Plasmid

   #228122

   Purpose

   Express node 1531 retron with split retron ncRNA and gRNA to edit (10bp barcode) EMX1 gene in human cells

   Depositor

   Seth Shipman

   Article

   Khan et al Nat Biotechnol. 2024 Sep 17. doi: 10.103

   Insert

   Retron node 1531 split retron ncRNA and gRNA to edit EMX1 gene (10bp barcode) in human cells
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG for RT, U6/H1 for ncRNA/gRNA

   Available since

   Dec. 5, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. UKNP1.16.3

   Plasmid

   #98188

   Purpose

   Expression of HCV E1E2 genes cloned from patient serum. United Kingdom Nottingham Panel x.xx.xx (genotype.patient number.clone number)

   Depositor

   Jonathan Ball

   Article

   Urbanowicz et al J Virol. 2015 Dec 23;90(7):3288-301. doi

   Insert

   HCV E1E2
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Aug. 2, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. pET28-FnCas12a-TEV

   Plasmid

   #174658

   Purpose

   FnCas12a expression plasmid

   Depositor

   Jin Wang, Huimin Zhao

   Article

   Enghiad et al Nat Commun. 2021 Feb 19;12(1):1171. doi:

   Insert

   FnCas12a
   

   Use

   CRISPR

   Tags

   Expression

   Bacterial

   Mutation

   Promoter

   Available since

   Oct. 4, 2021

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pBMN YFP-FKBP(DD)

    Plasmid

    #31766

    Depositor

    Thomas Wandless

    Article

    Chu et al Bioorg Med Chem Lett. 2008 Nov 15;18(22)

    Insert

    HA-YFP-FKBP(DD) (FKBP1A Human)
    

    Use

    Retroviral

    Tags

    HA and YFP

    Expression

    Mutation

    E31G, F36V, R71G and K105E on FKBP12

    Promoter

    LTR

    Available since

    Oct. 4, 2011

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. TFORF1665

    Plasmid

    #141863

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    TP73 (TP73 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. TAp63alpha-FLAG

    Plasmid

    #27008

    Depositor

    David Sidransky

    Insert

    TAp63alpha (TP63 Human)
    

    Use

    Tags

    FLAG

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Dec. 15, 2010

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. pET28a(+) NRP1 b1 (273-427)

    Plasmid

    #162734

    Purpose

    Bacterial expression of the b1 domain from the human Neuropilin 1 receptor (NRP1). With an N-terminal 6His tag and thrombin cleavage site.

    Depositor

    Brett Collins

    Article

    Daly et al Science. 2020 Oct 20. pii: science.abd30

    Insert

    Human NRP1 b1 domain residues 273-427
    

    Use

    Tags

    Expression

    Bacterial

    Mutation

    Codon optimised

    Promoter

    Available since

    April 27, 2021

    Availability

    Industry, Academic Institutions, and Nonprofits

    

    Enlarge

14. pAAV-nEF-Coff/Fon-ChR2(ET/TC)-EYFP (AAV8)

    Viral Prep

    #137141-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-nEF-Coff/Fon-ChR2(ET/TC)-EYFP (#137141). In addition to the viral particles, you will also receive purified pAAV-nEF-Coff/Fon-ChR2(ET/TC)-EYFP plasmid DNA. nEF-driven, Flp-dependent expression of ChR2(E123T/T159C)-EYFP (inhibited in presence of Cre) for optogenetic activation. These AAV preparations are suitable purity for injection into animals.

    Depositor

    INTRSECT 2.0 Project, Karl Deisseroth

    Article

    Fenno et al Neuron. 2020 Jun 20. pii: S0896-6273(20)

    Promoter

    nEF

    Tags

    EYFP (Flp-dependent)

    Available since

    May 6, 2021

    Availability

    Academic Institutions and Nonprofits only

15. pBABE puro human IRS-1

    Plasmid

    #11025

    Depositor

    Ronald Kahn

    Article

    Bruning et al Mol Cell Biol. 1997 Mar . 17(3):1513-21.

    Insert

    IRS-1 (IRS1 Human)
    

    Use

    Retroviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    May 24, 2006

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. pCDNA3 IRF9-STAT2C

    Plasmid

    #37544

    Depositor

    Curt Horvath

    Article

    Kraus et al J Biol Chem. 2003 Apr 11;278(15):13033-8

    Insert

    IRF-9 STAT2 C (IRF9, STAT2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    March 17, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pEGFP-N1_hTRIM32

    Plasmid

    #69541

    Purpose

    Expresses human TRIM32 fused to c-terminal EGFP

    Depositor

    Martin Dorf

    Article

    Fu et al PLoS Pathog. 2015 Jun 9;11(6):e1004960.

    Insert

    tripartite motif containing 32 (TRIM32 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 19, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pDONR223_BTK_WT

    Plasmid

    #82197

    Purpose

    Gateway Donor vector containing BTK , part of the Target Accelerator Plasmid Collection.

    Depositor

    David Root, Jesse Boehm, William Hahn

    Article

    Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

    Insert

    BTK (BTK Human)
    

    Use

    Gateway entry vector

    Tags

    Expression

    Mutation

    WT

    Promoter

    None

    Available since

    April 24, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pMSCV-Cas9-2A-GFP-sgRNA

    Plasmid

    #124889

    Purpose

    Contains spCas9-2A-GFP and guide RNA scaffold in single retroviral vector

    Depositor

    Harvey Lodish

    Article

    Li et al Haematologica. 2016 Jun;101(6):e216-9. d

    Insert

    Cas9
    

    Use

    Retroviral

    Tags

    2A-GFP

    Expression

    Mutation

    Promoter

    MSCV

    Available since

    May 3, 2019

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. Flag-ZDHHC20

    Plasmid

    #231668

    Purpose

    Expresses human ZDHHC20 protein

    Depositor

    Chunhong Ma

    Article

    Zhang et al Sci Immunol. 2024 Nov 15;9(101):eadp7302

    Insert

    ZDHHC20 (ZDHHC20 Human)
    

    Use

    Tags

    3×Flag Tag

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Feb. 27, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge