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168,548 results

Showing: 5101 - 5120 of 168548 results

  1. pCAG-CBE4max-SpCas9-NG-P2A-EGFP (RTW4554)

    Plasmid
    #140001
    Purpose
    CAG promoter expression plasmid for human codon optimized BE4max C-to-T base editor with SpCas9-NG(D10A/L1111R/D1135V/G1218R/E1219F/A1322R/R1335V/T1337R) and P2A-EGFP
    Depositor
    Benjamin Kleinstiver
    Article
    Walton et al Science. 2020 Apr 17;368(6488):290-296.
    Insert
    human codon optimized CBE4max SpCas9-NG with P2A-EGFP
    Use
    CRISPR
    Tags
    P2A-EGFP
    Expression
    Mammalian
    Mutation
    nSpCas9=D10A; SpCas9-NG=L1111R/D1135V/G1218R/E121…
    Promoter
    CAG
    Available Since
    March 26, 2020
    Availability
    Academic Institutions and Nonprofits only
    140001-plasmid-map-sequence-id-477414

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  5. pAAVS1_TREtight-GEM2-Halo-FRB-IRES-FKBP-SNAP-GFPNb_EF1a-rtTA3-T2A-Puro-P2A-Thy1.1

    Plasmid
    #197061
    Purpose
    Donor plasmid for human AAVS1 locus knock-in, doxycycline-inducible expression of GEM2-Halo-FRB and Adaptor Protein (FKBP-SNAP-vhhGFP4)
    Depositor
    Julia Mahamid
    Article
    Fung et al Nat Methods. 2023 Dec;20(12):1900-1908.
    Inserts
    Encapsulin
    Adaptor Protein
    Use
    CRISPR
    Tags
    FRB and HaloTag
    Expression
    Mammalian
    Mutation
    Codon-optimised for human cell expression
    Available Since
    Sept. 20, 2023
    Availability
    Academic Institutions and Nonprofits only
    197061-plasmid-map-sequence-id-402602

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  11. pCMV5-25BoxB

    Plasmid
    #60817
    Purpose
    Cloning vector, 25 repeats of 19 bp BoxB sequence from bacteriophage-λ, for RNA labeling. BoxB-RNA is bound by 22 amino acid RNA binding domain of λ-anti-terminator protein N, e.g. tagged with GFP
    Depositor
    Maria Carmo-Fonseca
    Article
    Martin et al Cell Rep. 2013 Sep 26;4(6):1144-55. doi:
    Insert
    25x BoxB
    Use
    Synthetic Biology
    Expression
    Mammalian
    Mutation
    25 repeats of BoxB sequence
    Promoter
    CMV
    Available Since
    Nov. 16, 2014
    Availability
    Academic Institutions and Nonprofits only
    60817-plasmid-map-sequence-id-199089

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  12. pIVM_02

    Plasmid
    #204500
    Purpose
    Expression of VHL (54-213) for production of VCB complexes in conjunction with plasmid pIVM_26 (EloB 1-104, EloC 17-112)
    Depositor
    Alessio Ciulli
    Article
    Cardote et al Structure. 2017 Jun 6;25(6):901-911.e3.
    Insert
    amino acids 54-213 VHL (VHL Human)
    Tags
    6xHis, TEV, and Thrombin
    Expression
    Bacterial
    Mutation
    0
    Available Since
    Sept. 5, 2023
    Availability
    Academic Institutions and Nonprofits only
    204500-plasmid-map-sequence-id-406419

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  15. IDG_TMEM63A_OE_1

    Plasmid
    #161667
    Purpose
    Overexpresses TMEM63A
    Depositor
    Mike McManus
    Insert
    TMEM63A (TMEM63A Human)
    Tags
    V5
    Expression
    Mammalian
    Available Since
    Dec. 18, 2021
    Availability
    Academic Institutions and Nonprofits only
    161667-plasmid-map-sequence-id-319659

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  16. pAAV-S5E2-GCaMP6f (AAV1)

    Viral Prep
    #135632-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-S5E2-GCaMP6f (#135632). In addition to the viral particles, you will also receive purified pAAV-S5E2-GCaMP6f plasmid DNA. Expression of GCaMP6f under the control of the E2 regulatory element. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Jordane Dimidschstein
    Article
    Vormstein-Schneider et al Nat Neurosci. 2020 Dec;23(12):1629-1636.
    Available Since
    Nov. 11, 2020
    Availability
    Academic Institutions and Nonprofits only

  17. pMycoFos

    Plasmid
    #84577
    Purpose
    Shuttle and expression vector. Replicates in E.coli and Mycobacterium. Gives acetamide-inducible gene expression in Mycobacterium. Copy number is controllable by arabinose in E.coli.
    Depositor
    Nicholas Coleman
    Article
    Ly et al J Microbiol Methods. 2011 Sep;86(3):320-
    Type
    Empty backbone
    Expression
    Bacterial
    Available Since
    April 7, 2017
    Availability
    Industry, Academic Institutions, and Nonprofits
    84577-plasmid-map-sequence-id-175425

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  19. pLSC-5

    Plasmid
    #62889
    Purpose
    Lenti vector for expression of inducible split Cas9
    Depositor
    Feng Zhang
    Article
    Zetsche et al Nat Biotechnol. 2015 Feb 2. doi: 10.1038
    Inserts
    SpCas9(574-1368)
    SpCas9(2-573)
    Use
    CRISPR and Lentiviral
    Tags
    FFBP12, FRB, NES PTK2, NLS SV40, and P2A
    Expression
    Mammalian
    Mutation
    *see comment below
    Promoter
    EFS
    Available Since
    March 26, 2015
    Availability
    Academic Institutions and Nonprofits only
    62889-plasmid-map-sequence-id-105912

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  20. pENN.AAV.CamKII0.4.eGFP.WPRE.rBG

    Plasmid
    #105541
    Purpose
    AAV expression of EGFP from CamKII promoter
    Depositor
    James M. Wilson
    Has Service
    AAV1 and AAV5
    Insert
    EGFP
    Use
    AAV
    Expression
    Mammalian
    Promoter
    CamkII
    Available Since
    Feb. 2, 2018
    Availability
    Academic Institutions and Nonprofits only
    105541-plasmid-map-sequence-id-202384

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Showing: 5101 - 5120 of 168548 results