|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||104162||Standard format: Plasmid sent in bacteria as agar stab||1||$65|
This material is available to academics and nonprofits only.
Vector backbonepQE80 derivative
- Total vector size (bp) 4673
Vector typeBacterial Expression
Growth in Bacteria
Copy numberHigh Copy
Gene/Insert nameAnti-mouse kappa chain specific nanobody TP1170 (1xCysteine)
/ Fusion Proteins
- 14xHistidine tag (N terminal on backbone)
- NEDD8 from Brachypodium distachyon (cleavable by NEDP1) (N terminal on backbone)
Terms and Licenses
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pTP1174 was a gift from Dirk Görlich (Addgene plasmid # 104162 ; http://n2t.net/addgene:104162 ; RRID:Addgene_104162)
For your References section:A toolbox of anti–mouse and anti–rabbit IgG secondary nanobodies. Pleiner T, Bates M, Görlich D. J Cell Biol Dec 2017, jcb.201709115 10.1083/jcb.201709115