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CRISPR-Sirius: RNA scaffolds for signal amplification in genome imaging.

Ma H, Tu LC, Naseri A, Chung YC, Grunwald D, Zhang S, Pederson T
Nat Methods. 2018 Nov;15(11):928-931. doi: 10.1038/s41592-018-0174-0. Epub 2018 Oct 30. (Link opens in a new window) PubMed

Plasmids from Article

ID Plasmid Purpose
121936pHAGE-TO-dCas9-P2A-HSACRISPR-Sirius plasmid
121937pHAGE-EFS-MCP-HALOnlsCRISPR-Sirius plasmid
121938pHAGE-EFS-PCP-GFPnlsCRISPR-Sirius plasmid
121939pLH-sgRNA-Sirius-8XMS2CRISPR-Sirius plasmid
121940pLH-sgRNA-Sirius-8XPP7CRISPR-Sirius plasmid
121941pLH-sgRNA-Sirius-4X(MS2-PP7)CRISPR-Sirius plasmid
121942pPUR-hU6-sgRNA-Sirius-8XMS2CRISPR-Sirius plasmid
121943pPUR-mU6-sgRNA-Sirius-8XPP7CRISPR-Sirius plasmid
121944pPUR-hU6-Sirius-8XMS2-mU6-Sirius-8XPP7CRISPR-Sirius plasmid

Antibodies from Article