pFUGW-hGtACR2-EYFP
(Plasmid #67877)

• Purpose: Mammalian expression construct of human codon-adapted anion channelrhodopsin 2 from Guillardia theta fused in frame to EYFP via a NotI site in the pFUGW vector backbone
• Depositing Lab: John Spudich
• Publication: Govorunova et al Science. 2015 Jun 25. pii: aaa7484.

Backbone

• Vector backbone: FUGW
• Vector type: Mammalian Expression, Lentiviral

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: hGtACR2
• Alt name: GtACR2
• Alt name: Guillardia theta anion channelrhodopsin (ACR)
• Species: Guillardia theta
• Insert Size (bp): 1601
• Promoter: UbC
• Tag / Fusion Protein:
  • EYFP (C terminal on insert)

Cloning Information

• Cloning method: Unknown
• 5′ sequencing primer: n/a

Resource Information

• Addgene Notes:
  • Addgene Diagnostic Digest
• Articles Citing this Plasmid:
  • 8 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry

Depositor Comments

Mammalian Expression construct of human codon-adapted anion channelrhodopsin 2 from Guillardia theta fused in frame to EYFP via a NotI site, in the pFUGW vector backbone