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secretedBirA-8his
(Plasmid #32408)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 32408 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pTT3
  • Backbone manufacturer
    Yves Durocher
  • Backbone size w/o insert (bp) 6000
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    BirA
  • Alt name
    Biotin ligase
  • Species
    E. coli
  • Insert Size (bp)
    1121
  • Tag / Fusion Protein
    • 8 His tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (destroyed during cloning)
  • 3′ cloning site Xba1 (not destroyed)
  • 5′ sequencing primer CMV Forward
  • (Common Sequencing Primers)

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pTT3 was obtained from Yves Durocher, National Research Council of Canada-Biotechnology Research Institute
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    secretedBirA-8his was a gift from Gavin Wright (Addgene plasmid # 32408 ; http://n2t.net/addgene:32408 ; RRID:Addgene_32408)
  • For your References section:

    Large-scale screening for novel low-affinity extracellular protein interactions. Bushell KM, Sollner C, Schuster-Boeckler B, Bateman A, Wright GJ. Genome Res. 2008 Apr;18(4):622-30. Epub 2008 Feb 22. 10.1101/gr.7187808 PubMed 18296487