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secreted BirA-Flag
(Plasmid #64395)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 64395 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pTT3
  • Backbone manufacturer
    Yves Durocher
  • Backbone size w/o insert (bp) 6000
  • Total vector size (bp) 7043
  • Vector type
    Mammalian Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    BirA
  • Alt name
    Biotin ligase
  • Species
    E. coli
  • Insert Size (bp)
    1000
  • Entrez Gene
    birA (a.k.a. b3973, ECK3965, JW3941, bioR, dhbB)
  • Promoter CMV
  • Tag / Fusion Protein
    • Flag tag (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site Sal1 (destroyed during cloning)
  • 3′ cloning site Xba1 (not destroyed)
  • 5′ sequencing primer CMV forward
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    secreted BirA-Flag was a gift from Gavin Wright (Addgene plasmid # 64395)
  • For your References section:

    Large-scale screening for novel low-affinity extracellular protein interactions. Bushell KM, Sollner C, Schuster-Boeckler B, Bateman A, Wright GJ. Genome Res. 2008 Apr;18(4):622-30. Epub 2008 Feb 22. 10.1101/gr.7187808 PubMed 18296487