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CD4d3+4-blac
(Plasmid #32403)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 32403 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEMCS
  • Backbone manufacturer
    Mark Bushell
  • Backbone size (bp) 6000
  • Vector type
    Mammalian Expression
  • Promoter CMV promoter
  • Tags / Fusion Proteins
    • ratCd4d3+4 (C terminal on insert)
    • pentamerization domain of rat cartilaginous oligomeric matrix protein (COMP) (C terminal on insert)
    • beta-lactamase (C terminal on insert)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    Unknown

Resource Information

  • A portion of this plasmid was derived from a plasmid made by
    pTT3 was obtained from Yves Durocher, National Research Council of Canada-Biotechnology Research Institute
  • Terms and Licenses

Depositor Comments

Please note that Addgene's sequencing result with the rCD4-F primer found a single nucleotide mismatch at bp# 858, when compared to the full plasmid sequence provided by the depositing scientist. According to the depositing scientist, this is a silent mutation in the COMP domain of the protein and should not affect protein expression.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    CD4d3+4-blac was a gift from Gavin Wright (Addgene plasmid # 32403)
  • For your References section:

    Large-scale screening for novel low-affinity extracellular protein interactions. Bushell KM, Sollner C, Schuster-Boeckler B, Bateman A, Wright GJ. Genome Res. 2008 Apr;18(4):622-30. Epub 2008 Feb 22. 10.1101/gr.7187808 PubMed 18296487