PurposeA positive control receptor bait (rat CD4d3+4-bio) and prey (rat CD200) EXPRESs plasmid for The Basic AVEXIS kit
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||32404||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
Backbone manufacturerMark Bushell
- Backbone size w/o insert (bp) 6000
Vector typeMammalian Expression
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
SpeciesR. norvegicus (rat)
Insert Size (bp)714
- Promoter CMV
/ Fusion Proteins
- Cd4d3+4 (C terminal on insert)
- biotinylation peptide (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site NotI (not destroyed)
- 3′ cloning site AscI (not destroyed)
- 5′ sequencing primer CMV Forward (Common Sequencing Primers)
A portion of this plasmid was derived from a plasmid made bypTT3 was obtained from Yves Durocher, National Research Council of Canada-Biotechnology Research Institute
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
Please note that Addgene's sequencing result with the LNCX primer found a single nucleotide mismatch at bp# 6587 when compared to the full plasmid sequence provided by the depositing scientist. According to the depositing scientist, the mismatch is in the Kozak sequence preceding the CD200bio open reading frame and should not affect protein expression.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:CD200Cd4d3+4-bio was a gift from Gavin Wright (Addgene plasmid # 32404 ; http://n2t.net/addgene:32404 ; RRID:Addgene_32404)
For your References section:Large-scale screening for novel low-affinity extracellular protein interactions. Bushell KM, Sollner C, Schuster-Boeckler B, Bateman A, Wright GJ. Genome Res. 2008 Apr;18(4):622-30. Epub 2008 Feb 22. 10.1101/gr.7187808 PubMed 18296487