DCP1 forms asymmetric trimers to assemble into active mRNA decapping complexes in metazoa.
Tritschler F, Braun JE, Motz C, Igreja C, Haas G, Truffault V, Izaurralde E, Weichenrieder O
Proc Natl Acad Sci U S A. 2009 Dec 22. 106(51):21591-6. PubMed Article
Tritschler F, Braun JE, Motz C, Igreja C, Haas G, Truffault V, Izaurralde E, Weichenrieder O
Proc Natl Acad Sci U S A. 2009 Dec 22. 106(51):21591-6. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 25030 | pT7-EGFP-C1-HsDCP1a | |
| 25031 | pT7-EGFP-C1-HsDCP2 | |
| 25032 | pT7-EGFP-C1-HsEDC3 | |
| 25033 | pT7-EGFP-C1-HsRCK | |
| 66597 | pCIneo-lambdaN-HA-HsEDC4 | Expresses lambdaN-HA-tagged EDC4 in mammalian cells |
| 146400 | pCIneo-lambdaN-HA-HsDCP1a_G | Mammalian Expression of HsDcp1a |
| 146401 | pCIneo-lambdaN-HA-HsDCP2_G | Mammalian Expression of HsDCP2 |
| 146402 | pRSFDuetG-DmDCP1_328-366 (MCSII)_G | Bacterial Expression of DmDCP1_328-366 |
| 146403 | pRSFDuetG-DmDCP1_328-366 (MCSII)-mut1_G | Bacterial Expression of DmDCP1_328-366-mut1 |
| 146404 | pRSFDuetG-DmDCP1_328-366 (MCSII)-mut2_G | Bacterial Expression of DmDCP1_328-366-mut2 |
| 146405 | pRSFDuetG-DmDCP1_328-366 (MCSII)-mut3_G | Bacterial Expression of DmDCP1_328-366-mut3 |
| 146406 | pRSFDuetG-DmDCP1_328-366 (MCSII)-WT_G | Bacterial Expression of DmDCP1_328-366 |
| 146407 | pRSFDuetG-HsDCP1a_539-582 (MCSII)_G | Bacterial Expression of HsDCP1a_539-582 |
| 146408 | pRSFDuetG-HsDCP1a_539-582 (MCSII)-mut1_G | Bacterial Expression of HsDCP1a_539-582-mut1 |
| 146409 | pRSFDuetG-HsDCP1a_539-582 (MCSII)-mut2_G | Bacterial Expression of HsDCP1a_539-582-mut2 |
| 146410 | pRSFDuetG-HsDCP1a_539-582 (MCSII)-mut3_G | Bacterial Expression of HsDCP1a_539-582-mut3 |
| 146411 | pRSFDuetG-HsDCP1a_539-582 (MCSII)-WT_G | Bacterial Expression of HsDCP1a_539-582 |
| 146412 | pT7-EGFP-C1-HsDCP1a_539-582_G | Mammalian Expression of HsDcp1a_539-582 |
| 146413 | pT7-EGFP-C1-HsDCP1a-del155-168_G | Mammalian Expression of HsDcp1a-del155-168 |
| 146414 | pT7-EGFP-C1-HsDCP1a-del539-582_G | Mammalian Expression of HsDcp1a-del539-582 |
| 146415 | pT7-EGFP-C1-HsDCP1a-mut1_G | Mammalian Expression of HsDCP1a-mut1 |
| 146416 | pT7-EGFP-C1-HsDCP1a-mut2_G | Mammalian Expression of HsDCP1a-mut2 |
| 146417 | pT7-EGFP-C1-HsDCP1a-mut3_G | Mammalian Expression of HsDCP1a-mut3 |
| 146500 | pAc5.1B-EGFP-DmDCP1_327-372_H | Insect Expression of DmDCP1_327-372 |
| 146501 | pAc5.1B-HA-DmDCP1_1-327-dsRNAres_H | Insect Expression of DmDCP1_1-327 |
| 146502 | pAc5.1B-HA-DmDCP1-dsRNAres_H | Insect Expression of DmDCP1 |
| 146503 | pAc5.1B-lambdaN-HA-DmDCP1_327-372-GST-V5His6_H | Insect Expression of DmDCP1_327-372 |
| 146504 | pCIneo-lambdaN-HA-HsDCP1a-withDmTD_H | Mammalian Expression of HsDcp1a-withDmTD |
| 146505 | pLNHA-C1-HsDCP1b_H | Mammalian Expression of HsDCP1b |
| 146506 | pT7-EGFP-C1-HsDCP1a-withDmTD_H | Mammalian Expression of HsDCP1a-withDmTD |