Targeting of heparanase-modified syndecan-1 by prosecretory mitogen lacritin requires conserved core GAGAL plus heparan and chondroitin sulfate as a novel hybrid binding site that enhances selectivity.
Zhang Y, Wang N, Raab RW, McKown RL, Irwin JA, Kwon I, van Kuppevelt TH, Laurie GW
J Biol Chem. 2013 Apr 26;288(17):12090-101. doi: 10.1074/jbc.M112.422717. Epub 2013 Mar 15. PubMed Article
Zhang Y, Wang N, Raab RW, McKown RL, Irwin JA, Kwon I, van Kuppevelt TH, Laurie GW
J Biol Chem. 2013 Apr 26;288(17):12090-101. doi: 10.1074/jbc.M112.422717. Epub 2013 Mar 15. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 52326 | pTT5-hSDC1 | expresses human syndecan 1 in HEK293-EBNA1 (293E) suspension culture. |
| 52355 | pTT5-hSDC1-N20 | expresses human syndecan 1 'del 1- 20' truncation mutant in HEK293-EBNA1 (293E) suspension culture. |
| 52363 | pTT5-hSDC1-S15A | expresses human Syndecan-1 serine 15 mutated to alanine (numbering excludes signal peptide) in HEK293-EBNA1 (293E) suspension culture. |
| 52364 | pTT5-hSDC1-S15/25A | expresses human Syndecan-1 serine 15 and 25 mutated to alanine (numbering excludes signal peptide) in HEK293-EBNA1 (293E) suspension culture. |
| 52365 | pTT5-hSDC1-CS1 | expresses human Syndecan-1 point mutant at S184A in HEK293-EBNA1 (293E) suspension culture. |
| 52367 | pTT5-hSDC1-CS2 | expresses human Syndecan-1 point mutant at S194A in HEK293-EBNA1 (293E) suspension culture. |
| 52370 | pTT5-hSDC1-CS1/CS2 | expresses human Syndecan-1 point mutant at S184A and S194A in HEK293-EBNA1 (293E) suspension culture. |
| 52375 | pTT5-hSDC1-4 | expresses human Syndecan-1 GAGAL replaced with GDLDD of SDC4 in HEK293-EBNA1 (293E) suspension culture. |
| 52378 | pTT5-hSDC1-2 | expresses human Syndecan-1 GAGAL replaced with GADEV of SDC2 in HEK293-EBNA1 (293E) suspension culture. |