165,888 results

1. pET StrepII-StrepII TEV LIC cloning vector (2RRT)

   Plasmid

   #55215

   Depositor

   Scott Gradia

   Type

   Empty backbone

   Use

   Tags

   2xStrep II and TEV cleavage site

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available Since

   June 16, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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2. pFLAG-CMV-hErk1

   Plasmid

   #49328

   Purpose

   Expresses FLAG-tagged human Erk1

   Depositor

   Melanie Cobb

   Insert

   Erk1 (MAPK3 Human)
   

   Use

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Jan. 7, 2014

   Availability

   Academic Institutions and Nonprofits only

   

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3. pAAV-hSynapsin1-axon-jGCaMP8s-P2A-mRuby3 (AAV1)

   Viral Prep

   #172921-AAV1

   Purpose

   Ready-to-use AAV1 particles produced from pAAV-hSynapsin1-axon-jGCaMP8s-P2A-mRuby3 (#172921). In addition to the viral particles, you will also receive purified pAAV-hSynapsin1-axon-jGCaMP8s-P2A-mRuby3 plasmid DNA. hSyn-driven expression of calcium sensor jGCaMP8s in axons with cytosolic expression of mRuby3. These AAV preparations are suitable purity for injection into animals.

   Depositor

   GENIE Project

   Promoter

   Syn

   Tags

   mRuby3

   Available Since

   Aug. 24, 2022

   Availability

   Academic Institutions and Nonprofits only

4. pLV[Exp]-Puro-CMV>{5-HT6-R_GECO1.1}

   Plasmid

   #232879

   Purpose

   Expresses the 5-HT6R coupled to the RGECO1 calcium sensor

   Depositor

   Paul DeCaen

   Article

   DeCaen et al J Cell Physiol. 2025 Apr;240(4):e70034.

   Insert

   5-Hydroxytryptamine Receptor 6 (HTR6 Human)
   

   Use

   Lentiviral

   Tags

   RGECO1.1

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   April 17, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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5. pECFP_C1_NSD2 FL

   Plasmid

   #221382

   Purpose

   Expresses NSD2 in mammalian cells

   Depositor

   Albert Jeltsch

   Article

   Weirich et al Commun Biol. 2024 Jun 8;7(1):707. doi: 1

   Insert

   Histone-lysine N-methyltransferase NSD2 (NSD2 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   L237R mutation in ECFP (Please see depositor comm…

   Promoter

   CMV

   Available Since

   June 21, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCMV-PEmax-SpG

   Plasmid

   #211549

   Purpose

   Prime editing in mammalian cells

   Depositor

   Yongsub Kim

   Article

   Jang et al Commun Biol. 2023 Jun 30;6(1):681. doi:

   Insert

   PEmax-SpG
   

   Use

   CRISPR

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Dec. 21, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. pRK5-HA-Ubiquitin-K33

   Plasmid

   #17607

   Purpose

   Mammalian expression of HA tagged ubiquitin with K33 only, other lysines mutated to arginines

   Depositor

   Ted Dawson

   Article

   Lim et al J Neurosci. 2005 Feb 23. 25(8):2002-9.

   Insert

   Ubiquitin C (UBC Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   K33 only, other lysines mutated to arginines; P19…

   Promoter

   Available Since

   March 31, 2008

   Availability

   Academic Institutions and Nonprofits only

   

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8. pLV-CMV-KIF2C

   Plasmid

   #205994

   Purpose

   Over-expression of GFP-KIF2c

   Depositor

   Floris Foijer

   Article

   Hong et al Nature. 2022 Jul;607(7918):366-373. doi:

   Insert

   KIF2c (KIF2C Human)
   

   Use

   Tags

   GFP

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available Since

   Nov. 20, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. CMVTO-Furin

   Plasmid

   #179920

   Purpose

   Furin protein

   Depositor

   Xiaojing Gao

   Article

   Vlahos et al Nat Commun. 2022 Feb 17;13(1):912. doi:

   Insert

   Furin
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available Since

   March 23, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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10. Human Brunello kinome pooled library, guides 1-8 in lentiCRISPRv2 backbone

    Pooled Library

    #1000000083

    Purpose

    CRISPR gRNA knockout pooled library for targeting the human kinome

    Depositor

    John Doench, David Root

    Available Since

    June 27, 2016

    Availability

    Academic Institutions and Nonprofits only

11. pcDNA3.1_T6B -mCherry

    Plasmid

    #205600

    Purpose

    expresses human T6B peptide fused with mChery in mammalian cells

    Depositor

    Marc Friedlander

    Article

    Sekar et al Nat Biotechnol. 2023 Sep 21. doi: 10.103

    Insert

    T6B peptide (fragment of human TRNC6B 599-683aa) (TNRC6B Human)
    

    Use

    Tags

    mCherry

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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12. TMPRSS2

    Plasmid

    #53887

    Purpose

    Expression of TMPRSS2

    Depositor

    Roger Reeves

    Article

    Edie et al G3 (Bethesda). 2018 Jul 2;8(7):2215-2223

    Has Service

    Cloning Grade DNA

    Insert

    TMPRSS2 (TMPRSS2 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    SP6 and CMV

    Available Since

    March 27, 2018

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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13. pMRS-XylS-dCas9

    Plasmid

    #220196

    Purpose

    mini-Tn7 dCas9 expression vector - XylS/pM promoter

    Depositor

    Larry Halverson

    Article

    Roghair Stroud et al ACS Synth Biol. 2024 Aug 20. doi: 10.102

    Insert

    Pseudomonas aeruginosa-codon optimized Streptococcus pyogenes dCas9
    

    Use

    CRISPR

    Tags

    Expression

    Bacterial

    Mutation

    Pseudomonas aeruginosa codon optimized

    Promoter

    XylS/pM

    Available Since

    Sept. 25, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. p11-LacY-wtx1

    Plasmid

    #69056

    Purpose

    The reporter plasmid p11-LacY-wtx1 encodes the toxin CcdB gene under the inducible BAD promoter and a single copy of the endonuclease cleavage site which is readily extendable to multiple copies.

    Depositor

    Huimin Zhao

    Article

    Chen et al Nucleic Acids Res. 2005 Oct 6;33(18):e15

    Inserts

    ccdB (ccdB )
    lacY (lacY )
    

    Use

    Tags

    A177C

    Expression

    Mutation

    Promoter

    pBAD and pLac

    Available Since

    Sept. 30, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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15. sgTP53_3

    Plasmid

    #78164

    Purpose

    sgTP53

    Depositor

    William Hahn

    Article

    Hong et al Nat Commun. 2016 Jun 22;7:11987. doi: 10

    Insert

    TP53 (TP53 Human)
    

    Use

    CRISPR and Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    hU6

    Available Since

    June 22, 2016

    Availability

    Academic Institutions and Nonprofits only

16. pHN922

    Plasmid

    #178975

    Purpose

    Expression of GST-His8-GFP-nanobody

    Depositor

    Kazuhiro Abe

    Insert

    GST-His8-GFP-nanobody
    

    Use

    Affinity Reagent/ Antibody

    Tags

    GST, His8

    Expression

    Bacterial

    Mutation

    Promoter

    tac

    Available Since

    Feb. 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. AAV:ITR-EF1aCore-SaCas9-dual(U6-sgRNA(backbone))-ITR

    Plasmid

    #207878

    Purpose

    AAV vector encoding EF1a core promoter-driven SaCas9 and two sgRNA cloning sites w/ the engineered Sa Guide scaffold variant (BbsI and PaqCI for sgRNA cloning).

    Depositor

    Yeh-Hsing Lao

    Article

    Lao et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    Oct. 13, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. pHAGE TRE dCas9-KRAB

    Plasmid

    #50917

    Purpose

    Tet-regulatable dCas9-KRAB 2nd generation lentiviral expression vector

    Depositor

    Rene Maehr, Scot Wolfe

    Article

    Kearns et al Development. 2014 Jan;141(1):219-23. doi

    Insert

    dCas9
    

    Use

    CRISPR and Lentiviral

    Tags

    3xHA and KRAB

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically inactive)

    Promoter

    TRE

    Available Since

    Feb. 11, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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19. CP1041-ABE8e

    Plasmid

    #138493

    Purpose

    A-to-G base editor

    Depositor

    David Liu

    Article

    Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

    Insert

    ecTadA(8e)-nSpCas9 (CP1041)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available Since

    March 16, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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20. alpha7nACHR_Nacho_GCAMP7s

    Plasmid

    #182940

    Purpose

    alpha7 nACHR expression in mammalian cell lines with calcium reporter

    Depositor

    Robert Brenner

    Article

    Belanger-Coast et al Eur J Pharmacol. 2022 Jun 15;925:175000.

    Insert

    NACH7 (CHRNA7 Human)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1alpha

    Available Since

    May 5, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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