168,321 results

1. pSG5-EGFP

   Plasmid

   #49246

   Purpose

   encodes enhanced green fluorescent protein (EGFP)

   Depositor

   Rebecca Riggins

   Insert

   EGFP
   

   Expression

   Mammalian

   Promoter

   SV40

   Available Since

   Nov. 25, 2013

   Availability

   Academic Institutions and Nonprofits only

2. TRE-mClover3-TDP-43ΔNLS

   Plasmid

   #214917

   Purpose

   inducible expression of TDP-43 harboring mutant NLS and N-terminal mClover3. Expression yields cytosolic TDP-43 that forms peinuclear puncta

   Depositor

   Ophir Shalem

   Article

   Sweeney et al PLoS Genet. 2024 Feb 5;20(2):e1011138. d

   Insert

   TDP-43ΔNLS (TARDBP Human)
   

   Use

   Lentiviral

   Tags

   mClover3

   Mutation

   mutant NLS GCAGCAGCAATGGATGAGACAGATGCTTCATCAGCAGT…

   Available Since

   March 11, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. Toronto KnockOut version 3 library (TKOv3)

   Pooled Library

   #90294

   Purpose

   Optimized library offering improved accuracy, efficiency, and scalability for CRISPR screens compared to TKOv1

   Depositor

   Jason Moffat

   Article

   Hart et al G3 (Bethesda). 2017 Aug 7;7(8):2719-2727

   Available Since

   May 5, 2017

   Availability

   Academic Institutions and Nonprofits only

4. pFastBac HT JS-Rab10wt

   Plasmid

   #135557

   Purpose

   Express mouse Rab10wt in Sf9 cells, the resulted plasmid encodes an N-terminally His6-tagged Munc18c protein with a tobacco etch virus (TEV) cleavage site between the His6 tag and Rab10wt.

   Depositor

   Jingshi Shen

   Article

   Gulbranson et al Proc Natl Acad Sci U S A. 2017 Sep 26;11

   Insert

   Rab10 (Rab10 Mouse)
   

   Tags

   6xHis tag, TEV site

   Expression

   Insect

   Promoter

   polyhedrin promoter

   Available Since

   Feb. 20, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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5. pIBA2-Int-SpyTag

   Plasmid

   #198038

   Purpose

   Expression plasmid for producing SpyTagged stalled secretion variant of intimin

   Depositor

   Jack Leo

   Article

   Duodu et al Methods Mol Biol. 2024;2778:53-63. doi:

   Insert

   eaeA
   

   Tags

   SpyTag

   Expression

   Bacterial

   Mutation

   lacks native signal peptide (amino acids 1-39)

   Promoter

   tet

   Available Since

   March 18, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. pCFD4d-U6-1:white1-U6-3:white1

   Plasmid

   #84005

   Purpose

   pCFD4d expresses white sgRNA-1 from a U6:3 promoter and the same white sgRNA-1 from a U6:1 promoter

   Depositor

   Phillip Zamore

   Article

   Ge et al G3 (Bethesda). 2016 Aug 26. pii: g3.116.

   Inserts

   white sgRNA-1
   white sgRNA-1
   

   Use

   CRISPR

   Promoter

   U6:1, U6:3, and dU6-1; dU6-3

   Available Since

   Jan. 9, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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7. SCP1-CMVenh-luc

   Plasmid

   #22216

   Depositor

   James Kadonaga

   Article

   Juven-Gershon et al Nat Methods. 2006 Nov . 3(11):917-22.

   Insert

   SCP1 (super core promoter 1)-CMVenh luciferase
   

   Use

   Luciferase

   Expression

   Mammalian

   Available Since

   April 30, 2010

   Availability

   Academic Institutions and Nonprofits only

   

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8. pYZ183

   Plasmid

   #98425

   Purpose

   Pombe Expression Vector - nmt41 promoter with empty BsaI-pad - no marker

   Depositor

   Jef Boeke

   Article

   Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   Feb. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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9. pYZ184

   Plasmid

   #98426

   Purpose

   Pombe Expression Vector - nmt81 promoter with empty BsaI-pad - no marker

   Depositor

   Jef Boeke

   Article

   Zhao et al G3 (Bethesda). 2018 Jan 10. pii: g3.117.

   Type

   Empty backbone

   Expression

   Yeast

   Available Since

   Feb. 5, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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10. TFORF3549

    Plasmid

    #145025

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens. This is the control vector for the collection.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    GFP
    

    Use

    Lentiviral

    Expression

    Mammalian

    Promoter

    EF1a

    Available Since

    Jan. 5, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pOPINE-3C-mCherry

    Plasmid

    #214060

    Purpose

    Expression of fluorescent fusion proteins

    Depositor

    Ray Owens

    Insert

    mCherry
    

    Tags

    Hexahistidine

    Expression

    Bacterial, Insect, and Mamm…

    Available Since

    Feb. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCI-syn-fRCaMP2

    Plasmid

    #125246

    Purpose

    Expresses red fluorescent calcium sensor in hippocampal slices

    Depositor

    Katalin Torok

    Article

    Kerruth et al J Biol Chem. 2019 Mar 15;294(11):3934-39

    Insert

    fRCaMP2
    

    Expression

    Mammalian

    Mutation

    D396A

    Promoter

    synapsin

    Available Since

    Nov. 25, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. pD434-SR mouse Alpha-synuclein

    Plasmid

    #89073

    Purpose

    bacterial expression of mouse Alpha-synuclein

    Depositor

    Michael J Fox Foundation MJFF

    Insert

    Alpha-synuclein (Snca Mouse)
    

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 17, 2017

    Availability

    Industry, Academic Institutions, and Nonprofits

    

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14. pVITRO-HPV8 L1L2

    Plasmid

    #52598

    Purpose

    Expresses HPV8 L1 and L2 in mammalian cells

    Depositor

    Richard Roden

    Article

    Kwak et al PLoS One. 2014 May 9;9(5):e97232. doi: 1

    Inserts

    HPV8 L1
    HPV8 L2
    

    Expression

    Mammalian

    Available Since

    May 20, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCAG-EGFP-C-IFT74

    Plasmid

    #218730

    Purpose

    Expresses N-terminally EGFP-tagged IFT74 in mammalian cells

    Depositor

    Kazuhisa Nakayama

    Article

    Nishijima et al Mol Biol Cell. 2017 Jun 15;28(12):1652-1

    Insert

    IFT74 (IFT74 Human)
    

    Tags

    EGFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    May 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCDNA3.1 (-) + codon optimized Brn3a

    Plasmid

    #86829

    Purpose

    mammalian expression of the transcription factor Brn3a

    Depositor

    Carl Novina

    Article

    Schmidt et al Cell Rep. 2016 May 31;15(9):2025-37. doi

    Insert

    Brn3a (POU4F1 Human)
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 2, 2017

    Availability

    Academic Institutions and Nonprofits only

    

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17. pGEX-4T1 mouse PARG (439-959)

    Plasmid

    #132615

    Purpose

    expresses mouse PARG(439-959) in E. coli.

    Depositor

    Wenqing Xu

    Article

    Wang et al PLoS One. 2014 Jan 21;9(1):e86010. doi:

    Insert

    PARG (439-959)
    

    Expression

    Bacterial

    Available Since

    Oct. 17, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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18. pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA (AAV8)

    Viral Prep

    #20297-AAV8

    Purpose

    Ready-to-use AAV8 particles produced from pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA (#20297). In addition to the viral particles, you will also receive purified pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA plasmid DNA. Humanized channelrhodopsin H134R mutant fused to mCherry, driven by the EF1a promoter. Cre-dependent. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Karl Deisseroth

    Promoter

    EF1a

    Tags

    mCherry (Cre-dependent)

    Available Since

    Dec. 18, 2017

    Availability

    Academic Institutions and Nonprofits only

19. pCDNA5/FRT-Lyn11-mEGFP-cpHalo∆-(GGS)9-FKBP-P2A-Hpep3-(GGS)3-FRB-mScarlett

    Plasmid

    #205703

    Purpose

    CMV driven co-expression of split HaloTag FKBP- and FRB-fusions (Lyn11-EGFP-FKBP-cpHalo∆ and Hpep1-FRB-mScarlett) in mammalian cell lines

    Depositor

    Kai Johnsson

    Article

    Huppertz et al Science. 2024 Feb 23;383(6685):890-897.

    Insert

    Lyn11-EGFP-FKBP-cpHalo∆-P2A-Hpep1-FRB-mScarlett
    

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Feb. 23, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. HC87 pEYFPN1 anti-Homer1 nanobody

    Plasmid

    #135223

    Purpose

    Mammalian expression of anti-Homer1 (Mouse) recombinant llama nanobody for use as an intrabody

    Depositor

    James Trimmer

    Article

    Dong et al Elife. 2019 Sep 30;8. pii: 48750. doi: 1

    Insert

    anti-Homer1 (Mouse) recombinant llama nanobody HC87 (Homer1 L. glama (llama))
    

    Tags

    6xHis-HA-EYFP

    Expression

    Mammalian

    Available Since

    Dec. 18, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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