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A Neuron-Optimized CRISPR/dCas9 Activation System for Robust and Specific Gene Regulation.
Savell KE, Bach SV, Zipperly ME, Revanna JS, Goska NA, Tuscher JJ, Duke CG, Sultan FA, Burke JN, Williams D, Ianov L, Day JJ
eNeuro. 2019 Mar 7;6(1). pii: eN-MNT-0495-18. doi: 10.1523/ENEURO.0495-18.2019. eCollection 2019 Jan-Feb.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
114195lenti EF1a-FLAG-dCas9-VPRLentivirus compatible dCas9-VPR construct driven by the EF1a promoter
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114196lenti SYN-FLAG-dCas9-VPRLentivirus compatible dCas9-VPR construct driven by the human SYN promoter
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114197lenti CAG-FLAG-dCas9-VPRLentivirus compatible dCas9-VPR construct driven by the CAG promoter
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114199lenti U6-sgRNA/EF1a-mCherryLentivirus compatible SpCas9/dCas9 sgRNA scaffold driven by the U6 promoter and mCherry driven by the EF1a promoter
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Antibodies from Article