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(Plasmid #51628)


Item Catalog # Description Quantity Price (USD)
Plasmid 51628 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone size w/o insert (bp) 6300
  • Modifications to backbone
    replace amp to kan
  • Vector type
    Bacterial Expression ; Red recombinase and I-SceI endonuclease

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin, 25 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Growth instructions
    Culture the plate in 30 C or 28 C for 24h or up to 48h to see if any colonies appear. LB +1% glucose plate, or SOC plate improve growth.
  • Copy number
    Low Copy


  • Gene/Insert name
  • Alt name
    mitochondrial intron of Saccharomyces cerevisiae
  • Species
    S. cerevisiae (budding yeast)
  • Insert Size (bp)
  • Entrez Gene
    SCEI (a.k.a. Q0160)
  • Promoter trc

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site NcoI (not destroyed)
  • 3′ cloning site NcoI (not destroyed)
  • 5′ sequencing primer none
  • (Common Sequencing Primers)

Resource Information

  • Supplemental Documents
  • Addgene Notes
  • A portion of this plasmid was derived from a plasmid made by
    pUC19RP12 Nucl. Acids Res. (1999) 27 (22): 4409-4415. György Pósfai, Institute of Biochemistry, Biological Research Center, H-6701 Szeged, Hungary
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pREDTKI was a gift from Sheng Yang (Addgene plasmid # 51628 ; ; RRID:Addgene_51628)
  • For your References section:

    High-Efficiency Scarless Genetic Modification in Escherichia coli by Using Lambda Red Recombination and I-SceI Cleavage. Yang J, Sun B, Huang H, Jiang Y, Diao L, Chen B, Xu C, Wang X, Liu J, Jiang W, Yang S. Appl Environ Microbiol. 2014 Jul 1;80(13):3826-3834. Epub 2014 Apr 18. 10.1128/AEM.00313-14 PubMed 24747889