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(Plasmid #51655)


Item Catalog # Description Quantity Price (USD)
Plasmid 51655 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.


  • Vector backbone
    pMD18-T Simple
  • Backbone manufacturer
  • Backbone size w/o insert (bp) 2600
  • Vector type
    genetic modification

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy


  • Gene/Insert name
    apramycin antibiotic resistance gene
  • Insert Size (bp)
  • Promoter unknown

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    pIJ773 (Gust B, Kieser T, Chater KF: PCR targeting system in Streptomyces coelicolor A3(2). John Innes Centre 2002. )
  • Articles Citing this Plasmid

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pMDIAI was a gift from Sheng Yang (Addgene plasmid # 51655 ; ; RRID:Addgene_51655)
  • For your References section:

    High-Efficiency Scarless Genetic Modification in Escherichia coli by Using Lambda Red Recombination and I-SceI Cleavage. Yang J, Sun B, Huang H, Jiang Y, Diao L, Chen B, Xu C, Wang X, Liu J, Jiang W, Yang S. Appl Environ Microbiol. 2014 Jul 1;80(13):3826-3834. Epub 2014 Apr 18. 10.1128/AEM.00313-14 PubMed 24747889