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pcDNA3.3-Nm-dCas9-p300 Core
(Plasmid #61365)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 61365 Plasmid sent as bacteria in agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pcDNA3.3
  • Backbone manufacturer
    Invitrogen; Life Technologies
  • Total vector size (bp) 10579
  • Vector type
    Mammalian Expression, CRISPR, Synthetic Biology
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    Nm-dCas9-p300 Core
  • Species
    H. sapiens (human), Synthetic; Neisseria meningiditis
  • Insert Size (bp)
    5175
  • Mutation
    D16A, D587A, H588A, N611A in Nm Cas9
  • GenBank ID
    WP_002235162.1 NP_001420.2
  • Entrez Gene
    EP300 (a.k.a. KAT3B, RSTS2, p300)
  • Promoter CMV
  • Tags / Fusion Proteins
    • NLS (C terminal on insert)
    • HA tag (C terminal on insert)

Cloning Information

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pcDNA3.3-Nm-dCas9-p300 Core was a gift from Charles Gersbach (Addgene plasmid # 61365)
  • For your References section:

    Epigenome editing by a CRISPR-Cas9-based acetyltransferase activates genes from promoters and enhancers. Hilton IB, D'Ippolito AM, Vockley CM, Thakore PI, Crawford GE, Reddy TE, Gersbach CA. Nat Biotechnol. 2015 May;33(5):510-7. doi: 10.1038/nbt.3199. Epub 2015 Apr 6. 10.1038/nbt.3199 PubMed 25849900