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179,919 results

Showing: 4981 - 5000 of 179919 results

  15. pMRX-IB-pSu9-HaloTag7-mGFP

    Plasmid
    #184905
    Purpose
    Stable expression of pSu9-HaloTag7-mGFP in mammalian cells to measure mitophagy activity
    Depositor
    Noboru Mizushima
    Publication
    Yim et al Elife. 2022 Aug 8;11. pii: 78923. doi: 1
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Presequence of Fo-ATPase subunit 9
    Tags
    HaloTag7 and monomeric EGFP
    Expression
    Mammalian
    Available since
    June 14, 2022
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    6 Articles
    184905-plasmid-map-sequence-id-364106

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  16. Luc-PS9

    Plasmid
    #177942
    Purpose
    Transient mammalian expression of Luc2 (firefly luciferase) along with SARS-CoV-2 sequence 20080-21171 (PS9) within the 3'UTR. Resulting transcript is packaged into SARS-CoV-2 virus-like particles.
    Depositor
    Jennifer Doudna
    Publication
    Syed et al Science. 2021 Nov 4:eabl6184. doi: 10.11
    Has service
    50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNA
    Insert
    Luc2 (ORF1ab )
    Expression
    Mammalian
    Available since
    Nov. 17, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    177942-plasmid-map-sequence-id-348206

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  18. pET-pAG-Hia5

    Plasmid
    #174373
    Purpose
    Plasmid for IPTG-inducible expression and purification of protein A/G-Hia5-6xHis (pAG-Hia5) for antibody directed DNA (adenine) methylation (as in DiMeLo-seq)
    Depositor
    Aaron Straight
    Publication
    Altemose et al Nat Methods. 2022 Jun;19(6):711-723. doi
    Insert
    protein AG - hia5
    Tags
    6xHis and Protein A/G
    Expression
    Bacterial
    Available since
    Sept. 6, 2021
    Availability
    Academic Institutions and Nonprofits only
    174373-plasmid-map-sequence-id-341861

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  19. pLenti-Tet-coBxb1-2A-BFP_IRES-iCasp9-2A-Blast_rtTA3

    Plasmid
    #171588
    Purpose
    Lentivector encoding a DNA recombinase landing pad with Bxb1 integrase and iCasp9 negative selection behind a Tet-inducible promoter
    Depositor
    Kenneth Matreyek
    Publication
    Shukla et al PLoS Pathog. 2021 Jul 16;17(7):e1009715.
    Inserts
    Bxb1 integrase
    iCasp9
    BFP
    rtTA3G
    Use
    Lentiviral and Synthetic Biology
    Expression
    Mammalian
    Available since
    July 21, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    5 Articles
    171588-plasmid-map-sequence-id-335078

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  20. pET-PE2-His (IK1822)

    Plasmid
    #170103
    Purpose
    T7 promoter bacterial expression plasmid for PE2 (nSpCas9(H840A)-MMLV-RT) with C-terminal 6xHis-tag and bipartite NLS
    Depositor
    Keith Joung
    Publication
    Petri et al Nat Biotechnol. 2021 Apr 29. pii: 10.103
    Insert
    bpNLS-nSpCas9(H840A)-MMLVRT-bpNLS-6xHis
    Use
    CRISPR
    Tags
    6xHis tag
    Expression
    Bacterial
    Mutation
    Bacteria codon-optimized nSpCas9 (H840A) & M-…
    Promoter
    T7
    Available since
    May 13, 2021
    Availability
    Academic Institutions and Nonprofits only
    Cited by
    3 Articles
    170103-plasmid-map-sequence-id-332603

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Showing: 4981 - 5000 of 179919 results