161,424 results

1. pET51b-His-TEV-HaloTag7

   Plasmid

   #167266

   Purpose

   Overexpression of HaloTag7 in E. coli

   Depositor

   Kai Johnsson

   Article

   Wilhelm et al Biochemistry. 2021 Aug 24;60(33):2560-25

   Insert

   HaloTag7
   

   Use

   Tags

   His x10

   Expression

   Bacterial

   Mutation

   Promoter

   T7

   Available since

   April 14, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. mouse b3 full length

   Plasmid

   #217814

   Purpose

   Full length mouse integrin b3 expression

   Depositor

   Timothy Springer

   Article

   Yuxin et al bioRxiv 2024.01.26.577394

   Insert

   ITGB3 (Itgb3 Mouse)
   

   Use

   Tags

   P2A-mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   May 15, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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3. LSL-Cas9-Rosa26TV

   Plasmid

   #61408

   Purpose

   Cre-dependent Cas9 expression plasmid and targeting vector for the mouse Rosa26 locus. Cas9 expression is Cre-dependent and the targeting vector has positive (Neo) and negative (DTA) selection.

   Depositor

   Feng Zhang

   Article

   Platt et al Cell. 2014 Sep 24. pii: S0092-8674(14)01

   Inserts

   Cas9
   EGFP
   

   Use

   CRISPR, Cre/Lox, and Mouse Targeting

   Tags

   3xFLAG and P2A

   Expression

   Mammalian

   Mutation

   human codon optimized

   Promoter

   CAG

   Available since

   Jan. 15, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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4. pLVX-RT004-LaG30VB

   Plasmid

   #163384

   Purpose

   2nd generation lentiviral vector to express anti-GFP(LaG30) module in the G-baToN system

   Depositor

   Monte Winslow

   Article

   Tang et al Elife. 2020 Oct 7;9. pii: 61080. doi: 10

   Insert

   LaG30-VEEGFRtm-BFP (LaG30VB)
   

   Use

   Lentiviral

   Tags

   BFP

   Expression

   Bacterial and Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Jan. 11, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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5. pLenti-CMV-Puro-AAVR-FLAG

   Plasmid

   #166716

   Purpose

   Vector expressing AAVR (KIAA0319L) with a C-terminal Flag tag

   Depositor

   Jan Carette

   Article

   Pillay et al Nature. 2016 Feb 4;530(7588):108-12. doi

   Insert

   AAVR-FLAG (KIAA0319L Human)
   

   Use

   Lentiviral

   Tags

   FLAG

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   June 15, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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6. CAG-BFP-sesRNA-tTA2-WPRE-pA

   Plasmid

   #192070

   Purpose

   Expression of BFP, sesRNA in mammalian cells, with tTA2 as efRNA.

   Depositor

   Josh Huang

   Article

   Qian et al Nature. 2022 Oct;610(7933):713-721. doi:

   Insert

   sesRNA for tdTom
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   CAG

   Available since

   Feb. 2, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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7. TFORF0500

   Plasmid

   #143709

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   PPARGC1A (PPARGC1A Human)
   

   Use

   Lentiviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   EF1a

   Available since

   Sept. 28, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pBabe puro IRES-EGFP

   Plasmid

   #14430

   Depositor

   L. Miguel Martins

   Has Service

   Cloning Grade DNA

   Insert

   IRES-EGFP
   

   Use

   Retroviral

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2007

   Availability

   Academic Institutions and Nonprofits only

   

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9. pPB-cT3G-cERP2-RUNX2

   Plasmid

   #192926

   Purpose

   Barcoded piggybac transposon vector with Dox-inducible expression of RUNX2

   Depositor

   George Church

   Article

   Pierson Smela et al Elife. 2023 Feb 21;12:e83291. doi: 10.75

   Insert

   RUNX2 (RUNX2 Human)
   

   Use

   Tags

   HA

   Expression

   Mammalian

   Mutation

   Promoter

   TET3G-miniCMV

   Available since

   Feb. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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10. pVITRO1-Trastuzumab-IgG1/κ

    Plasmid

    #61883

    Purpose

    Expresses HER2/neu receptor specific humanized IgG1/κ antibody isotype (Trastuzumab-IgG1/κ)

    Depositor

    Andrew Beavil

    Article

    Dodev et al Sci Rep. 2014 Jul 30;4:5885. doi: 10.103

    Inserts

    HER2/neu receptor specific humanized Gamma 1 heavy chain expression cassette
    HER2/neu receptor specific humanized kappa light chain expression cassette
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    mEF1 Prom and rEF1 Prom

    Available since

    March 17, 2015

    Availability

    Academic Institutions and Nonprofits only

    

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11. pcDNA3.1-N-Myc_C223S USP7

    Plasmid

    #131243

    Purpose

    Mammalian expression of catalytically inactive Myc-tagged USP7

    Depositor

    Roger Woodgate

    Article

    Ashton et al J Mol Biol. 2021 Jan 22;433(2):166733. d

    Insert

    Ubiquitin carboxyl-terminal hydrolase 7 (USP7 Human)
    

    Use

    Tags

    Myc

    Expression

    Mammalian

    Mutation

    Contains a point mutation that converts USP7 cyst…

    Promoter

    Available since

    Dec. 17, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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12. TFORF2904

    Plasmid

    #143388

    Purpose

    Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

    Depositor

    Feng Zhang

    Article

    Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

    Insert

    DACH1 (DACH1 Human)
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    EF1a

    Available since

    July 11, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. Mouse metabolism knockout library

    Pooled Library

    #160129

    Purpose

    CRISPR library targets mouse metabolic genes

    Depositor

    Kivanc Birsoy

    Article

    Zhu et al Cell Metab. 2020 Oct 28. pii: S1550-4131

    Expression

    Mammalian

    Species

    Mus musculus

    Use

    CRISPR and Lentiviral

    Available since

    Nov. 10, 2020

    Availability

    Academic Institutions and Nonprofits only

14. pCMV-15F11-HA-mEGFP

    Plasmid

    #129590

    Purpose

    The encoded protein is the anti-HA scFv (anti-HA frankenbody) fused with the mEGFP. It can be used to track mature and nascent HA tagged proteins in living organism.

    Depositor

    Tim Stasevich

    Article

    Zhao et al Nat Commun. 2019 Jul 3;10(1):2947. doi:

    Insert

    Anti-HA frankenbody-mEGFP (15F11-HA scFv-mEGFP)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Aug. 15, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pCMV-dCas13-inactive M3M14nes

    Plasmid

    #157855

    Purpose

    Targeted m6A RNA methylation in mammalian cells, methyltransferase-inactive mutant

    Depositor

    David Liu

    Article

    Wilson et al Nat Biotechnol. 2020 Jun 29. pii: 10.103

    Insert

    dCas13-inactive M3M14nes (METTL3, METTL14 Cas13b is from Prevotella sp. P5-125; METTL3 and METTL14 are from H. sapiens)
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    PspCas13b Δ984-1090 H133A; METTL3 D395A

    Promoter

    Available since

    Aug. 4, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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16. Mouse GeCKO Lentiviral sgRNA Library (LentiGuide-Puro)

    Pooled Library

    #1000000053

    Purpose

    CRISPR gRNA knockout pooled library for targeting the mouse genome

    Depositor

    Feng Zhang

    Available since

    July 23, 2014

    Availability

    Academic Institutions and Nonprofits only

17. pGL2-NOS2Promoter-Luciferase

    Plasmid

    #19296

    Depositor

    Charles Lowenstein

    Article

    Lowenstein et al Proc Natl Acad Sci U S A. 1993 Oct 15. 9

    Insert

    NOS2 Promoter (Nos2 Mouse)
    

    Use

    Luciferase

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 12, 2008

    Availability

    Academic Institutions and Nonprofits only

    

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18. H2A-mScarletI-P2A-Akt-KTR-mTurquoise2-P2A-ERK-KTR-mNeonGreen

    Plasmid

    #129631

    Purpose

    Visualization of Akt and ERK activity

    Depositor

    Dorus Gadella

    Article

    Chavez-Abiega et al J Cell Sci. 2022 Mar 15;135(6):jcs259685

    Insert

    H2A-mScarletI-P2A-Akt-KTR-mTurquoise2-P2A-ERK-KTR-mNeonGreen
    

    Use

    Tags

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    July 28, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. pLVX-opto-mCasp1

    Plasmid

    #208781

    Purpose

    Inducibly expresses mouse caspase1 fused to light activatable Cry2

    Depositor

    Petr Broz

    Article

    Shkarina et al J Cell Biol. 2022 Jun 6;221(6). pii: 213

    Insert

    mCherry mCaspase1 Cry2 (Casp1 Mouse)
    

    Use

    Lentiviral

    Tags

    mCherry, Cry2

    Expression

    Mammalian

    Mutation

    Promoter

    Available since

    Nov. 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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20. Mucolipin1-pEGFP C3

    Plasmid

    #62960

    Purpose

    full length human Mucolipin-1 cloned into pEGFP C3

    Depositor

    Paul Luzio

    Article

    Pryor et al Traffic. 2006 Oct;7(10):1388-98.

    Insert

    Mucolipin-1 (MCOLN1 Human)
    

    Use

    Tags

    EGFP

    Expression

    Mammalian

    Mutation

    Mucolipin-1 has 1 silent mutation compared to the…

    Promoter

    CMV

    Available since

    May 28, 2015

    Availability

    Academic Institutions and Nonprofits only