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pEN435 - pCAGGS-TagBFP-hGeminin-2A-mCherry-hCdt1-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt Tigre targeting
(Plasmid #92139)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 92139 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pEN395 (Addgene #92141)
  • Vector type
    Mammalian Expression, Mouse Targeting
  • Selectable markers
    Puromycin

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    chromatin licensing and DNA replication factor 1
  • Species
    H. sapiens (human)
  • Entrez Gene
    GMNN (a.k.a. Gem, MGORS6)
  • Entrez Gene
    CDT1 (a.k.a. DUP, RIS2)

Resource Information

Depositor Comments

use with pX330-EN1201 (Cas9 + sgRNA against mouse TIGRE acceptor locus)

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pEN435 - pCAGGS-TagBFP-hGeminin-2A-mCherry-hCdt1-rbgpA-Frt-PGK-EM7-PuroR-bpA-Frt Tigre targeting was a gift from Benoit Bruneau (Addgene plasmid # 92139 ; http://n2t.net/addgene:92139 ; RRID:Addgene_92139)
  • For your References section:

    Targeted Degradation of CTCF Decouples Local Insulation of Chromosome Domains from Genomic Compartmentalization. Nora EP, Goloborodko A, Valton AL, Gibcus JH, Uebersohn A, Abdennur N, Dekker J, Mirny LA, Bruneau BG. Cell. 2017 May 18;169(5):930-944.e22. doi: 10.1016/j.cell.2017.05.004. 10.1016/j.cell.2017.05.004 PubMed 28525758