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PurposeSMX_DEST vector with 3 cassettes
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Depositing Lab
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | ||
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Plasmid | 62451 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $75 |
This material is available to academics and nonprofits only.
Backbone
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Vector backboneSMX_DEST
- Backbone size w/o insert (bp) 1950
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Vector typeSynthetic Biology
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin
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Growth Temperature37°C
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Growth Strain(s)Mach1
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Copy numberHigh Copy
Gene/Insert 1
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Gene/Insert nameminPGK::Citrine-Geminin(1-110)-bGHpA (PGKEnhancer)x5
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SpeciesSynthetic
Cloning Information for Gene/Insert 1
- Cloning method Unknown
- 5′ sequencing primer TTACCGCCTTTGAGTGAG (Common Sequencing Primers)
Gene/Insert 2
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Gene/Insert nameminPGK::Cherry-hCdt1(30-120)-bGHpA
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SpeciesSynthetic
Gene/Insert 3
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Gene/Insert namePGK::HygroR-bGpA
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SpeciesSynthetic
Resource Information
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Supplemental Documents
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Terms and Licenses
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Industry Terms
- Not Available to Industry
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Articles Citing this Plasmid
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
ES-FUCCI was a gift from Pierre Neveu (Addgene plasmid # 62451 ; http://n2t.net/addgene:62451 ; RRID:Addgene_62451) -
For your References section:
MXS-Chaining: A Highly Efficient Cloning Platform for Imaging and Flow Cytometry Approaches in Mammalian Systems. Sladitschek HL, Neveu PA. PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015. PONE-D-15-01385 [pii] PubMed 25909630