ES-FUCCI
(Plasmid #62451)

• Purpose: SMX_DEST vector with 3 cassettes
• Depositing Lab: Pierre Neveu
• Publication: Sladitschek et al PLoS One. 2015 Apr 24;10(4):e0124958. doi: 10.1371/journal.pone.0124958. eCollection 2015.

Backbone

• Vector backbone: SMX_DEST
  (Search Vector Database)
• Backbone size w/o insert (bp): 1950
• Vector type: Synthetic Biology

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Mach1
• Copy number: High Copy

Gene/Insert 1

• Gene/Insert name: minPGK::Citrine-Geminin(1-110)-bGHpA (PGKEnhancer)x5
• Species: Synthetic

Cloning Information for Gene/Insert 1

• Cloning method: Unknown
• 5′ sequencing primer: TTACCGCCTTTGAGTGAG

Gene/Insert 2

• Gene/Insert name: minPGK::Cherry-hCdt1(30-120)-bGHpA
• Species: Synthetic

Gene/Insert 3

• Gene/Insert name: PGK::HygroR-bGpA
• Species: Synthetic

Resource Information

• Supplemental Documents:
  • Annotated Genbank file
• Articles Citing this Plasmid:
  • 11 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
  • Ancillary Agreement for Plasmids Containing FP Materials
  • Takara Bio Limited Use Label License (formerly Clontech)
• Industry Terms:
  • Not Available to Industry