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Programmed DNA destruction by miniature CRISPR-Cas14 enzymes.
Harrington LB, Burstein D, Chen JS, Paez-Espino D, Ma E, Witte IP, Cofsky JC, Kyrpides NC, Banfield JF, Doudna JA
Science. 2018 Oct 18. pii: science.aav4294. doi: 10.1126/science.aav4294. PubMed Article

Plasmids from Article

ID Plasmid Purpose  
112500pLBH531_MBP-Cas14a1 expressionExpresses MBP fused to Cas14a1
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112501pLBH545_Tet-Cas14a1_LocusExpresses Cas14a1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter
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112502pLBH559_Tet-HisCas14a1LocusExpresses 10xHis-Cas14a1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter
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112503pLBH546_Tet-Cas14b1LocusExpresses Cas14b1 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter
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112504pLBH547_Tet-Cas14b2LocusExpresses Cas14b2 locus (including crRNA and tracrRNA) under control of a tetracycline inducible promoter
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112505pLBH532_MBP-Cas14b1 expressionExpresses 10xHis-MBP fused to Cas14b1 under t7 promoter
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112506pLBH533_MBP-Cas14b2 expressionExpresses 10xHis-MBP fused to Cas14b2 under t7 promoter
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