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mCh-Climp63
(Plasmid #136293)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 136293 Standard format: Plasmid sent in bacteria as agar stab 1 $75

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pmCherry-N1
  • Backbone manufacturer
    Clontech
  • Backbone size w/o insert (bp) 4722
  • Vector type
    Mammalian Expression
  • Selectable markers
    Neomycin (select with G418)

Growth in Bacteria

  • Bacterial Resistance(s)
    Kanamycin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    Cytoskeletal-linking protein 63
  • Alt name
    CLIMP-63
  • Alt name
    CKAP4
  • Species
    M. musculus (mouse)
  • Insert Size (bp)
    1725
  • GenBank ID
    NM_175451.1
  • Entrez Gene
    Ckap4 (a.k.a. 5630400A09Rik, CLIMP-63, P63)
  • Promoter CMV
  • Tag / Fusion Protein
    • mCherry (N terminal on backbone)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site XhoI (not destroyed)
  • 3′ cloning site EcoRI (not destroyed)
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer SV40pA-R
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    mCh-Climp63 was a gift from Gia Voeltz (Addgene plasmid # 136293 ; http://n2t.net/addgene:136293 ; RRID:Addgene_136293)
  • For your References section:

    The reticulon and DP1/Yop1p proteins form immobile oligomers in the tubular endoplasmic reticulum. Shibata Y, Voss C, Rist JM, Hu J, Rapoport TA, Prinz WA, Voeltz GK. J Biol Chem. 2008 Jul 4. 283(27):18892-904. 10.1074/jbc.M800986200 PubMed 18442980