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Precise and efficient genome editing in Klebsiella pneumoniae using CRISPR-Cas9 and CRISPR-assisted cytidine deaminase.
Wang Y, Wang S, Chen W, Song L, Zhang Y, Shen Z, Yu F, Li M, Ji Q
Appl Environ Microbiol. 2018 Sep 14. pii: AEM.01834-18. doi: 10.1128/AEM.01834-18.
PubMed Article

Plasmids from Article

ID Plasmid Purpose  
117231pCasKP-aprBacterial expression of Cas9 nuclease and lambda-Red system in Klebsiella Pneumoniae
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117232pCasKP-hphBacterial expression of Cas9 nuclease and lambda-Red system in Klebsiella Pneumoniae
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117233pSGKP-kmA sgRNA expression plasmid for genome editing in Klebsiella Pneumoniae
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117234pSGKP-speA sgRNA expression plasmid for genome editing in Klebsiella Pneumoniae
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117235pBECKP-kmA cytidine deaminase-mediated base-editing plasmid in Klebsiella Pneumoniae
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117236pBECKP-spencas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase
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