Precise and efficient genome editing in Klebsiella pneumoniae using CRISPR-Cas9 and CRISPR-assisted cytidine deaminase.
Wang Y, Wang S, Chen W, Song L, Zhang Y, Shen Z, Yu F, Li M, Ji Q
Appl Environ Microbiol. 2018 Sep 14. pii: AEM.01834-18. doi: 10.1128/AEM.01834-18. PubMed Article
Wang Y, Wang S, Chen W, Song L, Zhang Y, Shen Z, Yu F, Li M, Ji Q
Appl Environ Microbiol. 2018 Sep 14. pii: AEM.01834-18. doi: 10.1128/AEM.01834-18. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 117231 | pCasKP-apr | Bacterial expression of Cas9 nuclease and lambda-Red system in Klebsiella Pneumoniae |
| 117232 | pCasKP-hph | Bacterial expression of Cas9 nuclease and lambda-Red system in Klebsiella Pneumoniae |
| 117233 | pSGKP-km | A sgRNA expression plasmid for genome editing in Klebsiella Pneumoniae |
| 117234 | pSGKP-spe | A sgRNA expression plasmid for genome editing in Klebsiella Pneumoniae |
| 117235 | pBECKP-km | A cytidine deaminase-mediated base-editing plasmid in Klebsiella Pneumoniae |
| 117236 | pBECKP-spe | ncas9-BEC, fusion protein of rAPOBEC1 and spCas9 nickase |