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pSmartBAC-S
(Plasmid #107268)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 107268 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pSmartBAC (EU101022.1)
  • Backbone manufacturer
    Lucigen Corporation
  • Backbone size (bp) 7648
  • Modifications to backbone
    We first restriction digested pSmartBAC (EU101022.1) with ApaI, end-repaired the DNA to make it blunt and dephosphorylated the digested pSmartBAC vector ends. A synthesized and blunt-ended gene cassette that contained the ɸC31 integrase (int), attachment site (attP) and aac3(IV) that encodes for apramycin-resistance (4,793bp) was ligated to the linearized pSmartBAC-S and apramycin-resistant transformants in E. coli 10G were selected.
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Chloramphenicol, 25 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Growth instructions
    Arabinose induction of copy number
  • Copy number
    Low Copy

Cloning Information

Resource Information

  • Supplemental Documents
  • A portion of this plasmid was derived from a plasmid made by
    The gene cassette was synthesized by Dr. ChengCang Wu, formerly of Lucigen. More information if needed could be obtained directly from him at [email protected]

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Also carries apramycin resistance cassette. Selection for chloramphenicol and/or apramycin resistance will maintain the plasmid.

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pSmartBAC-S was a gift from Ron Godiska & Mark Liles (Addgene plasmid # 107268 ; http://n2t.net/addgene:107268 ; RRID:Addgene_107268)