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Purpose(Empty Backbone) Broad host range bacterial artificial chromosome vector
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Depositing Labs
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Sequence Information
Ordering
Item | Catalog # | Description | Quantity | Price (USD) | |
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Plasmid | 107268 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepSmartBAC (EU101022.1)
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Backbone manufacturerLucigen Corporation
- Backbone size (bp) 7648
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Modifications to backboneWe first restriction digested pSmartBAC (EU101022.1) with ApaI, end-repaired the DNA to make it blunt and dephosphorylated the digested pSmartBAC vector ends. A synthesized and blunt-ended gene cassette that contained the ɸC31 integrase (int), attachment site (attP) and aac3(IV) that encodes for apramycin-resistance (4,793bp) was ligated to the linearized pSmartBAC-S and apramycin-resistant transformants in E. coli 10G were selected.
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Vector typeBacterial Expression
Growth in Bacteria
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Bacterial Resistance(s)Chloramphenicol, 25 μg/mL
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Growth Temperature37°C
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Growth Strain(s)DH5alpha
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Growth instructionsArabinose induction of copy number
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Copy numberLow Copy
Cloning Information
- Cloning method Restriction Enzyme
- 5′ sequencing primer M13F
- 3′ sequencing primer M13R (Common Sequencing Primers)
Resource Information
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Supplemental Documents
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A portion of this plasmid was derived from a plasmid made byThe gene cassette was synthesized by Dr. ChengCang Wu, formerly of Lucigen. More information if needed could be obtained directly from him at [email protected]
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
Also carries apramycin resistance cassette. Selection for chloramphenicol and/or apramycin resistance will maintain the plasmid.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pSmartBAC-S was a gift from Ron Godiska & Mark Liles (Addgene plasmid # 107268 ; http://n2t.net/addgene:107268 ; RRID:Addgene_107268)