Spy&Go purification of SpyTag-proteins using pseudo-SpyCatcher to access an oligomerization toolbox.
Khairil Anuar INA, Banerjee A, Keeble AH, Carella A, Nikov GI, Howarth M
Nat Commun. 2019 Apr 15;10(1):1734. doi: 10.1038/s41467-019-09678-w. PubMed Article
Khairil Anuar INA, Banerjee A, Keeble AH, Carella A, Nikov GI, Howarth M
Nat Commun. 2019 Apr 15;10(1):1734. doi: 10.1038/s41467-019-09678-w. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 124618 | SpyDock | Expresses SpyDock to bind SpyTag non-covalently for affinity purification |
| 124661 | SpyCatcher002-oDi | Expresses SpyCatcher002-oDi for dimerization of SpyTag-proteins via dimeric coiled-coil |
| 124662 | SpyCatcher002-oTri | Expresses SpyCatcher002-oTri for trimerization of SpyTag-proteins via trimeric coiled-coil |
| 124663 | SpyCatcher002-oTet | Expresses SpyCatcher002-oTet for tetramerization of SpyTag-proteins via tetrameric coiled-coil |
| 124664 | SpyCatcher002-oPent | Expresses SpyCatcher002-oPent for pentamerization of SpyTag-proteins via pentameric coiled-coil |
| 124670 | SpyCatcher002-oHex | Expresses SpyCatcher002-oHex for hexamerization of SpyTag-proteins via hexameric coiled-coil |
| 124671 | SpyCatcher002-oHept | Expresses SpyCatcher002-oHept for heptamerization of SpyTag-proteins via heptameric coiled-coil |