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Spy&Go purification of SpyTag-proteins using pseudo-SpyCatcher to access an oligomerization toolbox.

Khairil Anuar INA, Banerjee A, Keeble AH, Carella A, Nikov GI, Howarth M
Nat Commun. 2019 Apr 15;10(1):1734. doi: 10.1038/s41467-019-09678-w. (Link opens in a new window) PubMed (Link opens in a new window) Article

Plasmids from Article

ID Plasmid Purpose
124618SpyDockExpresses SpyDock to bind SpyTag non-covalently for affinity purification
124661SpyCatcher002-oDiExpresses SpyCatcher002-oDi for dimerization of SpyTag-proteins via dimeric coiled-coil
124662SpyCatcher002-oTriExpresses SpyCatcher002-oTri for trimerization of SpyTag-proteins via trimeric coiled-coil
124663SpyCatcher002-oTetExpresses SpyCatcher002-oTet for tetramerization of SpyTag-proteins via tetrameric coiled-coil
124664SpyCatcher002-oPentExpresses SpyCatcher002-oPent for pentamerization of SpyTag-proteins via pentameric coiled-coil
124670SpyCatcher002-oHexExpresses SpyCatcher002-oHex for hexamerization of SpyTag-proteins via hexameric coiled-coil
124671SpyCatcher002-oHeptExpresses SpyCatcher002-oHept for heptamerization of SpyTag-proteins via heptameric coiled-coil

Antibodies from Article