Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

SpyCatcher002-oDi
(Plasmid #124661)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 124661 Standard format: Plasmid sent in bacteria as agar stab 1 $85

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pDEST14
  • Backbone size w/o insert (bp) 3648
  • Total vector size (bp) 4230
  • Vector type
    Bacterial Expression

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
    37°C
  • Growth Strain(s)
    DH5alpha
  • Copy number
    High Copy

Gene/Insert

  • Gene/Insert name
    SpyCatcher002-oDi
  • Species
    Synthetic
  • Insert Size (bp)
    582
  • Promoter T7
  • Tags / Fusion Proteins
    • His6 (N terminal on insert)
    • C-tag (C terminal on insert)
    • TEV cleavage site (N terminal on insert)

Cloning Information

Terms and Licenses

  • Academic/Nonprofit Terms
  • Industry Terms
    • Not Available to Industry
Trademarks:
  • Zeocin® is an InvivoGen trademark.
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    SpyCatcher002-oDi was a gift from Mark Howarth (Addgene plasmid # 124661 ; http://n2t.net/addgene:124661 ; RRID:Addgene_124661)
  • For your References section:

    Spy&Go purification of SpyTag-proteins using pseudo-SpyCatcher to access an oligomerization toolbox. Khairil Anuar INA, Banerjee A, Keeble AH, Carella A, Nikov GI, Howarth M. Nat Commun. 2019 Apr 15;10(1):1734. doi: 10.1038/s41467-019-09678-w. 10.1038/s41467-019-09678-w PubMed 30988307