We narrowed to 589 results for: hyper

1. psfTn5

   Plasmid

   #79107

   Purpose

   bacterial expression of hyperactive Tn5 transposase carrying E54K and L372P mutations

   Depositor

   Sten Linnarsson

   Insert

   transposase 5
   

   Tags

   His

   Expression

   Bacterial

   Mutation

   E54K and L372P

   Available Since

   March 17, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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2. pET15bHis6-pG-Tnp

   Plasmid

   #124884

   Purpose

   To transform BL21-Gold(DE3) and induced by IPTG for His-proteinG-Tnp (hyperactive mutant) fusion protein.

   Depositor

   Keji Zhao

   Insert

   protein G's C1D1C2D2C3 domains contained in the protein G gBLOCK.
   

   Expression

   Bacterial

   Promoter

   T7

   Available Since

   April 2, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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3. pET-45b(+)-Tn5

   Plasmid

   #112112

   Purpose

   Expresses hyperactive Tn5 transposase for next generation sequencing library preparation

   Depositor

   Frank Pugh

   Article

   Rossi et al Nat Commun. 2018 Jul 20;9(1):2842. doi:

   Insert

   Tn5
   

   Tags

   His6

   Expression

   Bacterial

   Mutation

   E54K E110K P242A L372P

   Available Since

   July 10, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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4. SypHer

   Plasmid

   #48250

   Purpose

   pH sensor

   Depositor

   Nicolas Demaurex

   Article

   Poburko et al J Biol Chem. 2011 Apr 1;286(13):11672-84

   Insert

   SypHer
   

   Expression

   Mammalian

   Mutation

   C199S

   Promoter

   CMV

   Available Since

   Oct. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

5. pOSV00170

   Plasmid

   #222934

   Purpose

   Constitutive GFP expression for Bacillus subtilis

   Depositor

   Ophelia Venturelli

   Article

   Cheng et al Nat Commun. 2023 Apr 10;14(1):2001. doi:

   Insert

   GFP(Sp)
   

   Use

   Synthetic Biology

   Expression

   Bacterial

   Promoter

   Phyperspank

   Available Since

   Sept. 20, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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6. SypHer mt

   Plasmid

   #48251

   Purpose

   pH sensor, targeted to mitochondria

   Depositor

   Nicolas Demaurex

   Article

   Poburko et al J Biol Chem. 2011 Apr 1;286(13):11672-84

   Insert

   SypHer
   

   Tags

   2 mitochondrial targeting sequences

   Expression

   Mammalian

   Mutation

   C199S

   Promoter

   CMV

   Available Since

   Oct. 28, 2013

   Availability

   Academic Institutions and Nonprofits only

   

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7. VR122 pETv2 10His-pG-Tn5 (E54K, L372P)

   Plasmid

   #198467

   Purpose

   E. coli protein expression plasmid encoding hyperactive Tn5 transposase fused to protein G, in DH5α cloning strain.

   Depositor

   Viviana Risca

   Article

   Soroczynski et al bioRxiv 2024.07.11.602973

   Insert

   10His-pG-Tn5 (E54K, L372P)
   

   Tags

   10His-pG

   Expression

   Bacterial

   Mutation

   E54K, L372P

   Promoter

   Consensus φ10 T7 Promoter

   Available Since

   April 14, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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8. pET15bHis6Tnp

   Plasmid

   #79807

   Purpose

   Transform BL21 Gold(DE3) to induce the expression of HisTnp hyperactive mutations

   Depositor

   Keji Zhao

   Article

   Lai et al Nat Methods. 2018 Sep;15(9):741-747. doi

   Insert

   His6Tnp
   

   Tags

   His

   Expression

   Bacterial

   Available Since

   Oct. 31, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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9. pOSV00157

   Plasmid

   #222937

   Purpose

   Kill switch plasmid for DNA sensor construction using Bacillus subtilis

   Depositor

   Ophelia Venturelli

   Article

   Cheng et al Nat Commun. 2023 Apr 10;14(1):2001. doi:

   Insert

   TxpA-RatA toxin-antitoxin
   

   Expression

   Bacterial

   Promoter

   Phyperspank

   Available Since

   Dec. 10, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. VR124 pETv2 10His-pG-Tn5 (E54K, L372P)

    Plasmid

    #198468

    Purpose

    E. coli protein expression plasmid encoding hyperactive Tn5 transposase fused to protein G, in T7 Express lysY/Iq protein expression strain.

    Depositor

    Viviana Risca

    Article

    Soroczynski et al bioRxiv 2024.07.11.602973

    Insert

    10His-pG-Tn5 (E54K, L372P)
    

    Tags

    10His-pG

    Expression

    Bacterial

    Mutation

    E54K, L372P

    Promoter

    Consensus φ10 T7 Promoter

    Available Since

    April 14, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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11. pA-His6-Tn5

    Plasmid

    #171871

    Purpose

    Expresses protein A and Tn5 Transposase fusion protein in bacterial cells

    Depositor

    Kaiwei Liang

    Article

    Wang et al Sci Adv. 2021 Feb 17;7(8). pii: 7/8/eabe

    Insert

    Protein A and hyperactive Tn5 transposase
    

    Tags

    His-tag

    Expression

    Bacterial

    Mutation

    WT

    Available Since

    July 27, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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12. MTK1_008

    Plasmid

    #123671

    Purpose

    Encodes ConLS and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    Connector LS-2xHS4
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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13. MTK1_009

    Plasmid

    #123672

    Purpose

    Encodes ConL1 and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    Connector L1-2xHS4
    

    Expression

    Mammalian

    Available Since

    Feb. 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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14. MTK1_013

    Plasmid

    #123676

    Purpose

    Encodes ConL5 and chicken hypersensitivity site 4 insulator as a Type 1 part to be used in the MTK system

    Depositor

    Hana El-Samad

    Article

    Fonseca et al ACS Synth Biol. 2019 Nov 15;8(11):2593-2

    Insert

    Connector L5-2xHS4
    

    Expression

    Mammalian

    Available Since

    Dec. 13, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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15. pET15bpATnp

    Plasmid

    #121137

    Purpose

    This vector encode a His6-tagged proteinA-Tnp(hyperactive form) fusion protein.

    Depositor

    Keji Zhao

    Article

    Carter et al Nat Commun. 2019 Aug 20;10(1):3747. doi:

    Insert

    Protein A-Tnp
    

    Tags

    his

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    March 8, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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16. pCMV-hyPE2

    Plasmid

    #177173

    Purpose

    Expresses hyPE2 in mammalian cells

    Depositor

    Hyongbum Kim

    Article

    Song et al Nat Commun. 2021 Sep 23;12(1):5617. doi:

    Insert

    hyperPE2
    

    Use

    CRISPR

    Tags

    SV40 NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Nov. 17, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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17. pDV17_pCMV-MCP-ADAR2dd(E488Q)-NES

    Plasmid

    #200406

    Purpose

    Expresses a compact, engineered version of ADAR2 constitutively in mammalian cells

    Depositor

    James Collins

    Article

    Gayet et al Nat Commun. 2023 Mar 11;14(1):1339. doi:

    Insert

    MCP-ADAR2dd(E488Q)-NES
    

    Use

    Synthetic Biology

    Expression

    Mammalian

    Mutation

    E488Q (hyperactive)

    Promoter

    CMV

    Available Since

    June 30, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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18. OSCA1.2-peGFP-N1

    Plasmid

    #228585

    Purpose

    Expresses OSCA1.2-eGFP in mammalian cells

    Depositor

    Huanghe Yang

    Article

    Lowry et al bioRxiv [Preprint]. 2024 Sep 3:2024.02.0

    Insert

    hyperosmolality-gated Ca2+ permeable channel (OSCA) 1.2 (AT4G22120 Mustard Weed)
    

    Expression

    Mammalian

    Available Since

    Dec. 2, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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19. pBAD-FOLD6

    Plasmid

    #186547

    Purpose

    FOLD6 "hyperfolder" green fluorescent protein: bacterial expression plasmid

    Depositor

    Gregory Petsko

    Article

    Campbell et al Nat Methods. 2022 Nov 7. pii: 10.1038/s4

    Insert

    FOLD6
    

    Tags

    6xHis

    Expression

    Bacterial

    Mutation

    mGreenLantern-C48S/V68L/C70V/K101E/T105Y/S147P/K1…

    Promoter

    araB

    Available Since

    Nov. 8, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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20. pSLQ5135: pCAG-BFP-GuideArray

    Plasmid

    #210606

    Purpose

    Guide array for hyperdCas12a-based transcriptional activator with AAAT synSeparator

    Depositor

    Stanley Qi

    Article

    Liu et al Nat Commun. 2023 Oct 18;14(1):6575. doi:

    Insert

    pCAG-BFP-GuideArray
    

    Use

    CRISPR

    Promoter

    CAG

    Available Since

    Dec. 4, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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