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Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

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172,556 results

Showing: 481 - 500 of 172556 results

  1. Venus-tBid-4E-pEGFP-C1

    Plasmid
    #166743
    Purpose
    Express Venus fused to the N-terminus of the Bcl-2 family protein, tBid with mutation in BH3 domain to disrupt binding anti-apoptotic proteins
    Depositor
    David Andrews
    Article
    Osterlund et al Sci Adv. 2022 Apr 22;8(16):eabm7375. doi
    Insert
    tBid-4E (Bid Mouse)
    Tags
    Venus
    Expression
    Mammalian
    Mutation
    4 hydrophobic residues in the BH3 region mutated …
    Promoter
    CMV
    Available Since
    March 30, 2021
    Availability
    Academic Institutions and Nonprofits only
    166743-plasmid-map-sequence-id-329004

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  5. CHRM5-DuET

    Plasmid
    #213211
    Purpose
    The DuET construct encodes the named human GPCR engineered with a cleaved hemagglutinin signal peptide, a 5' FLAG and a 3' 1D4 epitope tag optimized for expression in mammalian cells and proteomics studies.
    Depositor
    Thomas Sakmar
    Article
    Dahl et al Sci Adv. 2023 May 3;9(18):eadf9297. doi:
    Insert
    CHRM5 (CHRM5 Human)
    Tags
    1D4 and FLAG
    Expression
    Mammalian
    Promoter
    CMV, T7
    Available Since
    April 23, 2024
    Availability
    Academic Institutions and Nonprofits only
    213211-plasmid-map-sequence-id-430773

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  8. mRuby-Mito-7

    Plasmid
    #55874
    Purpose
    Localization: Mitochondria, Excitation: 558, Emission: 605
    Depositor
    Michael Davidson
    Insert
    Mito (COX8A Human)
    Tags
    mRuby
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 12, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits

  12. mCrimson2-H2B-N-22

    Plasmid
    #56191
    Purpose
    Localization: Nucleus/Histones, Excitation: 588, Emission: 615
    Depositor
    Michael Davidson
    Insert
    H2B (H2BC11 Human)
    Tags
    mCrimson2
    Expression
    Mammalian
    Mutation
    D26G and V119I in H2B
    Promoter
    CMV
    Available Since
    March 12, 2015
    Availability
    Industry, Academic Institutions, and Nonprofits
    56191-plasmid-map-sequence-id-174992

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  14. AT1R-HA

    Plasmid
    #240614
    Purpose
    Encodes AT1R, a key receptor on RAAS that regulated blood pressure
    Depositor
    Zaid Abassi
    Insert
    Angiotensin II Type 1 Receptor (AGTR1 Human)
    Tags
    HA tag
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    Aug. 19, 2025
    Availability
    Academic Institutions and Nonprofits only
    240614-plasmid-map-sequence-id-483856

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  16. pp-CBBC-Fluc

    Plasmid
    #232920
    Purpose
    Allows the Golden Gate (BsmBI) cloning of pegRNA between two Csy4-hairpins in the intron of a CMV-FLUC encoding plasmid (POL II): ipegRNAs must be processed by Csy4 in VLP- producer cells.
    Depositor
    Philippe Mangeot
    Article
    Halegua et al Nat Commun. 2025 Jan 4;16(1):397. doi: 1
    Type
    Empty backbone
    Use
    CRISPR
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    March 11, 2025
    Availability
    Academic Institutions and Nonprofits only
    232920-plasmid-map-sequence-id-464557

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  19. pDM043

    Plasmid
    #216813
    Purpose
    Aspergillus nidulans codon-adjusted mScarlet3 fluorescent protein, includes linker for N-terminal tagging.
    Depositor
    Ken Sawin
    Article
    Modaffari et al Wellcome Open Res. 2024 Oct 17;9:602. do
    Insert
    mScarlet3
    Tags
    VinnyLinker
    Expression
    Bacterial
    Mutation
    Aspergillus nidulans codon-adjusted
    Available Since
    Sept. 5, 2024
    Availability
    Academic Institutions and Nonprofits only
    216813-plasmid-map-sequence-id-430146

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Showing: 481 - 500 of 172556 results