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Plasmid#144182PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertMXI1 (MXI1 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceAug. 14, 2023AvailabilityAcademic Institutions and Nonprofits only -
pAcGFP1-N1-SialT
Plasmid#87324PurposeTo express SialT-AcGFP1 in mammalian cells, SialT encodes the full length coding sequence of β-galactosamide α-2,6-sialyltransferaseDepositorInsertST 6 beta-galactoside alpha-2,6-sialyltransferase 1 (ST6GAL1 Human)
UseTagsAcGFP1ExpressionMammalianMutationPromoterCMVAvailable sinceMay 4, 2017AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/Flag-FLJ10081
Plasmid#64775PurposeMammalian expression of Flag-tagged FLJ10081 (NSL3)DepositorInsertFLJ10081 (KANSL3 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceMay 19, 2015AvailabilityAcademic Institutions and Nonprofits only -
pACM4
Plasmid#49797PurposeePathBrick vector for pathway engineering in E. coli.DepositorTypeEmpty backboneUseTagsS-tagExpressionBacterialMutationPromoterT7Available sinceJan. 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
PX458 V3
Plasmid#226957PurposeCBh-SpCas9-2A-GFP, and hU6-sgRNA (Sp) with a BbsI golden gate cloning backbone for sgRNA. sgRNA uses 3TC scaffold for increased sgRNA expression.DepositorTypeEmpty backboneUseCRISPRTagsExpressionMammalianMutationPromoterAvailable sinceOct. 30, 2024AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hDlx-Flex-dTomato-Fishell_7 (AAV1)
Viral Prep#83894-AAV1PurposeReady-to-use AAV1 particles produced from pAAV-hDlx-Flex-dTomato-Fishell_7 (#83894). In addition to the viral particles, you will also receive purified pAAV-hDlx-Flex-dTomato-Fishell_7 plasmid DNA. hDlx-driven, Cre-dependent expression of dTomato. These AAV preparations are suitable purity for injection into animals.DepositorPromoterhDlxTagsdTomato (Cre-dependent)Available sinceAug. 28, 2020AvailabilityAcademic Institutions and Nonprofits only -
G protein alpha-s-GFP
Plasmid#66083PurposeG protein alpha-s internally tagged with EGFP and EE epitopeDepositorInsertG-alpha-s-EE-EGFP (Gnas Rat)
UseTagsEGFP flanked by SGGGS on each side was inserted i…ExpressionMammalianMutationBamHI sites in the alpha-s cDNA were removed and …PromoterCMVAvailable sinceAug. 12, 2015AvailabilityAcademic Institutions and Nonprofits only -
pCLXSN(GFP)-mTRAF2
Plasmid#174736PurposeGene overexpressionDepositorInsertTRAF2 (Traf2 Mouse)
UseRetroviralTagsExpressionMutationWTPromoterAvailable sinceSept. 20, 2021AvailabilityAcademic Institutions and Nonprofits only -
pCAG-Scarlet-Kif5a
Plasmid#118745PurposeExpresses scarlet tagged Kif5aDepositorInsertKif5a cDNA (Kif5a Mouse)
UseTagsmScarletExpressionMammalianMutationPromoterCAGAvailable sinceNov. 27, 2018AvailabilityAcademic Institutions and Nonprofits only -
pCDNA4.TO-K5-2xCSTREP
Plasmid#136154PurposeExpresses C-terminally strep tagged Kaposi's Sarcoma Associated Herpesvirus (KSHV) K5DepositorInsertK5
UseTagsExpressionMammalianMutationPromoterCMVAvailable sinceApril 17, 2020AvailabilityAcademic Institutions and Nonprofits only -
pcDNA5FRT/Flag-LOC339287(MSL1)
Plasmid#29762DepositorInsertMSL1 (MSL1 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSYN-bArrestin2-TevC-P2A-TdTomato-WPRE-bGHpA
Plasmid#89873Purposeexpresses iTango bArrestin2-TevC component and TdTomato bicistronically for AAV productionDepositorInsertbArrestin2-TevC-P2A-TdTomato
UseAAVTagsTdTomatoExpressionMammalianMutationR173H in TdTomato (Please see depositor comments …PromoterHuman synapsinAvailable sinceMay 26, 2017AvailabilityAcademic Institutions and Nonprofits only -
pUb FLAG-Human IntS6
Plasmid#198408PurposeExpresses FLAG-tagged human IntS6DepositorInsertDDX26 (INTS6 Human)
UseTags3x FLAGExpressionInsectMutationPromoterUbi-p63eAvailable sinceNov. 29, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMito-LSSmOrange
Plasmid#37135DepositorInsertLSSmOrange
UseTagshuman cytochrome C oxidase subunit VIIIExpressionMammalianMutationA44V/F84L/W145M/I165D/M167L/G202D compared to mOr…PromoterCMVAvailable sinceJune 5, 2012AvailabilityAcademic Institutions and Nonprofits only -
pFH2.155_LV-Puro-T2A-mTagBFP2
Plasmid#205553PurposeExpress puromycin resistance marker and mTagBFP2DepositorInsertPuro-T2A-mTagBFP2
UseLentiviralTagsExpressionMammalianMutationWTPromoterEF1Available sinceSept. 26, 2023AvailabilityAcademic Institutions and Nonprofits only -
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pcDNA5FRT/Flag-FLJ20436
Plasmid#29757DepositorInsertFLJ20436 (C12orf41 Human)
UseTagsFlagExpressionMammalianMutationPromoterAvailable sinceNov. 15, 2013AvailabilityAcademic Institutions and Nonprofits only -
pYESir2HY-puro
Plasmid#1770DepositorInsertSir2 (SIRT1 Human)
UseRetroviralTagsExpressionMammalianMutationH363Y, residue 363 changed from histidine to tyro…PromoterAvailable sinceJune 3, 2005AvailabilityAcademic Institutions and Nonprofits only -
pICH47742:FCP:Cas9YFP
Plasmid#85986PurposeGolden Gate Level 1 cassette encoding a Cas9-YFP fusion under the FCP promoterDepositorInsertCas9
UseCRISPR; Golden gate assemblyTagsSV40 NLS, YFPExpressionMutationPromoterFCPAvailable sinceJan. 18, 2017AvailabilityAcademic Institutions and Nonprofits only -
Epac-S-H81
Plasmid#170339PurposeGFPDel(UST)_EPACdDEPCD_mRFP biosensor to measure realtime changes in FRET reflecting fluctuations in cAMP levels in living cells.DepositorInsertGFPDel(UST)_EPACdDEPCD_mRFP (RAPGEF3 Human)
UseTagsExpressionMammalianMutationDeletion of DEP domain, Catalytically dead T781A …PromoterCMVAvailable sinceOct. 5, 2021AvailabilityAcademic Institutions and Nonprofits only