169,069 results

1. pcDNA3-GFP-LC3-RFP-LC3ΔG

   Plasmid

   #168997

   Purpose

   Expresses GFP-LC3-RFP-LC3ΔG in mammalian cells and zebrafish to measure autophagic flux

   Depositor

   Noboru Mizushima

   Article

   Kaizuka et al Mol Cell. 2016 Nov 17;64(4):835-849. doi

   Insert

   microtubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
   

   Tags

   EGFP and mRFP1

   Expression

   Mammalian

   Available Since

   April 26, 2021

   Availability

   Academic Institutions and Nonprofits only

   

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2. TFORF2971

   Plasmid

   #144447

   Purpose

   Lentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.

   Depositor

   Feng Zhang

   Article

   Joung et al Cell. 2023 Jan 5;186(1):209-229.e26. doi

   Insert

   NR1H4 (NR1H4 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mammalian

   Promoter

   EF1a

   Available Since

   April 27, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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3. Human Epigenetic Knockout Library

   Pooled Library

   #162256

   Purpose

   Knockout library that targets human epigenetic genes.

   Depositor

   Kivanc Birsoy

   Article

   Williams et al Cell Metab. 2020 Apr 7;31(4):852-861.e6.

   Expression

   Mammalian

   Species

   Homo sapiens

   Use

   CRISPR and Lentiviral

   Available Since

   Nov. 17, 2020

   Availability

   Academic Institutions and Nonprofits only

4. pAAV-CaMKIIa-EGFP (AAV8)

   Viral Prep

   #50469-AAV8

   Purpose

   Ready-to-use AAV8 particles produced from pAAV-CaMKIIa-EGFP (#50469). In addition to the viral particles, you will also receive purified pAAV-CaMKIIa-EGFP plasmid DNA. CamKIIa-driven EGFP expression control. These AAV preparations are suitable purity for injection into animals.

   Depositor

   Bryan Roth

   Promoter

   CaMKII

   Tags

   EGFP

   Available Since

   Nov. 8, 2016

   Availability

   Academic Institutions and Nonprofits only

5. pRK5_mCherry_P2AT2A_GFP-nb-2xKS_FtoG

   Plasmid

   #238237

   Purpose

   For overexpression of mCherry_P2AT2A_GFP-nb-2xKS_FtoG

   Depositor

   Denes Hnisz

   Article

   Zhang et al Nature. 2025 Jun 4. doi: 10.1038/s41586-

   Insert

   mCherry_P2AT2A_GFP-nb-2xKS_FtoG
   

   Expression

   Mammalian

   Available Since

   June 3, 2025

   Availability

   Academic Institutions and Nonprofits only

   

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6. pDisplay-GRAB_SST1.0

   Plasmid

   #208664

   Purpose

   Expresses the genetically-encoded fluorescent somatostatin (SST) sensor GRAB_SST1.0 in mammalian cells

   Depositor

   Yulong Li

   Article

   Wang et al Science. 2023 Nov 17;382(6672):eabq8173.

   Insert

   GPCR activation based somatostatin (SST) sensor GRAB_SST1.0
   

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Jan. 16, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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7. pC0046-EF1a-PspCas13b-NES-HIV

   Plasmid

   #103862

   Purpose

   Expresses PspCas13b in mammalian cells for knockdown of target RNAs in combination with compatible guide RNAs. Localized to the cytoplasm

   Depositor

   Feng Zhang

   Article

   Cox et al Science. 2017 Oct 25. pii: eaaq0180. doi

   Insert

   PspCas13b
   

   Use

   CRISPR and Lentiviral

   Tags

   3xHA and HIV NES

   Expression

   Mammalian

   Available Since

   Nov. 28, 2017

   Availability

   Industry, Academic Institutions, and Nonprofits

   

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8. pAAV-hsyn-NE2h

   Plasmid

   #208687

   Purpose

   Expresses the genetically-encoded fluorescent norepinephrine (NE) sensor GRAB_NE2h in neurons

   Depositor

   Yulong Li

   Article

   Feng et al Neuron. 2024 Jun 19;112(12):1930-1942.e6

   Has Service

   AAV5

   Insert

   GPCR activation based norepinephrine (NE) sensor GRAB_NE2h
   

   Use

   AAV

   Promoter

   hSyn

   Available Since

   Oct. 24, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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9. CaV1.2/pcDNA3.1~ Human

   Plasmid

   #140576

   Purpose

   Expression in mammallian cells or oocytes

   Depositor

   Dan Minor

   Article

   Abderemane-Ali et al Neuron. 2019 Mar 20;101(6):1134-1149.e3.

   Insert

   human CaV1.2 (CACNA1C Human)
   

   Expression

   Mammalian

   Promoter

   CMV and T7

   Available Since

   April 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

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10. pCMV-PE7-SB2

    Plasmid

    #228868

    Purpose

    PE7 connected to MLH1-SB and MLH1-SB-NLS via 2A sequence

    Depositor

    Sangsu Bae

    Article

    Park et al Cell. 2025 Aug 1:S0092-8674(25)00799-8.

    Insert

    PE7-2A-MLH1-SB-NLS-2A-MLH1-SB
    

    Use

    CRISPR

    Available Since

    Dec. 16, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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11. Lamp1-mNeonGreen

    Plasmid

    #98882

    Purpose

    In vivo visualization of lysosomes (can be used for colocalization studies)

    Depositor

    Dorus Gadella

    Article

    Chertkova et al bioRxiv (2020) 160374; doi: https://doi.

    Insert

    Lamp1
    

    Tags

    mNeonGreen

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    March 7, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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12. AAVS1-TALEN-L

    Plasmid

    #59025

    Purpose

    TALEN designed to target the human AAVS1/PPP1R12C locus

    Depositor

    Danwei Huangfu

    Article

    Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

    Insert

    AAVS1-TALEN-L
    

    Use

    AAV and TALEN

    Tags

    3xFlag and NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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13. AAVS1-TALEN-R

    Plasmid

    #59026

    Purpose

    TALEN designed to target the human AAVS1/PPP1R12C locus

    Depositor

    Danwei Huangfu

    Article

    Gonzalez et al Cell Stem Cell. 2014 Jun 11. pii: S1934-

    Insert

    AAVS1-TALEN-R
    

    Use

    AAV and TALEN

    Tags

    3xFlag and NLS

    Expression

    Mammalian

    Promoter

    CMV

    Available Since

    Aug. 12, 2014

    Availability

    Academic Institutions and Nonprofits only

    

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14. pAAV-Syn-ChrimsonR-tdT (AAV9)

    Viral Prep

    #59171-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pAAV-Syn-ChrimsonR-tdT (#59171). In addition to the viral particles, you will also receive purified pAAV-Syn-ChrimsonR-tdT plasmid DNA. Syn-driven ChrimsonR-tdTomato expression for optogenetic neural activation. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Edward Boyden

    Article

    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:

    Promoter

    Syn

    Tags

    tdTomato

    Available Since

    Feb. 7, 2018

    Availability

    Academic Institutions and Nonprofits only

15. pET21b FAST-PETase

    Plasmid

    #225760

    Purpose

    Expresses codon-optimized FAST-PETase in E. coli with a C-terminal his-tag

    Depositor

    Jason Boock

    Article

    Carter et al Microb Cell Fact. 2024 Nov 25;23(1):319.

    Insert

    FAST-PETase
    

    Tags

    6xHis Tag

    Expression

    Bacterial

    Promoter

    T7

    Available Since

    Dec. 11, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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16. pmCherryC1 VAP-A

    Plasmid

    #226407

    Purpose

    Expression of human VAP-A fused to mCherry in mammalian cells

    Depositor

    Catherine Tomasetto

    Article

    Di Mattia et al EMBO Rep. 2018 Jul;19(7). pii: embr.2017

    Insert

    VAP-A (VAPA Human)
    

    Tags

    T7-Xpress tag and mCherry

    Expression

    Mammalian

    Available Since

    Oct. 4, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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17. pAAVS1-NDi-CRISPRi (Gen2)

    Plasmid

    #73498

    Purpose

    Dox-inducible CRISPR interference (CRISPRi) knock in construct into the AAVS1 locus

    Depositor

    Bruce Conklin

    Article

    Mandegar et al Cell Stem Cell. 2016 Apr 7;18(4):541-53.

    Inserts

    dCas9-KRAB
    rtTA
    

    Use

    CRISPR

    Tags

    HA, KRAB, and NLS

    Expression

    Mammalian

    Mutation

    D10A, H840A (catalytically deactivated Cas9)

    Promoter

    CAG and TRE3G

    Available Since

    Dec. 7, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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18. pT4/HB

    Plasmid

    #108352

    Purpose

    Optimized Sleeping Beauty Transposon Vector with MCS

    Depositor

    Wolfgang Uckert

    Article

    Wang et al Nucleic Acids Res. 2017 Jan 9;45(1):311-

    Type

    Empty backbone

    Use

    Transposon

    Available Since

    June 25, 2018

    Availability

    Academic Institutions and Nonprofits only

    

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19. p101_CRISPRai_PiggyBac

    Plasmid

    #213777

    Purpose

    Expresses Tet-On inducible CRISPRai orthogonal system with VPR-dSaCas9, dSpCas9-KRAB-BFP, zeocin resistance gene, and Tet-On transactivator. PiggyBac vector.

    Depositor

    Howard Chang

    Article

    Pacalin et al Nat Biotechnol. 2024 May 17. doi: 10.103

    Inserts

    VPR-dSaCas9
    dSpCas9-KRAB-BFP
    zeocin
    Tet-On transactivator
    

    Use

    CRISPR

    Promoter

    EF1a (EF-1-alpha intron a) and Tet_On_TRE

    Available Since

    May 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. SB100X in pCAG globin pA

    Plasmid

    #127909

    Purpose

    Sleeping Beauty Transposase expression vector driven by the CAG promoter

    Depositor

    Mark Groudine

    Article

    Strom et al Elife. 2021 Jun 9;10. pii: 63972. doi: 1

    Insert

    Sleeping Beauty Transposase
    

    Expression

    Mammalian

    Available Since

    Sept. 12, 2019

    Availability

    Academic Institutions and Nonprofits only

    

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