Ef1a_Large T-antigen_Ires_Puro
(Plasmid #18922)

• Depositing Lab: Linzhao Cheng
• Publication: Mali et al Stem Cells. 2008 Aug . 26(8):1998-2005.

Backbone

• Vector backbone: EGIP
• Backbone size w/o insert (bp): 10000
• Vector type: Mammalian Expression, Lentiviral, Cre/Lox
• Selectable markers: Puromycin

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): Stbl3
• Growth instructions: Stbl3
• Copy number: Unknown

Gene/Insert

• Gene/Insert name: Large T Antigen
• Species: Simian virus 40
• Insert Size (bp): 2200
• GenBank ID: NC_001669
• Entrez Gene: SV40gp6 (a.k.a. SV40gp6)

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: EcoRI (not destroyed)
• 3′ cloning site: EcoRI (not destroyed)
• 5′ sequencing primer: Ef1a

Resource Information

• Addgene Notes:
  • Addgene diagnostic digest
• Articles Citing this Plasmid:
  • 10 References

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry