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pRRLsin-SV40 T antigen-IRES-mCherry
(Plasmid #58993)

Ordering

Item Catalog # Description Quantity Price (USD)
Plasmid 58993 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

  • Vector backbone
    pRRLsin.cPPT.PGK/GFP.WPRE
  • Backbone size w/o insert (bp) 6110
  • Total vector size (bp) 11405
  • Modifications to backbone
    NotI site at nt 924 was eliminated
  • Vector type
    Mammalian Expression, Lentiviral
  • Selectable markers
    mCherry red fluorescence

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    30°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    High Copy

Gene/Insert 1

  • Gene/Insert name
    CMV enhancer-chicken beta actin promoter
  • Insert Size (bp)
    1730
  • Mutation
    EcoRV site in MCS converted to BamHI site

Cloning Information for Gene/Insert 1

  • Cloning method Restriction Enzyme
  • 5′ cloning site SalI (destroyed during cloning)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer none
  • 3′ sequencing primer pCAG-F
  • (Common Sequencing Primers)

Gene/Insert 2

  • Gene/Insert name
    SV40 large T antigen
  • Alt name
    SV40gp6
  • Insert Size (bp)
    2200
  • Promoter CMV enhancer-chicken beta actin

Cloning Information for Gene/Insert 2

  • Cloning method Restriction Enzyme
  • 5′ cloning site BamHI (not destroyed)
  • 3′ cloning site BamHI (not destroyed)
  • 5′ sequencing primer pCAG-F
  • 3′ sequencing primer IRES-R
  • (Common Sequencing Primers)

Gene/Insert 3

  • Gene/Insert name
    IRES-mCherry red fluorescent protein
  • Species
    Synthetic
  • Insert Size (bp)
    1360
  • Promoter CMV enhancer-chicken beta actin

Cloning Information for Gene/Insert 3

  • Cloning method Restriction Enzyme
  • 5′ cloning site NotI (not destroyed)
  • 3′ cloning site NotI (not destroyed)
  • 5′ sequencing primer IRES-R
  • 3′ sequencing primer mCherry-F
  • (Common Sequencing Primers)

Resource Information

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pRRLsin-SV40 T antigen-IRES-mCherry was a gift from Snorri Thorgeirsson (Addgene plasmid # 58993 ; http://n2t.net/addgene:58993 ; RRID:Addgene_58993)
  • For your References section:

    Modeling pathogenesis of primary liver cancer in lineage-specific mouse cell types. Holczbauer A, Factor VM, Andersen JB, Marquardt JU, Kleiner DE, Raggi C, Kitade M, Seo D, Akita H, Durkin ME, Thorgeirsson SS. Gastroenterology. 2013 Jul;145(1):221-31. doi: 10.1053/j.gastro.2013.03.013. Epub 2013 Mar 19. 10.1053/j.gastro.2013.03.013 PubMed 23523670