Ef1a_Large T-antigen_Ires_Puro Notes
Addgene diagnostic digest
This gel depicts the NEB 1kb ladder and a digestion of the plasmid with the following enzyme: EcoRI.
We expected the following band sizes for this digest: 10kb and 2.2kb. We see the expected insert size (2.2kb). The vector backbone appears to be slightly smaller than the reported 10kb.
