233,069 results

1. CARPID dCasRx-BASU

   Plasmid

   #153303

   Purpose

   express CARPID dCasRx-BASU fusion protein in mammalian cells

   Depositor

   Jian Yan, Liang Zhang

   Article

   Yi et al Nat Methods. 2020 Jun 22. pii: 10.1038/s

   Insert

   dCasRx-BASU
   

   Use

   CRISPR and Lentiviral

   Expression

   Mammalian

   Mutation

   R239A/H244A/R858A/H863A in RfxCas13d

   Promoter

   EF-1a

   Available Since

   June 29, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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2. pAAVS1-TRE-Cas9- puro-polyA-CAG-rtTA

   Plasmid

   #107270

   Purpose

   Expresses Cas9 upon induction with doxicycline.

   Depositor

   Pablo Menéndez

   Article

   Castano et al Stem Cell Res. 2017 May;21:137-140. doi:

   Inserts

   hSpCas9
   rtTA
   bGH polyA-SV40polyA
   

   Tags

   3xFlag, Nucleoplasmin NLS, and SV40 NLS

   Expression

   Mammalian

   Promoter

   CAG and TRE

   Available Since

   April 19, 2018

   Availability

   Academic Institutions and Nonprofits only

   

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3. pTRE/VEGF/IRES/EGFP

   Plasmid

   #206211

   Purpose

   An expression vector of VEGF and EGFP under control of TRE promoter

   Depositor

   Masamichi Kamihira

   Article

   Masumoto et al J Biosci Bioeng. 2021 Oct;132(4):399-407

   Insert

   vascular endothelial growth factor (Vegfa Mouse)
   

   Use

   Lentiviral

   Expression

   Mammalian

   Available Since

   Oct. 4, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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4. pKL2351

   Plasmid

   #199721

   Purpose

   A pVS1-based T-DNA binary vector and destination vector for Gateway cloning.

   Depositor

   Kan Wang

   Article

   Lee et al Plant Physiol. 2023 Apr 18:kiad231. doi:

   Insert

   NPTII
   

   Expression

   Plant

   Available Since

   July 5, 2023

   Availability

   Academic Institutions and Nonprofits only

   

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5. pCB578

   Plasmid

   #141095

   Purpose

   E. coli-Lactobacilli shuttle vector containing SpCas9, tracrRNA, and a repeat-spacer-repeat array

   Depositor

   Chase Beisel

   Article

   Leenay et al Biotechnol J. 2019 Mar;14(3):e1700583. d

   Inserts

   Cas9 and tracrRNA
   repeat-spacer-repeat array
   

   Expression

   Bacterial

   Available Since

   April 24, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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6. pY004 (pcDNA3.1-hFnCpf1)

   Plasmid

   #69976

   Purpose

   Expresses humanized FnCpf1

   Depositor

   Feng Zhang

   Article

   Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

   Insert

   hFnCpf1
   

   Use

   CRISPR

   Tags

   NLS & 3xHA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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7. pY004 (pcDNA3.1-hFnCpf1)

   Plasmid

   #69976

   Purpose

   Expresses humanized FnCpf1

   Depositor

   Feng Zhang

   Article

   Zetsche et al Cell. 2015 Sep 23. pii: S0092-8674(15)01

   Insert

   hFnCpf1
   

   Use

   CRISPR

   Tags

   NLS & 3xHA

   Expression

   Mammalian

   Promoter

   CMV

   Available Since

   Oct. 8, 2015

   Availability

   Academic Institutions and Nonprofits only

   

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8. pXG260

   Plasmid

   #141282

   Purpose

   CMV:EGFP (as control for pXG237)

   Depositor

   Martin Kampmann

   Article

   Guo et al Nature. 2020 Mar;579(7799):427-432. doi:

   Insert

   EGFP
   

   Use

   Lentiviral

   Promoter

   CMV

   Available Since

   Sept. 19, 2020

   Availability

   Academic Institutions and Nonprofits only

   

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9. nesprin TSmod 25

   Plasmid

   #125555

   Purpose

   Nesprin-2G force sensor with cerulean-F25-venus TSmodule

   Depositor

   Daniel Conway

   Article

   Mayer et al Cell Mol Bioeng. 2019 Jul 11;12(4):289-3

   Insert

   mini-nesprin-2G (Syne2 Mouse)
   

   Tags

   cerulean, venus

   Expression

   Mammalian

   Mutation

   TSmod changed from mTFP to cerulean, flageliform …

   Promoter

   CMV

   Available Since

   July 25, 2019

   Availability

   Academic Institutions and Nonprofits only

   

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10. pMRXIP GFP-Stx17 WT

    Plasmid

    #45909

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

    Insert

    Syntaxin 17 (STX17 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Promoter

    CMV

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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11. pMRXIP GFP-Stx17 WT

    Plasmid

    #45909

    Depositor

    Noboru Mizushima

    Article

    Itakura et al Cell. 2012 Dec 7;151(6):1256-69. doi: 10

    Insert

    Syntaxin 17 (STX17 Human)
    

    Use

    Retroviral

    Tags

    GFP

    Promoter

    CMV

    Available Since

    July 10, 2013

    Availability

    Academic Institutions and Nonprofits only

    

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12. pRGEN-CMV-10XGCN4-L1-dCjCas9 S-D

    Plasmid

    #169912

    Purpose

    Expression of a SunTag-dCjCas9 fusion protein in which 10 copies of the GCN4 peptide are fused to the N terminus of dCjCas9.

    Depositor

    Zhili Rong

    Article

    Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

    Insert

    dCjCas9
    

    Use

    CRISPR

    Tags

    10XGCN4, 2xSV40NLS, and SV40NLS-HA

    Expression

    Mammalian

    Mutation

    D8A, H559A in cjCas9

    Promoter

    CMV

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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13. pBK1304-AAV-EFSNC-dCjCas9-KRAB-MECP2

    Plasmid

    #223149

    Purpose

    Expression of KRAB and truncated MECP2 with dCjCas9 and empty gRNA scaffold

    Depositor

    Boris Kantor

    Article

    Kantor et al bioRxiv [Preprint]. 2024 May 19:2023.04.

    Insert

    KRAB-MECP2 (MECP2 Human)
    

    Use

    AAV and CRISPR

    Tags

    3xFLAG

    Expression

    Mammalian

    Mutation

    Encodes aa 204-310 (MECP2)

    Promoter

    EF1a

    Available Since

    Aug. 22, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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14. pHAGE-3xGFP-tDeg

    Plasmid

    #191371

    Purpose

    Overexpression of 3xGFP-tDeg in human cells

    Depositor

    Ling-Ling Chen

    Article

    Yang et al Cell Insight. 2022 Jun 15;1(4):100044. d

    Insert

    3xGFP-tDeg
    

    Tags

    3xGFP

    Expression

    Mammalian

    Available Since

    Oct. 26, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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15. AAV:ITR-EF1aCore-SaCas9-single(U6-sgRNA(backbone))-ITR

    Plasmid

    #207880

    Purpose

    AAV vector encoding EF1a core promoter-driven SaCas9 and single sgRNA cloning site w/ the engineered Sa Guide scaffold variant (BbsI for sgRNA cloning).

    Depositor

    Yeh-Hsing Lao

    Article

    Lao et al Proc Natl Acad Sci U S A. 2023 Aug 22;12

    Type

    Empty backbone

    Use

    AAV and CRISPR

    Expression

    Mammalian

    Available Since

    Nov. 16, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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16. pRDA_336

    Plasmid

    #179095

    Purpose

    BE Cas expression

    Depositor

    John Doench, David Root

    Article

    Sangree et al Nat Commun. 2022 Mar 14;13(1):1318. doi:

    Insert

    BE3.9 (Cas9-NG)
    

    Use

    CRISPR and Lentiviral

    Available Since

    Jan. 19, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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17. ZsGreen1-cMYC/pLVX-Puromycin

    Plasmid

    #180278

    Purpose

    Plasmid encoding human c-MYC for reporter assays. Polycistronic puromycin selectable lentiviral vector (unmodified, from TAKARA BIO).

    Depositor

    Andre Catic

    Article

    Moree et al Cancers (Basel). 2022 Feb 4;14(3). pii:

    Insert

    ZsGreen1-cMYC fusion protein MYC (MYC Human)
    

    Use

    Lentiviral

    Tags

    GFP

    Expression

    Mammalian

    Available Since

    March 22, 2022

    Availability

    Academic Institutions and Nonprofits only

    

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18. pU6-cj-E sgRNA

    Plasmid

    #169915

    Purpose

    Expression of sgRNA on an optimized gRNA scaffold contains A-U pair flip stabilize the CjCas9/sgRNA complex.

    Depositor

    Zhili Rong

    Article

    Zhang et al Nucleic Acids Res. 2021 Mar 22. pii: 617

    Type

    Empty backbone

    Use

    CRISPR

    Expression

    Mammalian

    Available Since

    June 25, 2021

    Availability

    Academic Institutions and Nonprofits only

    

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19. #2.4 pre-G-SynapShot-TagBFP

    Plasmid

    #214721

    Purpose

    Expresses green #2.4 pre-SynapShot and TagBFP

    Depositor

    Won Do Heo

    Article

    Son et al Nat Methods. 2024 Jan 8. doi: 10.1038/s4

    Insert

    #2.4 pre-G-SynapShot
    

    Use

    AAV

    Tags

    TagBFP

    Expression

    Mammalian

    Promoter

    CAG

    Available Since

    April 3, 2024

    Availability

    Academic Institutions and Nonprofits only

    

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20. pENN.AAV.GFAP.iGluSnFr.WPRE.SV40 (AAV9)

    Viral Prep

    #98930-AAV9

    Purpose

    Ready-to-use AAV9 particles produced from pENN.AAV.GFAP.iGluSnFr.WPRE.SV40 (#98930). In addition to the viral particles, you will also receive purified pENN.AAV.GFAP.iGluSnFr.WPRE.SV40 plasmid DNA. GFAP-driven glutamate sensor. These AAV preparations are suitable purity for injection into animals.

    Depositor

    Loren Looger

    Article

    Marvin et al Nat Methods. 2013 Feb;10(2):162-70. doi:

    Promoter

    GFAP

    Available Since

    April 13, 2018

    Availability

    Academic Institutions and Nonprofits only