CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins.
Savic D, Partridge EC, Newberry KM, Smith SB, Meadows SK, Roberts BS, Mackiewicz M, Mendenhall EM, Myers RM
Genome Res. 2015 Sep 9. PubMed Article
Savic D, Partridge EC, Newberry KM, Smith SB, Meadows SK, Roberts BS, Mackiewicz M, Mendenhall EM, Myers RM
Genome Res. 2015 Sep 9. PubMed Article
Plasmids from Article
| ID | Plasmid | Purpose | |
|---|---|---|---|
| 63934 | pFETCh_Donor (EMM0021) | 3X Flag with P2A-Neo for 3' tagging of endogenous proteins |
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| 64036 | PX458_CEBPB_1 | Expresses gRNA against human CEBPB along with Cas9 with 2A GFP |
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| 64047 | PX458_CEBPB_2 | Expresses gRNA against human CEBPB along with Cas9 with 2A GFP |
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| 64055 | pFETCh_CEBPB | Homology Arms and 3X Flag with P2A Neo for 3' tagging of human CEBPB |
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| 64056 | pFETCh_RAD21 | Homology Arms and 3X Flag with P2A Neo for 3' tagging of human RAD21 |
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| 64057 | PX458_RAD21_1 | Expresses gRNA against human RAD21 along with Cas9 with 2A GFP |
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| 64253 | pFETCh_GABPA | Homology Arms and 3X Flag with P2A Neo for 3' tagging of human GABPA |
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| 64254 | PX458_GABPA_1 | Expresses gRNA against human GABPA along with Cas9 with 2A GFP |
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| 64255 | PX458_GABPA_2 | Expresses gRNA against human GABPA along with Cas9 with 2A GFP |
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| 64689 | pFETCH_ATF1 | Homology Arms and 3X Flag with P2A Neo for 3' tagging of human ATF1 |
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| 64690 | PX458_ATF1_1 | Expresses gRNA against human ATF1 along with Cas9 with 2A GFP |
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| 64938 | pFETCH_CREB1 | Homology Arms and 3X Flag with P2A Neo for 3' tagging of human CREB1 |
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| 64939 | PX458_CREB1_1 | Expresses gRNA against human CREB1 along with Cas9 with 2A GFP |
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| 64940 | PX458_CREB1_2 | Expresses gRNA against human CREB1 along with Cas9 with 2A GFP |
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| 86260 | pFETCh_KDM1A | Donor vector for 3' FLAG tag of human KDM1A |
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| 86261 | pFETCh_FOXP1 | Donor vector for 3' FLAG tag of human FOXP1 |
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| 86262 | pFETCh_HLF | Donor vector for 3' FLAG tag of human HLF |
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| 86263 | pFETCh_MBD1_iso1 | Donor vector for 3' FLAG tag of human MBD1_iso1 |
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| 86264 | pFETCh_ZNF644 | Donor vector for 3' FLAG tag of human ZNF644 |
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| 86265 | pFETCh_KDM6A | Donor vector for 3' FLAG tag of human KDM6A |
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| 86266 | pFETCh_KAT7 | Donor vector for 3' FLAG tag of human KAT7 |
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| 86267 | pFETCh_SAP130 | Donor vector for 3' FLAG tag of human SAP130 |
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| 86268 | pFETCh_ZSCAN9 | Donor vector for 3' FLAG tag of human ZSCAN9 |
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| 86269 | pFETCh_KLF11 | Donor vector for 3' FLAG tag of human KLF11 |
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| 86270 | pFETCh_ZNF652 | Donor vector for 3' FLAG tag of human ZNF652 |
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| 86271 | pFETCh_HMG20A | Donor vector for 3' FLAG tag of human HMG20A |
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| 86272 | pFETCh_HBP1 | Donor vector for 3' FLAG tag of human HBP1 |
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| 86274 | pFETCh_MIER3 | Donor vector for 3' FLAG tag of human MIER3 |
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| 86276 | pFETCh_ZGPAT | Donor vector for 3' FLAG tag of human ZGPAT |
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| 86277 | pFETCh_MLX | Donor vector for 3' FLAG tag of human MLX |
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| 86278 | pFETCh_ZNF580 | Donor vector for 3' FLAG tag of human ZNF580 |
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| 86279 | pFETCh_GATAD2A | Donor vector for 3' FLAG tag of human GATAD2A |
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| 86280 | pFETCh_MIXL1 | Donor vector for 3' FLAG tag of human MIXL1 |
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| 86281 | pFETCh_TEAD3 | Donor vector for 3' FLAG tag of human TEAD3 |
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| 86282 | pFETCh_ZNF792 | Donor vector for 3' FLAG tag of human ZNF792 |
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| 86283 | pFETCh_NCoA2 | Donor vector for 3' FLAG tag of human NCoA2 |
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| 86284 | pFETCh_NR2F6 | Donor vector for 3' FLAG tag of human NR2F6 |
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| 86285 | pFETCh_CEBPG | Donor vector for 3' FLAG tag of human CEBPG |
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| 86286 | pFETCh_KLF9 | Donor vector for 3' FLAG tag of human KLF9 |
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| 86287 | pFETCh_NFIL3 | Donor vector for 3' FLAG tag of human NFIL3 |
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| 86288 | pFETCh_ARID5B | Donor vector for 3' FLAG tag of human ARID5B |
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| 86289 | pFETCh_HOMEZ | Donor vector for 3' FLAG tag of human HOMEZ |
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| 86291 | pFETCh_TEAD1 | Donor vector for 3' FLAG tag of human TEAD1 |
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| 86292 | pFETCh_SOX5 | Donor vector for 3' FLAG tag of human SOX5 |
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| 86293 | pFETCh_CEBPA | Donor vector for 3' FLAG tag of human CEBPA |
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| 86294 | pFETCh_ZFP1_iso1 | Donor vector for 3' FLAG tag of human ZFP1_iso1 |
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| 86295 | pFETCh_SP5 | Donor vector for 3' FLAG tag of human SP5 |
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| 86296 | pFETCh_RFXANK | Donor vector for 3' FLAG tag of human RFXANK |
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| 86297 | PX458_KDM1A_1 | Encodes gRNA for 3' target of human KDM1A |
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| 86298 | PX458_KDM1A_2 | Encodes gRNA for 3' target of human KDM1A |
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| 86299 | PX458_FOXP1_1 | Encodes gRNA for 3' target of human FOXP1 |
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| 86300 | PX458_FOXP1_2 | Encodes gRNA for 3' target of human FOXP1 |
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| 86301 | PX458_HLF_1 | Encodes gRNA for 3' target of human HLF |
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| 86302 | PX458_HLF_2 | Encodes gRNA for 3' target of human HLF |
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| 86303 | PX458_MBD1_iso1_1 | Encodes gRNA for 3' target of human MBD1_iso1 |
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| 86304 | PX458_MBD1_iso1_2 | Encodes gRNA for 3' target of human MBD1_iso1 |
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| 86305 | PX458_ZNF644_1 | Encodes gRNA for 3' target of human ZNF644 |
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| 86306 | PX458_ZNF644_2 | Encodes gRNA for 3' target of human ZNF644 |
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| 86307 | PX458_KDM6A_1 | Encodes gRNA for 3' target of human KDM6A |
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| 86308 | PX458_KDM6A_2 | Encodes gRNA for 3' target of human KDM6A |
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| 86309 | PX458_KAT7_1 | Encodes gRNA for 3' target of human KAT7 |
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| 86310 | PX458_SAP130_1 | Encodes gRNA for 3' target of human SAP130 |
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| 86311 | PX458_SAP130_2 | Encodes gRNA for 3' target of human SAP130 |
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| 86312 | PX458_ZSCAN9_1 | Encodes gRNA for 3' target of human ZSCAN9 |
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| 86313 | PX458_ZSCAN9_2 | Encodes gRNA for 3' target of human ZSCAN9 |
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| 86314 | PX458_KLF11_1 | Encodes gRNA for 3' target of human KLF11 |
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| 86315 | PX458_KLF11_2 | Encodes gRNA for 3' target of human KLF11 |
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| 86316 | PX458_ZNF652_1 | Encodes gRNA for 3' target of human ZNF652 |
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| 86317 | PX458_ZNF652_2 | Encodes gRNA for 3' target of human ZNF652 |
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| 86318 | PX458_HMG20A_1 | Encodes gRNA for 3' target of human HMG20A |
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| 86319 | PX458_HMG20A_2 | Encodes gRNA for 3' target of human HMG20A |
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| 86320 | PX458_HBP1_1 | Encodes gRNA for 3' target of human HBP1 |
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| 86321 | PX458_KDM3A_1 | Encodes gRNA for 3' target of human KDM3A |
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| 86322 | PX458_KDM3A_2 | Encodes gRNA for 3' target of human KDM3A |
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| 86323 | PX458_MIER3_1 | Encodes gRNA for 3' target of human MIER3 |
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| 86324 | PX458_MIER3_2 | Encodes gRNA for 3' target of human MIER3 |
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| 86325 | PX458_TFE3_1 | Encodes gRNA for 3' target of human TFE3 |
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| 86326 | PX458_TFE3_2 | Encodes gRNA for 3' target of human TFE3 |
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| 86327 | PX458_ZGPAT_1 | Encodes gRNA for 3' target of human ZGPAT |
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| 86328 | PX458_ZGPAT_2 | Encodes gRNA for 3' target of human ZGPAT |
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| 86329 | PX458_MLX_1 | Encodes gRNA for 3' target of human MLX |
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| 86330 | PX458_MLX_2 | Encodes gRNA for 3' target of human MLX |
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| 86331 | PX458_ZNF580_1 | Encodes gRNA for 3' target of human ZNF580 |
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| 86332 | PX458_ZNF580_2 | Encodes gRNA for 3' target of human ZNF580 |
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| 86333 | PX458_GATAD2A_1 | Encodes gRNA for 3' target of human GATAD2A |
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| 86334 | PX458_GATAD2A_2 | Encodes gRNA for 3' target of human GATAD2A |
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| 86335 | PX458_MIXL1_1 | Encodes gRNA for 3' target of human MIXL1 |
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| 86336 | PX458_MIXL1_2 | Encodes gRNA for 3' target of human MIXL1 |
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| 86337 | PX458_TEAD3_1 | Encodes gRNA for 3' target of human TEAD3 |
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| 86338 | PX458_TEAD3_2 | Encodes gRNA for 3' target of human TEAD3 |
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| 86339 | PX458_ZNF792_1 | Encodes gRNA for 3' target of human ZNF792 |
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| 86340 | PX458_ZNF792_2 | Encodes gRNA for 3' target of human ZNF792 |
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| 86341 | PX458_NCoA2_1 | Encodes gRNA for 3' target of human NCoA2 |
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| 86342 | PX458_NR2F6_1 | Encodes gRNA for 3' target of human NR2F6 |
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| 86343 | PX458_NR2F6_2 | Encodes gRNA for 3' target of human NR2F6 |
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| 86344 | PX458_CEBPG_1 | Encodes gRNA for 3' target of human CEBPG |
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| 86345 | PX458_CEBPG_2 | Encodes gRNA for 3' target of human CEBPG |
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| 86346 | PX458_KLF9_1 | Encodes gRNA for 3' target of human KLF9 |
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| 86347 | PX458_KLF9_2 | Encodes gRNA for 3' target of human KLF9 |
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| 86348 | PX458_NFIL3_1 | Encodes gRNA for 3' target of human NFIL3 |
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| 86349 | PX458_NFIL3_2 | Encodes gRNA for 3' target of human NFIL3 |
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| 86350 | PX458_ARID5B_1 | Encodes gRNA for 3' target of human ARID5B |
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| 86351 | PX458_ARID5B_2 | Encodes gRNA for 3' target of human ARID5B |
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| 86352 | PX458_HOMEZ_1 | Encodes gRNA for 3' target of human HOMEZ |
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| 86353 | PX458_HOMEZ_2 | Encodes gRNA for 3' target of human HOMEZ |
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| 86354 | PX458_DMAP1_1 | Encodes gRNA for 3' target of human DMAP1 |
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| 86355 | PX458_DMAP1_2 | Encodes gRNA for 3' target of human DMAP1 |
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| 86356 | PX458_TEAD1_1 | Encodes gRNA for 3' target of human TEAD1 |
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| 86357 | PX458_SOX5_1 | Encodes gRNA for 3' target of human SOX5 |
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| 86358 | PX458_CEBPA_1 | Encodes gRNA for 3' target of human CEBPA |
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| 86359 | PX458_CEBPA_2 | Encodes gRNA for 3' target of human CEBPA |
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| 86360 | PX458_ZFP1_iso1_1 | Encodes gRNA for 3' target of human ZFP1_iso1 |
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| 86361 | PX458_ZFP1_iso1_2 | Encodes gRNA for 3' target of human ZFP1_iso1 |
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| 86362 | PX458_SP5_1 | Encodes gRNA for 3' target of human SP5 |
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| 86363 | PX458_SP5_2 | Encodes gRNA for 3' target of human SP5 |
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| 86364 | PX458_RFXANK_1 | Encodes gRNA for 3' target of human RFXANK |
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| 86365 | PX458_RFXANK_2 | Encodes gRNA for 3' target of human RFXANK |
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| 101069 | pFETCh_RERE | Donor vector for 3' FLAG tag of human RERE |
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| 101070 | pFETCh_KMT2B | Donor vector for 3' FLAG tag of human KMT2B |
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| 101071 | pFETCh_AHR | Donor vector for 3' FLAG tag of human AHR |
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| 101072 | pFETCh_ARID4B | Donor vector for 3' FLAG tag of human ARID4B |
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| 101073 | PX458_RERE_1 | Encodes gRNA for 3' target of human RERE |
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| 101074 | PX458_KMT2B_1 | Encodes gRNA for 3' target of human KMT2B |
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| 101075 | PX458_KMT2B_2 | Encodes gRNA for 3' target of human KMT2B |
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| 101076 | PX458_AHR_1 | Encodes gRNA for 3' target of human AHR |
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| 101077 | PX458_AHR_2 | Encodes gRNA for 3' target of human AHR |
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| 101078 | PX458_ARID4B_1 | Encodes gRNA for 3' target of human ARID4B |
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| 104035 | pFETCh_GABPB1_iso2 | Donor vector for 3' FLAG tag of human GABPB1_iso2 |
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| 104036 | pFETCh_GATAD1 | Donor vector for 3' FLAG tag of human GATAD1 |
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| 104037 | pFETCh_ZNF511 | Donor vector for 3' FLAG tag of human ZNF511 |
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| 104038 | pFETCh_BCL6_iso1 | Donor vector for 3' FLAG tag of human BCL6_iso1 |
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| 104039 | pFETCh_NFIA_iso1 | Donor vector for 3' FLAG tag of human NFIA_iso1 |
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| 104040 | pFETCh_RXRB | Donor vector for 3' FLAG tag of human RXRB |
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| 104041 | PX458_GABPB1_iso2_1 | Encodes gRNA for 3' target of human GABPB1_iso2 along with Cas9 with 2A GFP |
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| 104042 | PX458_GATAD1_1 | Encodes gRNA for 3' target of human GATAD1 along with Cas9 with 2A GFP |
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| 104043 | PX458_GATAD1_2 | Encodes gRNA for 3' target of human GATAD1 along with Cas9 with 2A GFP |
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| 104044 | PX458_ZNF511_1 | Encodes gRNA for 3' target of human ZNF511 along with Cas9 with 2A GFP |
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| 104045 | PX458_ZNF511_2 | Encodes gRNA for 3' target of human ZNF511 along with Cas9 with 2A GFP |
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| 104046 | PX458_BCL6_iso1_1 | Encodes gRNA for 3' target of human BCL6_iso1 along with Cas9 with 2A GFP |
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| 104047 | PX458_BCL6_iso1_2 | Encodes gRNA for 3' target of human BCL6_iso1 along with Cas9 with 2A GFP |
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| 104048 | PX458_NFIA_iso1_1 | Encodes gRNA for 3' target of human NFIA_iso1 along with Cas9 with 2A GFP |
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| 104049 | PX458_NFIA_iso1_2 | Encodes gRNA for 3' target of human NFIA_iso1 along with Cas9 with 2A GFP |
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| 104050 | PX458_RXRB_1 | Encodes gRNA for 3' target of human RXRB along with Cas9 with 2A GFP |
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| 104051 | PX458_RXRB_2 | Encodes gRNA for 3' target of human RXRB along with Cas9 with 2A GFP |
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| 128235 | pFETCh_ZBTB1 | Donor vector for 3' FLAG tag of human ZBTB1 |
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| 128236 | pFETCh_ZNF326_iso1 | Donor vector for 3' FLAG tag of human ZNF326_iso1 |
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| 128238 | pFETCh_SIX4_iso1 | Donor vector for 3' FLAG tag of human SIX4_iso1 |
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| 128239 | pFETCh_ZHX3_iso1 | Donor vector for 3' FLAG tag of human ZHX3_iso1 |
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| 128240 | pFETCh_ZBTB49_iso1 | Donor vector for 3' FLAG tag of human ZBTB49_iso1 |
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| 128241 | pFETCh_SSX2B | Donor vector for 3' FLAG tag of human SSX2B |
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| 128242 | PX458_ZBTB1_1 | Encodes gRNA for 3' target of human ZBTB1 |
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| 128243 | PX458_ZNF326_iso1_1 | Encodes gRNA for 3' target of human ZNF326_iso1 |
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| 128244 | PX458_ZNF326_iso1_2 | Encodes gRNA for 3' target of human ZNF326_iso1 |
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| 128245 | PX458_THRA_iso1_1 | Encodes gRNA for 3' target of human THRA_iso1 |
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| 128246 | PX458_THRA_iso1_2 | Encodes gRNA for 3' target of human THRA_iso1 |
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| 128247 | PX458_SIX4_iso1_1 | Encodes gRNA for 3' target of human SIX4_iso1 |
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| 128248 | PX458_SIX4_iso1_2 | Encodes gRNA for 3' target of human SIX4_iso1 |
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| 128249 | PX458_ZHX3_iso1_1 | Encodes gRNA for 3' target of human ZHX3_iso1 |
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| 128250 | PX458_ZBTB49_iso1_1 | Encodes gRNA for 3' target of human ZBTB49_iso1 |
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| 128251 | PX458_ZBTB49_iso1_2 | Encodes gRNA for 3' target of human ZBTB49_iso1 |
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| 128252 | PX330_SSX2B | Encodes gRNA for 3' target of human SSX2B |
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| 135726 | pFETCh_ZNF331 | Donor vector for 3' FLAG tag of human ZNF331 |
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| 135727 | pFETCh_ZBTB21 | Donor vector for 3' FLAG tag of human ZBTB21 |
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| 135728 | pFETCh_CIC_iso1 | Donor vector for 3' FLAG tag of human CIC_iso1 |
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| 135729 | pFETCh_MLLT10_iso1 | Donor vector for 3' FLAG tag of human MLLT10_iso1 |
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| 135730 | pFETCh_ZBTB7B | Donor vector for 3' FLAG tag of human ZBTB7B |
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| 135731 | pFETCh_ZNF264_iso1 | Donor vector for 3' FLAG tag of human ZNF264_iso1 |
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| 135732 | pFETCh_MAF1_iso1 | Donor vector for 3' FLAG tag of human MAF1_iso1 |
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| 135733 | pFETCh_MED13_iso1 | Donor vector for 3' FLAG tag of human MED13_iso1 |
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| 135734 | pFETCh_FOXM1_iso1 | Donor vector for 3' FLAG tag of human FOXM1_iso1 |
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| 135735 | pFETCh_ZNF503 | Donor vector for 3' FLAG tag of human ZNF503 |
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| 135737 | pFETCh_ELK1_iso1 | Donor vector for 3' FLAG tag of human ELK1_iso1 |
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| 135738 | pFETCh_ONECUT2 | Donor vector for 3' FLAG tag of human ONECUT2 |
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| 135739 | PX458_ZNF331_1 | Encodes gRNA for 3' target of human ZNF331 |
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| 135740 | PX458_ZNF331_2 | Encodes gRNA for 3' target of human ZNF331 |
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| 135741 | PX458_ZBTB21_1 | Encodes gRNA for 3' target of human ZBTB21 |
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| 135742 | PX458_ZBTB21_2 | Encodes gRNA for 3' target of human ZBTB21 |
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| 135743 | PX458_CIC_iso1_1 | Encodes gRNA for 3' target of human CIC_iso1 |
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| 135744 | PX458_CIC_iso1_2 | Encodes gRNA for 3' target of human CIC_iso1 |
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| 135745 | PX330_MLLT10_iso1_1 | Encodes gRNA for 3' target of human MLLT10_iso1 |
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| 135746 | PX330_ZBTB7B_1 | Encodes gRNA for 3' target of human ZBTB7B |
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| 135747 | PX330_ZBTB7B_2 | Encodes gRNA for 3' target of human ZBTB7B |
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| 135748 | PX330_ZNF264_iso1_1 | Encodes gRNA for 3' target of human ZNF264_iso1 |
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| 135749 | PX330_MAF1_iso1_1 | Encodes gRNA for 3' target of human MAF1_iso1 |
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| 135750 | PX330_MAF1_iso1_2 | Encodes gRNA for 3' target of human MAF1_iso1 |
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| 135751 | PX330_MED13_iso1_1 | Encodes gRNA for 3' target of human MED13_iso1 |
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| 135752 | PX458_FOXM1_iso1_1 | Encodes gRNA for 3' target of human FOXM1_iso1 |
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| 135753 | PX458_FOXM1_iso1_2 | Encodes gRNA for 3' target of human FOXM1_iso1 |
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| 135754 | PX330_ZNF503_1 | Encodes gRNA for 3' target of human ZNF503 |
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| 135755 | PX330_ZNF503_2 | Encodes gRNA for 3' target of human ZNF503 |
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| 135756 | PX330_E2F4_iso1_1 | Encodes gRNA for 3' target of human E2F4_iso1 |
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| 135757 | PX330_ELK1_iso1_1 | Encodes gRNA for 3' target of human ELK1_iso1 |
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| 135758 | PX330_ELK1_iso1_2 | Encodes gRNA for 3' target of human ELK1_iso1 |
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| 135759 | PX330_ONECUT2_1 | Encodes gRNA for 3' target of human ONECUT2 |
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| 135760 | PX330_ONECUT2_2 | Encodes gRNA for 3' target of human ONECUT2 |
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