PurposeHomology Arms and 3X Flag with P2A Neo for 3' tagging of human RAD21
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||64056||Standard format: Plasmid sent in bacteria as agar stab||1||$85|
This material is available to academics and nonprofits only.
- Backbone size w/o insert (bp) 5771
- Total vector size (bp) 7200
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Kanamycin, 50 μg/mL
Copy numberHigh Copy
Gene/Insert nameRAD21 Homology Arms
SpeciesH. sapiens (human)
Insert Size (bp)1600
Entrez GeneRAD21 (a.k.a. CDLS4, HR21, HRAD21, MCD1, MGS, NXP1, SCC1, hHR21)
/ Fusion Protein
- 3X Flag P2A Neo (C terminal on insert)
- Cloning method Gibson Cloning
- 5′ sequencing primer acgcctgtgaaaccgtacta
- 3′ sequencing primer aactgttgggaagggcgatc (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
This plasmid has been used along with PX548_RAD21_1 to add a FLAG tag to human RAD21 in HepG2 and MCF7 cells by the Mendenhall and Myers labs at HudsonAlpha Institute for Biotechnology/UAH. Note: This plasmid is part of the Mendenhall and Meyers CRISPR-based Tagging system to add a tag (currently using FLAG) to endogenous proteins. Please see https://www.addgene.org/crispr/tagging/ for more details.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pFETCh_RAD21 was a gift from Eric Mendenhall & Richard M. Myers (Addgene plasmid # 64056 ; http://n2t.net/addgene:64056 ; RRID:Addgene_64056)
For your References section:CETCh-seq: CRISPR epitope tagging ChIP-seq of DNA-binding proteins. Savic D, Partridge EC, Newberry KM, Smith SB, Meadows SK, Roberts BS, Mackiewicz M, Mendenhall EM, Myers RM. Genome Res. 2015 Sep 9. 10.1101/gr.193540.115 PubMed 26355004