Skip to main content
This website uses cookies to ensure you get the best experience. By continuing to use this site, you agree to the use of cookies.

Please note: Your browser does not support the features used on Addgene's website. You may not be able to create an account or request plasmids through this website until you upgrade your browser. Learn more

Please note: Your browser does not fully support some of the features used on Addgene's website. If you run into any problems registering, depositing, or ordering please contact us at [email protected]. Learn more

Addgene

pdCas9-humanized Citations (16)

Originally described in: Repurposing CRISPR as an RNA-Guided Platform for Sequence-Specific Control of Gene Expression.
Qi LS, Larson MH, Gilbert LA, Doudna JA, Weissman JS, Arkin AP, Lim WA Cell. 2013 Feb 28;152(5):1173-83. doi: 10.1016/j.cell.2013.02.022. PubMed Journal

Articles Citing pdCas9-humanized

Articles
CRISPR interference (CRISPRi) for sequence-specific control of gene expression. Larson MH, Gilbert LA, Wang X, Lim WA, Weissman JS, Qi LS. Nat Protoc. 2013 Nov;8(11):2180-96. doi: 10.1038/nprot.2013.132. Epub 2013 Oct 17. PubMed
Sequence-specific inhibition of microRNA via CRISPR/CRISPRi system. Zhao Y, Dai Z, Liang Y, Yin M, Ma K, He M, Ouyang H, Teng CB. Sci Rep. 2014 Feb 3;4:3943. doi: 10.1038/srep03943. PubMed
A CRISPR-based approach for proteomic analysis of a single genomic locus. Waldrip ZJ, Byrum SD, Storey AJ, Gao J, Byrd AK, Mackintosh SG, Wahls WP, Taverna SD, Raney KD, Tackett AJ. Epigenetics. 2014 Sep;9(9):1207-11. doi: 10.4161/epi.29919. Epub 2014 Jul 18. PubMed
Comparison of TALE designer transcription factors and the CRISPR/dCas9 in regulation of gene expression by targeting enhancers. Gao X, Tsang JC, Gaba F, Wu D, Lu L, Liu P. Nucleic Acids Res. 2014 Nov 10;42(20):e155. doi: 10.1093/nar/gku836. Epub 2014 Sep 15. PubMed
Evaluation of sgRNA target sites for CRISPR-mediated repression of TP53. Lawhorn IE, Ferreira JP, Wang CL. PLoS One. 2014 Nov 14;9(11):e113232. doi: 10.1371/journal.pone.0113232. eCollection 2014. PubMed
RNA-guided transcriptional regulation in planta via synthetic dCas9-based transcription factors. Piatek A, Ali Z, Baazim H, Li L, Abulfaraj A, Al-Shareef S, Aouida M, Mahfouz MM. Plant Biotechnol J. 2015 May;13(4):578-89. doi: 10.1111/pbi.12284. Epub 2014 Nov 14. PubMed
Efficient fdCas9 Synthetic Endonuclease with Improved Specificity for Precise Genome Engineering. Aouida M, Eid A, Ali Z, Cradick T, Lee C, Deshmukh H, Atef A, AbuSamra D, Gadhoum SZ, Merzaban J, Bao G, Mahfouz M. PLoS One. 2015 Jul 30;10(7):e0133373. doi: 10.1371/journal.pone.0133373. eCollection 2015. PubMed
Engineering Synthetic Gene Circuits in Living Cells with CRISPR Technology. Jusiak B, Cleto S, Perez-Pinera P, Lu TK. Trends Biotechnol. 2016 Jan 22. pii: S0167-7799(15)00274-7. doi: 10.1016/j.tibtech.2015.12.014. PubMed
Editing DNA Methylation in the Mammalian Genome. Liu XS, Wu H, Ji X, Stelzer Y, Wu X, Czauderna S, Shu J, Dadon D, Young RA, Jaenisch R. Cell. 2016 Sep 22;167(1):233-247.e17. doi: 10.1016/j.cell.2016.08.056. PubMed

Associated Plasmids
Targeted AID-mediated mutagenesis (TAM) enables efficient genomic diversification in mammalian cells. Ma Y, Zhang J, Yin W, Zhang Z, Song Y, Chang X. Nat Methods. 2016 Dec;13(12):1029-1035. doi: 10.1038/nmeth.4027. Epub 2016 Oct 10. PubMed

Associated Plasmids
Notch regulates BMP responsiveness and lateral branching in vessel networks via SMAD6. Mouillesseaux KP, Wiley DS, Saunders LM, Wylie LA, Kushner EJ, Chong DC, Citrin KM, Barber AT, Park Y, Kim JD, Samsa LA, Kim J, Liu J, Jin SW, Bautch VL. Nat Commun. 2016 Nov 11;7:13247. doi: 10.1038/ncomms13247. PubMed
Targeted DNA methylation in pericentromeres with genome editing-based artificial DNA methyltransferase. Yamazaki T, Hatano Y, Handa T, Kato S, Hoida K, Yamamura R, Fukuyama T, Uematsu T, Kobayashi N, Kimura H, Yamagata K. PLoS One. 2017 May 18;12(5):e0177764. doi: 10.1371/journal.pone.0177764. eCollection 2017. PubMed
Comparative analysis of chimeric ZFP-, TALE- and Cas9-piggyBac transposases for integration into a single locus in human cells. Luo W, Galvan DL, Woodard LE, Dorset D, Levy S, Wilson MH. Nucleic Acids Res. 2017 Jun 29. doi: 10.1093/nar/gkx572. PubMed
Inhibition of O-GlcNAcase Sensitizes Apoptosis and Reverses Bortezomib Resistance in Mantle Cell Lymphoma through Modification of Truncated Bid. Luanpitpong S, Chanthra N, Janan M, Poohadsuan J, Samart P, U-Pratya Y, Rojanasakul Y, Issaragrisil S. Mol Cancer Ther. 2018 Feb;17(2):484-496. doi: 10.1158/1535-7163.MCT-17-0390. Epub 2017 Nov 22. PubMed
Chemical Inducible dCas9-Guided Editing of H3K27 Acetylation in Mammalian Cells. Gao D, Liang FS. Methods Mol Biol. 2018;1767:429-445. doi: 10.1007/978-1-4939-7774-1_24. PubMed
The fundamental role of chromatin loop extrusion in physiological V(D)J recombination. Zhang Y, Zhang X, Ba Z, Liang Z, Dring EW, Hu H, Lou J, Kyritsis N, Zurita J, Shamim MS, Presser Aiden A, Lieberman Aiden E, Alt FW. Nature. 2019 Sep;573(7775):600-604. doi: 10.1038/s41586-019-1547-y. Epub 2019 Sep 11. PubMed

If you have published an article using this material, please email us at [email protected] to have your article added to this page.