PurposeExpresses fusion of human codon-optimized S. pyogenes dCas9 (D10A, H840A ) protein with a human AID mutant protein (p182X). MSCV Retroviral backbone.
Full plasmid sequence is not available for this item.
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||83260||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Vector typeMammalian Expression, Retroviral
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Gene/Insert namedCas9-AID P182X
Insert Size (bp)5000
MutationD10A and H840A in Cas9; P182X in AID
- Promoter MSCV
/ Fusion Proteins
- 3xFLAG-NLS (N terminal on insert)
- FLAG (C terminal on insert)
- Cloning method Restriction Enzyme
- 5′ cloning site BglII (not destroyed)
- 3′ cloning site XhoI (not destroyed)
- 5′ sequencing primer pLXSN-F (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:MO91-dCas9-AID(P182X) was a gift from Xing Chang (Addgene plasmid # 83260 ; http://n2t.net/addgene:83260 ; RRID:Addgene_83260)
For your References section:Targeted AID-mediated mutagenesis (TAM) enables efficient genomic diversification in mammalian cells. Ma Y, Zhang J, Yin W, Zhang Z, Song Y, Chang X. Nat Methods. 2016 Dec;13(12):1029-1035. doi: 10.1038/nmeth.4027. Epub 2016 Oct 10. 10.1038/nmeth.4027 PubMed 27723754