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MO91-dCas9-AID(P182X)
(Plasmid #83260)

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Full plasmid sequence is not available for this item.

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Item Catalog # Description Quantity Price (USD)
Plasmid 83260 Standard format: Plasmid sent in bacteria as agar stab 1 $65

This material is available to academics and nonprofits only.

Backbone

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin
  • Growth Temperature
    37°C
  • Growth Strain(s)
    Stbl3
  • Copy number
    Unknown

Gene/Insert

  • Gene/Insert name
    dCas9-AID P182X
  • Species
    Synthetic
  • Insert Size (bp)
    5000
  • Mutation
    D10A and H840A in Cas9; P182X in AID
  • Promoter MSCV
  • Tags / Fusion Proteins
    • 3xFLAG-NLS (N terminal on insert)
    • FLAG (C terminal on insert)

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ cloning site BglII (not destroyed)
  • 3′ cloning site XhoI (not destroyed)
  • 5′ sequencing primer pLXSN-F
  • (Common Sequencing Primers)

Resource Information

  • Terms and Licenses
How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    MO91-dCas9-AID(P182X) was a gift from Xing Chang (Addgene plasmid # 83260 ; http://n2t.net/addgene:83260 ; RRID:Addgene_83260)
  • For your References section:

    Targeted AID-mediated mutagenesis (TAM) enables efficient genomic diversification in mammalian cells. Ma Y, Zhang J, Yin W, Zhang Z, Song Y, Chang X. Nat Methods. 2016 Dec;13(12):1029-1035. doi: 10.1038/nmeth.4027. Epub 2016 Oct 10. 10.1038/nmeth.4027 PubMed 27723754