163,347 results

1. p2T-cmv-SpCas9-TadCBEd-BlastR

   Plasmid

   #193848

   Purpose

   Express TadCBEd in mammalian cells with BlastR selection, Tol2 integration

   Depositor

   David Liu

   Article

   Neugebauer et al Nat Biotechnol. 2022 Nov 10. pii: 10.103

   Insert

   TadCBEd
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   Dec. 15, 2022

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

2. pEYFP-C3-HA-Ubiquitin

   Plasmid

   #28236

   Depositor

   Michael Mancini

   Article

   Stenoien et al Nat Cell Biol. 2002 Oct . 4(10):806-10.

   Insert

   Ubiquitin (UBB Human)
   

   Use

   Tags

   EYFP and HA

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   April 7, 2011

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. CP1041-ABE8e

   Plasmid

   #138493

   Purpose

   A-to-G base editor

   Depositor

   David Liu

   Article

   Richter et al Nat Biotechnol. 2020 Jul;38(7):883-891.

   Insert

   ecTadA(8e)-nSpCas9 (CP1041)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   March 16, 2020

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. OptoEphB2-mCherry

   Plasmid

   #92042

   Purpose

   Expresses OptoEphB2 labeled with mCherry

   Depositor

   Ji Yu

   Insert

   EPHB2 (EPHB2, CRY2 Human, Mustard Weed)
   

   Use

   Tags

   mCherry

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 10, 2017

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

5. pCXLE-hOCT3/4-shp53-F + mCherry-2A-puro

   Plasmid

   #74947

   Purpose

   Dosage control and tracing of reprogramming episomes

   Depositor

   Ann Zovein

   Article

   Schmitt et al Stem Cell Res Ther. 2017 Jun 5;8(1):132.

   Insert

   Oct3/4 (POU5F1 Human)
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   May 12, 2017

   Availability

   Academic Institutions and Nonprofits only

   

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6. pQCXIP-SHMY-PAUF

   Plasmid

   #182645

   Purpose

   Mammalian expression vector encoding a protein fusion composed of an N-terminal ER targeting sequence of hemagglutinin (HA), one tyrosine sulfation (TS) motif (Y), myc (M), His6 (H) and PAUF sequence

   Depositor

   Martin Spiess

   Article

   Buser et al Life Sci Alliance. 2022 Mar 21;5(7). pii

   Insert

   Pancreatic adenocarcinoma upregulated factor (PAUF)
   

   Use

   Retroviral

   Tags

   His6-tag, N-terminal ER targeting sequence of hem…

   Expression

   Mammalian

   Mutation

   Promoter

   CMV

   Available since

   April 22, 2022

   Availability

   Academic Institutions and Nonprofits only

   

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7. pIII/MS2-1

   Plasmid

   #220629

   Purpose

   Yeast/E. coli shuttle vector in which an RNA polymerase III promoter directs transcription of an RNA containing two tandem MS2 sites.

   Depositor

   Marvin Wickens

   Article

   Bernstein et al Methods. 2002 Feb;26(2):123-41. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. pIII/MS2-2

   Plasmid

   #220630

   Purpose

   For expressing an RNA containing your sequence of interest adjacent to two MS2 sites from an RNA polymerase III promoter.

   Depositor

   Marvin Wickens

   Article

   Bernstein et al Methods. 2002 Feb;26(2):123-41. doi: 10.

   Type

   Empty backbone

   Use

   Tags

   Expression

   Yeast

   Mutation

   Promoter

   Available since

   Oct. 9, 2024

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. BCL-XL-pcw107-V5

   Plasmid

   #64631

   Purpose

   when used to produce lentivirus, express physiological levels of insert

   Depositor

   David Sabatini, Kris Wood

   Article

   Martz et al Sci Signal. 2014 Dec 23;7(357):ra121. do

   Insert

   BCL2L1 (transcript variant 1) (BCL2L1 Human)
   

   Use

   Lentiviral

   Tags

   V5

   Expression

   Mutation

   Promoter

   PGK

   Available since

   May 12, 2015

   Availability

   Academic Institutions and Nonprofits only

10. pENTR-p38KTRClover

    Plasmid

    #59142

    Purpose

    ORF encoding p38 KTR mClover flanked by Gateway sequences

    Depositor

    Markus Covert

    Article

    Regot et al Cell. 2014 Jun 19;157(7):1724-34. doi: 1

    Insert

    p38 Kinase Translocation Reporter (MAPK14 Human, Mouse)
    

    Use

    Tags

    Expression

    Mutation

    Promoter

    Available since

    Sept. 3, 2014

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. pY027

    Plasmid

    #84742

    Purpose

    Expresses huLbCpf1 and crRNA guide

    Depositor

    Feng Zhang

    Article

    Zetsche et al Nat Biotechnol. 2017 Jan;35(1):31-34. do

    Insert

    huLbCpf1
    

    Use

    CRISPR

    Tags

    3xHA and NLS

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 4, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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12. pCMV-MMLV-gag-hMLH1dn

    Plasmid

    #195513

    Purpose

    MMLV-Gag fused to hMLH1dn with TSTLLMENSS cleavage site between Gag-NES and hMLH1dn. For production of virus like particles with base-editor RNP cargo.

    Depositor

    Hyongbum Kim

    Article

    Yu et al Cell. 2023 Apr 21:S0092-8674(23)00331-8.

    Insert

    hMLH1dn (MLH1 Human)
    

    Use

    Virus like particle

    Tags

    FLAG, NES, and Protease cleavate site

    Expression

    Mammalian

    Mutation

    Human MLH1 del754-756

    Promoter

    CMV

    Available since

    May 2, 2023

    Availability

    Academic Institutions and Nonprofits only

    

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13. pAraH6HATT

    Plasmid

    #63901

    Purpose

    For expression of soluble scFv/Fab fragments in bacteria from genes selected using the pComb3H system, contains TT Fab (light and heavy chains) against tetanus toxin, used as expression control.

    Depositor

    Carlos Barbas

    Article

    Barbas et al Methods, A companion to Methods in Enzym

    Insert

    TT Fab
    

    Use

    Tags

    6xHis and HA tag

    Expression

    Bacterial

    Mutation

    Promoter

    araBAD

    Available since

    Feb. 2, 2016

    Availability

    Academic Institutions and Nonprofits only

    

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14. pNup133-Ypet

    Plasmid

    #98283

    Purpose

    mammalian expression of Nup133-Ypet

    Depositor

    Mike Heilemann

    Article

    Finan et al Angew Chem Int Ed Engl. 2015 Oct 5;54(41

    Insert

    Nup133 (NUP133 Human)
    

    Use

    Tags

    YPet

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    Nov. 21, 2017

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. pRS426 Gal TDP43

    Plasmid

    #27466

    Depositor

    Aaron Gitler

    Article

    Johnson et al Proc Natl Acad Sci U S A. 2008 Apr 29. 1

    Insert

    TDP-43 (TARDBP Human)
    

    Use

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    Jan. 31, 2011

    Availability

    Academic Institutions and Nonprofits only

16. pScalps_Puro_mTet2 catalytic domain

    Plasmid

    #79554

    Purpose

    Expression of catalytic domain of mouse Tet2

    Depositor

    Silvia Monticelli

    Article

    Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

    Insert

    Tet2 (Tet2 Mouse)
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mutation

    aa990-1912

    Promoter

    Available since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only

17. RET-V5

    Plasmid

    #139627

    Purpose

    V5-His-tagged gene expression

    Depositor

    Markku Varjosalo

    Article

    Yadav et al iScience. 2020 Feb 21;23(2):100871. doi:

    Insert

    RET (RET Human)
    

    Use

    Tags

    3xV5-6xHis

    Expression

    Mammalian

    Mutation

    Promoter

    CMV

    Available since

    May 13, 2020

    Availability

    Academic Institutions and Nonprofits only

    

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18. SL-SunTag

    Plasmid

    #196919

    Purpose

    The SunTag-Renilla-MS2 reporter containing cap-proximal GC-rich stem-loop

    Depositor

    Jeffrey Chao

    Article

    Dave et al Mol Cell. 2023 Feb 16;83(4):589-606.e6.

    Insert

    Renilla luciferase
    

    Use

    Tags

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    March 15, 2023

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pCMV-Cas9-NRTH

    Plasmid

    #136925

    Purpose

    Expresses Cas9-NRTH in mammalian cells

    Depositor

    David Liu

    Article

    Miller et al Nat Biotechnol. 2020 Apr;38(4):471-481.

    Insert

    Cas9-NRTH
    

    Use

    CRISPR

    Tags

    Expression

    Mammalian

    Mutation

    see manuscript

    Promoter

    CMV

    Available since

    Feb. 11, 2020

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. pScalps_Puro_mTet2 catalytic domain HxD

    Plasmid

    #79611

    Purpose

    Expression of catalytically inactive mouse Tet2

    Depositor

    Silvia Monticelli

    Article

    Montagner et al Cell Rep. 2016 May 17;15(7):1566-79. doi

    Insert

    Tet2 (Tet2 Mouse)
    

    Use

    Lentiviral

    Tags

    Myc

    Expression

    Mutation

    Mutant Tet2 with H1302Y, D1304A substitutions in …

    Promoter

    Available since

    July 21, 2016

    Availability

    Academic Institutions and Nonprofits only