163,387 results

1. pAAV2/6 v1

   Plasmid

   #240485

   Purpose

   AAV packaging plasmid expressing AAV2 Rep protein and AAV6 capsid protein

   Depositor

   Meghan Rego, Addgene Research Program

   Insert

   AAV2 Rep and AAV6 Cap
   

   Use

   AAV

   Tags

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 25, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

2. pHS_Nova

   Plasmid

   #239831

   Purpose

   NovaIscB mammalian expression

   Depositor

   Feng Zhang

   Article

   Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

   Insert

   NovaIscB
   

   Use

   Tags

   Nucleoplasmin NLS-3xHA and SV40 NLS

   Expression

   Mammalian

   Mutation

   Insertion of REC domain from NbaCas9-1 and swap o…

   Promoter

   CMV

   Available since

   July 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

3. pHS2002

   Plasmid

   #239832

   Purpose

   Engineered OrufIscB (NovaIscB) omegaRNA mammalian expression

   Depositor

   Feng Zhang

   Article

   Kannan et al Nat Biotechnol. 2025 May 7. doi: 10.1038

   Insert

   NovaIscB omegaRNA scaffold
   

   Use

   Tags

   Expression

   Mammalian

   Mutation

   Replacement of A12-A57 with GAAA in omegaRNA scaf…

   Promoter

   human U6

   Available since

   July 21, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

4. CFTR-HiBiT Fusion Vector

   Plasmid

   #236890

   Purpose

   Express CFTR WT HiBiT in Mammalian Cells under a CMV promoter

   Depositor

   Promega Early Access Program

   Has Service

   DNA

   Insert

   CFTR (CFTR Human)
   

   Use

   Tags

   HiBiT

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CMV

   Available since

   July 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

5. NanoBRET BiBRET Vector, NanoLuc-HRAS WT-CRAF RBD-HaloTag

   Plasmid

   #236861

   Purpose

   Express NanoLuc(R)-HRAS WT-CRAF RBD-HaloTag(R) in Mammalian Cells under a CMV promoter

   Depositor

   Promega Early Access Program

   Has Service

   DNA

   Inserts

   HRAS (HRAS Human)
   cRAF(RBD) (RAF1 Human)
   

   Use

   Tags

   HaloTag (R) and NanoLuc (R)

   Expression

   Mammalian

   Mutation

   None

   Promoter

   CMV

   Available since

   July 14, 2025

   Availability

   Industry, Academic Institutions, and Nonprofits

   

   Enlarge

6. Tad-CBE(TCG2)

   Plasmid

   #235068

   Purpose

   Expresses Tad-CBE(TCG2) in mammalian cells.

   Depositor

   Weixin Tang

   Article

   Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

   Insert

   Tad-CBE(TCG2)-SpCas9 D10A
   

   Use

   CRISPR

   Tags

   2xUGI, bpNLS and bpNLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

7. Tad-CBE(CCA3)

   Plasmid

   #235070

   Purpose

   Expresses Tad-CBE(CCA3) in mammalian cells.

   Depositor

   Weixin Tang

   Article

   Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

   Insert

   Tad-CBE(CCA3)-SpCas9 D10A
   

   Use

   CRISPR

   Tags

   2xUGI, bpNLS and bpNLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

8. Tad-CBE(CCT2)

   Plasmid

   #235071

   Purpose

   Expresses Tad-CBE(CCT2) in mammalian cells.

   Depositor

   Weixin Tang

   Article

   Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

   Insert

   Tad-CBE(CCT2)-SpCas9 D10A
   

   Use

   CRISPR

   Tags

   2xUGI, bpNLS and bpNLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

9. Tad-CBE(TCC2)

   Plasmid

   #235067

   Purpose

   Expresses Tad-CBE(TCC2) in mammalian cells.

   Depositor

   Weixin Tang

   Article

   Wu et al Nat Biotechnol. 2025 Jul 7:10.1038/s4158

   Insert

   Tad-CBE(TCC2)-SpCas9 D10A
   

   Use

   CRISPR

   Tags

   2xUGI, bpNLS and bpNLS

   Expression

   Mammalian

   Mutation

   Promoter

   Available since

   July 14, 2025

   Availability

   Academic Institutions and Nonprofits only

   

   Enlarge

10. pMTKM05

    Plasmid

    #233477

    Purpose

    SpCAS9-Tyr-[gRNA: BsaI GFP dropout] with pan(OPT)ARS replication origin and Hyg selection.

    Depositor

    Vincent Martin

    Article

    Thornbury et al ACS Synth Biol. 2025 Jul 8. doi: 10.1021

    Type

    Empty backbone

    Use

    CRISPR and Synthetic Biology

    Tags

    Expression

    Yeast

    Mutation

    Promoter

    Available since

    July 2, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

11. AAV-DIO-Bmal1

    Plasmid

    #239045

    Purpose

    Overexpression of Bmal1

    Depositor

    Qingchun Tong

    Article

    Kim et al Nat Commun. 2020 Jul 30;11(1):3794. doi:

    Insert

    mArntl (Bmal1 Mouse)
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    Bmal1

    Available since

    May 21, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

12. AiP13899 - pAAV-AiE0494m_3xC2-minBG-SYFP2-WPRE3-BGHpA (Alias: CN3899)

    Plasmid

    #230385

    Purpose

    AiE0494m_3xC2 is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

    Depositor

    The Allen Institute for Brain Science, Jonathan Ting

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minBG

    Available since

    April 28, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

13. AiP1365 - pAAV-AiE2136m-minBG-SYFP2-WPRE3-BGHpA

    Plasmid

    #230630

    Purpose

    AiE2136m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

    Depositor

    Bosiljka Tasic, The Allen Institute for Brain Science

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minBG

    Available since

    March 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

14. AiP1376 - pAAV-AiE2147m-minBG-SYFP2-WPRE-BGHpA

    Plasmid

    #220645

    Purpose

    AiE2147m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

    Depositor

    Bosiljka Tasic, The Allen Institute for Brain Science

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minBG

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

15. AiP11576 - pAAV-hsA2-AiE0082m-minRho-SYFP2-WPRE3-BGHpA (Alias: CN1576)

    Plasmid

    #230354

    Purpose

    AiE0082m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

    Depositor

    The Allen Institute for Brain Science, Jonathan Ting

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minRho

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

16. AiP12861 - pAAV-AiE0666m-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2861)

    Plasmid

    #230139

    Purpose

    AiE0666m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

    Depositor

    Boaz Levi, The Allen Institute for Brain Science

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minBG

    Available since

    Feb. 20, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

17. pLentiCMV Puro DEST P38KTRDronpa

    Plasmid

    #205763

    Purpose

    Expression of P38 KTR Dronpa under CMV promoter (With Puromycin Resistance)

    Depositor

    Edward Boyden

    Article

    Qian et al Cell. 2023 Dec 7;186(25):5656-5672.e21.

    Insert

    P38 KTR Dronpa
    

    Use

    Lentiviral

    Tags

    Expression

    Mammalian

    Mutation

    wt

    Promoter

    Available since

    Jan. 24, 2025

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

18. pcDNA3.1/V5-His-mPer1

    Plasmid

    #219421

    Purpose

    Expresses 3873 bp of mPer1 coding sequence in E. coli.

    Depositor

    Aziz Sancar

    Article

    Cao et al Proc Natl Acad Sci U S A. 2023 Oct 24;12

    Insert

    mPer1 (Per1 Mouse)
    

    Use

    Tags

    His and V5

    Expression

    Bacterial and Mammalian

    Mutation

    Promoter

    Available since

    Dec. 13, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

19. pET-28a(+)-T7-OmpA-MNaseA-6xHis

    Plasmid

    #214808

    Purpose

    Expression of 6xhis-tagged micrococcal nuclease A (MNaseA)

    Depositor

    Peter Sarin

    Article

    Gregorova et al J Biol Chem. 2024 Nov 27;301(1):108020.

    Insert

    OmpA-Micrococcal nuclease A
    

    Use

    Tags

    6xhis

    Expression

    Bacterial

    Mutation

    Promoter

    T7 promoter

    Available since

    Nov. 27, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge

20. AiP1962 - pAAV-AiE2591m-minBG-SYFP2-WPRE-BGHpA

    Plasmid

    #214592

    Purpose

    AiE2591m is an enhancer sequence, designed to drive AAV-mediated transgene expression in specific populations of brain cells

    Depositor

    Bosiljka Tasic, The Allen Institute for Brain Science

    Article

    Ben-Simon et al Cell. 2025 May 16:S0092-8674(25)00513-6.

    Insert

    SYFP2
    

    Use

    AAV

    Tags

    Expression

    Mutation

    Promoter

    minBG

    Available since

    June 24, 2024

    Availability

    Academic Institutions and Nonprofits only

    

    Enlarge