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lenti sgRNA(MS2)_puro backbone Citations (16)

Originally described in: Genome-scale transcriptional activation by an engineered CRISPR-Cas9 complex.
Konermann S, Brigham MD, Trevino AE, Joung J, Abudayyeh OO, Barcena C, Hsu PD, Habib N, Gootenberg JS, Nishimasu H, Nureki O, Zhang F Nature. 2014 Dec 10. doi: 10.1038/nature14136.
PubMed Journal

Articles Citing lenti sgRNA(MS2)_puro backbone

Articles
Genome-scale CRISPR-Cas9 knockout and transcriptional activation screening. Joung J, Konermann S, Gootenberg JS, Abudayyeh OO, Platt RJ, Brigham MD, Sanjana NE, Zhang F. Nat Protoc. 2017 Apr;12(4):828-863. doi: 10.1038/nprot.2017.016. Epub 2017 Mar 23. PubMed

Associated Plasmids

Genome-scale activation screen identifies a lncRNA locus regulating a gene neighbourhood. Joung J, Engreitz JM, Konermann S, Abudayyeh OO, Verdine VK, Aguet F, Gootenberg JS, Sanjana NE, Wright JB, Fulco CP, Tseng YY, Yoon CH, Boehm JS, Lander ES, Zhang F. Nature. 2017 Aug 11. doi: 10.1038/nature23451. PubMed
Frequent sgRNA-barcode recombination in single-cell perturbation assays. Xie S, Cooley A, Armendariz D, Zhou P, Hon GC. PLoS One. 2018 Jun 6;13(6):e0198635. doi: 10.1371/journal.pone.0198635. eCollection 2018. PubMed
Dynamic ubiquitylation of Sox2 regulates proteostasis and governs neural progenitor cell differentiation. Cui CP, Zhang Y, Wang C, Yuan F, Li H, Yao Y, Chen Y, Li C, Wei W, Liu CH, He F, Liu Y, Zhang L. Nat Commun. 2018 Nov 7;9(1):4648. doi: 10.1038/s41467-018-07025-z. PubMed
Long noncoding RNA PiHL regulates p53 protein stability through GRWD1/RPL11/MDM2 axis in colorectal cancer. Deng X, Li S, Kong F, Ruan H, Xu X, Zhang X, Wu Z, Zhang L, Xu Y, Yuan H, Peng H, Yang D, Guan M. Theranostics. 2020 Jan 1;10(1):265-280. doi: 10.7150/thno.36045. eCollection 2020. PubMed
Epigenetic Silencing of CDR1as Drives IGF2BP3-Mediated Melanoma Invasion and Metastasis. Hanniford D, Ulloa-Morales A, Karz A, Berzoti-Coelho MG, Moubarak RS, Sanchez-Sendra B, Kloetgen A, Davalos V, Imig J, Wu P, Vasudevaraja V, Argibay D, Lilja K, Tabaglio T, Monteagudo C, Guccione E, Tsirigos A, Osman I, Aifantis I, Hernando E. Cancer Cell. 2020 Jan 13;37(1):55-70.e15. doi: 10.1016/j.ccell.2019.12.007. PubMed
RIPK3 upregulation confers robust proliferation and collateral cystine-dependence on breast cancer recurrence. Lin CC, Mabe NW, Lin YT, Yang WH, Tang X, Hong L, Sun T, Force J, Marks JR, Yao TP, Alvarez JV, Chi JT. Cell Death Differ. 2020 Jul;27(7):2234-2247. doi: 10.1038/s41418-020-0499-y. Epub 2020 Jan 27. PubMed
Prioritizing disease and trait causal variants at the TNFAIP3 locus using functional and genomic features. Ray JP, de Boer CG, Fulco CP, Lareau CA, Kanai M, Ulirsch JC, Tewhey R, Ludwig LS, Reilly SK, Bergman DT, Engreitz JM, Issner R, Finucane HK, Lander ES, Regev A, Hacohen N. Nat Commun. 2020 Mar 6;11(1):1237. doi: 10.1038/s41467-020-15022-4. PubMed
BAP1/ASXL complex modulation regulates epithelial-mesenchymal transition during trophoblast differentiation and invasion. Perez-Garcia V, Lea G, Lopez-Jimenez P, Okkenhaug H, Burton GJ, Moffett A, Turco MY, Hemberger M. Elife. 2021 Jun 25;10. pii: 63254. doi: 10.7554/eLife.63254. PubMed
AT-rich interaction domain 5A regulates the transcription of interleukin-6 gene in prostate cancer cells. Ikeuchi W, Wakita Y, Zhang G, Li C, Itakura K, Yamakawa T. Prostate. 2022 Jan;82(1):97-106. doi: 10.1002/pros.24251. Epub 2021 Oct 11. PubMed
TCOF1 upregulation in triple-negative breast cancer promotes stemness and tumour growth and correlates with poor prognosis. Hu J, Lai Y, Huang H, Ramakrishnan S, Pan Y, Ma VWS, Cheuk W, So GYK, He Q, Geoffrey Lau C, Zhang L, Cho WCS, Chan KM, Wang X, Rebecca Chin Y. Br J Cancer. 2022 Jan;126(1):57-71. doi: 10.1038/s41416-021-01596-3. Epub 2021 Oct 30. PubMed
A CRISPR Activation Screen Identifies an Atypical Rho GTPase That Enhances Zika Viral Entry. Luu AP, Yao Z, Ramachandran S, Azzopardi SA, Miles LA, Schneider WM, Hoffmann HH, Bozzacco L, Garcia G Jr, Gong D, Damoiseaux R, Tang H, Morizono K, Rudin CM, Sun R, Arumugaswami V, Poirier JT, MacDonald MR, Rice CM, Li MMH. Viruses. 2021 Oct 20;13(11). pii: v13112113. doi: 10.3390/v13112113. PubMed
The risk variant rs11836367 contributes to breast cancer onset and metastasis by attenuating Wnt signaling via regulating NTN4 expression. Yang H, Ting X, Geng YH, Xie Y, Nierenberg JL, Huo YF, Zhou YT, Huang Y, Yu YQ, Yu XY, Li XF, Ziv E, Zhang H, Fang WG, Shen Y, Tian XX. Sci Adv. 2022 Jun 10;8(23):eabn3509. doi: 10.1126/sciadv.abn3509. Epub 2022 Jun 10. PubMed
Genome-wide CRISPR/Cas9 transcriptional activation screen identifies a histone acetyltransferase inhibitor complex as a regulator of HIV-1 integration. Zhang Q, Wang S, Li W, Yau E, Hui H, Singh PK, Achuthan V, Young Karris MA, Engelman AN, Rana TM. Nucleic Acids Res. 2022 Jun 17. pii: 6609817. doi: 10.1093/nar/gkac464. PubMed
Comparative analysis of dCas9-VP64 variants and multiplexed guide RNAs mediating CRISPR activation. Omachi K, Miner JH. PLoS One. 2022 Jun 28;17(6):e0270008. doi: 10.1371/journal.pone.0270008. eCollection 2022. PubMed

Associated Plasmids

Genome-wide gain-of-function screening characterized lncRNA regulators for tumor immune response. Wang Y, Zhao Y, Guo W, Yadav GS, Bhaskarla C, Wang Z, Wang X, Li S, Wang Y, Chen Y, Pattarayan D, Xie W, Li S, Lu B, Kammula US, Zhang M, Yang D. Sci Adv. 2022 Dec 9;8(49):eadd0005. doi: 10.1126/sciadv.add0005. Epub 2022 Dec 7. PubMed

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