Comparative analysis of dCas9-VP64 variants and multiplexed guide RNAs mediating CRISPR activation.
Omachi K, Miner JH
PLoS One. 2022 Jun 28;17(6):e0270008. doi: 10.1371/journal.pone.0270008. eCollection 2022.
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Plasmids from Article
| ID | Plasmid | Purpose |
|---|---|---|
| 192650 | Lenti-EF1alpha-VP64-dCas9-VP64_Blast | 3rd generation lenti vector encoding VP64-dCas9-VP64 with 2A Blast resistance marker |
| 192651 | Lenti-EF1alpha-dCas9-10xGCN4_Hygro | 3rd generation lenti vector encoding dCas9-10xGCN4 (Suntag) with 2A Hygro resistance marker |
| 192652 | Lenti-EF1alpha-scFv-p65-HSF1-Blast | 3rd generation lenti vector encoding scFV-p65-HSF1 with 2A Blast resistance marker |
| 192653 | Lenti-EF1alpha-dCas9-p300_Blast | 3rd generation lenti vector encoding dCas9-p300 with 2A Blast resistance marker |
| 192654 | Lenti-EF1alpha-VP64-dCas9-p300_Blast | 3rd generation lenti vector encoding VP64-dCas9-p300 with 2A Blast resistance marker |
| 192655 | Lenti-EF1alpha-scFv-VP64_Blast | 3rd generation lenti vector encoding scFv-VP64 with 2A Blast resistance marker |
| 192656 | pCR-U6-gRNA-miniCMV-TdTomato | A plasmid encoding miniCMV sgRNA and miniCMV-promoter-TdTomato |
| 192657 | pCR-U6-gRNA-miniCMV-Nluc | A plasmid encoding miniCMV sgRNA and miniCMV-promoter-Nluc |
| 192658 | pCR-U6-gRNA-TRE3G-TdTomato | A plasmid encoding TRE3G sgRNA and TRE3G-promoter-TdTomato |
| 192659 | pCR-U6-gRNA-TRE3G-Nluc | A plasmid encoding TRE3G sgRNA and TRE3G-promoter-Nluc |
| 192670 | LentiU6-hASCL1 gRNA_1-MS2-Puro | Lentiviral expression of sgRNA targeting hASCL1 promoter to activate human ASCL1 transcription |
| 192673 | LentiU6-hMYOD1 gRNA_1-MS2-Puro | Lentiviral expression of sgRNA targeting hMYOD1 promoter to activate human MYOD1 transcription |
| 192676 | LentiU6-hNEUROD1 gRNA_1-MS2-Puro | Lentiviral expression of sgRNA targeting hNEUROD1 promoter to activate human NEUROD1 transcription |
| 192677 | LentiU6-hIL1RN gRNA_1-MS2-Puro | Lentiviral expression of sgRNA targeting hIL1RN promoter to activate human IL1RN transcription |
| 192680 | LentiU6-mNeurog2 gRNA_1-MS2-Puro | Lentiviral expression of sgRNA targeting mIL1RN promoter to activate mouse Neurog2 transcription |
| 192681 | LentiU6-mHbb-bh1_ gRNA_1-MS2-Puro | Lentiviral expression of sgRNA targeting mHbb-bh1promoter to activate mouse Hbb-bh1 transcription |
| 192682 | LentiU6-hASCL1 gRNA_multi1-3-MS2-Puro | Lentiviral expression of multi gRNAs targeting hASCL1 promoter to activate human ASCL1 transcription |
| 192683 | LentiU6-hMYOD1 gRNA_multi1-3-MS2-Puro | Lentiviral expression of multi gNAs targeting hMYOD1 promoter to activate human MYOD1 transcription |
| 192684 | LentiU6-hIL2RN gRNA_multi1-3-MS2-Puro | Lentiviral expression of multi gRNAs targeting hIL1RN promoter to activate human IL1RN transcription |