LentiU6-hIL1RN gRNA_1-MS2-Puro
(Plasmid #192677)

• Purpose: Lentiviral expression of sgRNA targeting hIL1RN promoter to activate human IL1RN transcription
• Depositing Lab: Jeffrey Miner
• Publication: Omachi et al PLoS One. 2022 Jun 28;17(6):e0270008. doi: 10.1371/journal.pone.0270008. eCollection 2022.

Backbone

• Vector backbone: pLenti
• Vector type: Mammalian Expression, Lentiviral, CRISPR

Growth in Bacteria

• Bacterial Resistance(s): Ampicillin, 100 μg/mL
• Growth Temperature: 37°C
• Growth Strain(s): NEB Stable
• Copy number: High Copy

Gene/Insert

• Gene/Insert name: Human IL1RN activating gRNA #1
• gRNA/shRNA sequence: GCATCAAGTCAGCCATCAGC
• Species: H. sapiens (human)
• Entrez Gene: IL1RN (a.k.a. CRMO2, DIRA, ICIL-1RA, IL-1RN, IL-1ra, IL-1ra3, IL1F3, IL1RA, IRAP, MVCD4)
• Promoter: U6

Cloning Information

• Cloning method: Restriction Enzyme
• 5′ cloning site: Unknown (unknown if destroyed)
• 3′ cloning site: Unknown (unknown if destroyed)

Terms and Licenses

• Academic/Nonprofit Terms:
  • UBMTA
• Industry Terms:
  • Not Available to Industry