PurposeExpresses GFP from internal ribosome entry sequence, cloning component
|Item||Catalog #||Description||Quantity||Price (USD)|
|Plasmid||51406||Standard format: Plasmid sent in bacteria as agar stab||1||$75|
This material is available to academics and nonprofits only.
Backbone manufacturerLife Technologies
- Backbone size w/o insert (bp) 5440
- Total vector size (bp) 6772
Modifications to backbonenone
Vector typeMammalian Expression
Selectable markersNeomycin (select with G418)
Growth in Bacteria
Bacterial Resistance(s)Ampicillin, 100 μg/mL
Gene/Insert nameIRES GFP
Alt nameinternal ribosome entry site
Alt namegreen fluorescent protein
Insert Size (bp)1332
- Promoter CMV
- Cloning method Restriction Enzyme
- 5′ cloning site XhoI (not destroyed)
- 3′ cloning site XhoI (destroyed during cloning)
- 5′ sequencing primer T7
- 3′ sequencing primer BGH reverse (Common Sequencing Primers)
Terms and Licenses
- Not Available to Industry
- Zeocin® is an InvivoGen trademark.
The discrepancies between the Addgene QC sequence and the full sequence have no functional consequence.
These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
For your Materials & Methods section:pcDNA3.1(+)IRES GFP was a gift from Kathleen_L Collins (Addgene plasmid # 51406 ; http://n2t.net/addgene:51406 ; RRID:Addgene_51406)
For your References section:A novel trafficking signal within the HLA-C cytoplasmic tail allows regulated expression upon differentiation of macrophages. Schaefer MR, Williams M, Kulpa DA, Blakely PK, Yaffee AQ, Collins KL. J Immunol. 2008 Jun 15;180(12):7804-17. PubMed 18523244