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(Plasmid #45567)


Item Catalog # Description Quantity Price (USD)
Plasmid 45567 Standard format: Plasmid sent in bacteria as agar stab 1 $85
Cloning Grade DNA 2 µg of cloning grade DNA in Tris buffer 1 $105

This material is available to academics and nonprofits only.


  • Vector backbone
  • Backbone manufacturer
  • Backbone size (bp) 5729
  • Modifications to backbone
    NheI, XhoI, and SacI cloning sites and an encephalomyocarditis virus IRES inserted upstream of EGFP. Puromycin resistance protein (puromycin N-acetyl transferase) fused downstream of EGFP
  • Vector type
    Mammalian Expression
  • Promoter Phcmv (strong human cytomegalovirus immediate early promoter)
  • Selectable markers
    Puromycin ; EGFP
  • Tag / Fusion Protein
    • IRES-EGFP-Puro (C terminal on backbone)

Growth in Bacteria

  • Bacterial Resistance(s)
    Ampicillin, 100 μg/mL
  • Growth Temperature
  • Growth Strain(s)
  • Copy number
    High Copy

Cloning Information

  • Cloning method Restriction Enzyme
  • 5′ sequencing primer CMV-F
  • 3′ sequencing primer pCDH-rev (GCATTCCTTTGGCGAGAG)
  • (Common Sequencing Primers)

Resource Information

Terms and Licenses

  • Zeocin® is an InvivoGen trademark.

Depositor Comments

Plasmid pIRES-EGFP-puro was constructed containing the Phcmv promoter followed by unique restriction sites (NheI, XhoI, and SacI) for insertion of genes. Downstream, an IRES from the encephalomyocarditis virus directs the translation of EGFP-puro from the same mRNA.

Addgene's sequencing results found differences compared to the depositing lab's sequence in the 3' region of the puro resistance marker. The resulting protein sequence matches NCBI WP_055528321.1 and should be functional.

Information for Cloning Grade DNA (Catalog # ( Back to top )


Cloning grade DNA is suitable for use in PCR, cloning reactions, or transformation into E. coli. The purity and amount is not suitable for direct transfections.


  • Amount 2 µg
  • Guaranteed Concentration 100 ng/µl +/- 5 ng/µl
  • Pricing $105 USD
  • Storage DNA can be stored at 4℃ (short term) or -20℃ (long term).

Terms and Licenses

Quality Control

Addgene has verified this plasmid using Next Generation Sequencing. Results are available here

How to cite this plasmid ( Back to top)

These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.

  • For your Materials & Methods section:

    pIRES-EGFP-puro was a gift from Michael McVoy (Addgene plasmid # 45567 ; ; RRID:Addgene_45567)