181,758 results
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Plasmid#89567PurposePlasmid expression dCas9 protein in fusion with KRAB domainDepositorInsertdCas9
UseLentiviralTagsBlasticidin resistance gene and KRABMutationD10A and H840AAvailable sinceJune 30, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
mCherry-Lifeact-7
Plasmid#54491PurposeLocalization: Actin, Excitation: 587, Emission: 610DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertmCherry-Lifeact
ExpressionMammalianAvailable sinceJune 20, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pcDNA3-HA-Sumo1
Plasmid#21154PurposeMammalian expression of human Sumo1 with HA tagDepositorAvailable sinceMay 11, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
HA-HIF2alpha-pcDNA3
Plasmid#18950DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertHypoxia inducible factor 2 alpha (EPAS1 Human)
TagsHA-tagExpressionMammalianMutationwt cDNAAvailable sinceAug. 20, 2008AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pIRESneo-FLAG/HA Ago2 corrected
Plasmid#10822DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertArgonaute 2 (AGO2 Human)
TagsFLAG and HAExpressionMammalianMutation3 silent mutations in orfAvailable sinceNov. 2, 2005AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pLVX-TetOne-Puro-GFP
Plasmid#171123PurposeDoxycycline inducible expression of GFPDepositorInsertEGFP
UseLentiviralExpressionMammalianPromoterTRE3GSAvailable sinceSept. 30, 2022AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAAV-hSyn-Cre-P2A-dTomato (AAV Retrograde)
Viral prep#107738-AAVrgPurposeReady-to-use AAV Retrograde particles produced from pAAV-hSyn-Cre-P2A-dTomato (#107738). In addition to the viral particles, you will also receive purified pAAV-hSyn-Cre-P2A-dTomato plasmid DNA. hSyn-driven expression of Cre and dTomato (physically separate). These AAV were produced with a retrograde serotype, which permits retrograde access to projection neurons. These AAV preparations are suitable purity for injection into animals.DepositorPromoterSynTagsdTomato (physically separate, not a fusion protein)Available sinceJune 15, 2020AvailabilityAcademic Institutions and Nonprofits only -
pAAV-hSyn-EGFP
Plasmid#50465PurposeEGFP under the control of human synapsin promoterDepositorHas serviceAAV1, AAV1 trial size, AAV2, AAV2 trial size, AAV5, AAV5 trial size, AAV8, AAV8 trial size, AAV9, AAV9 trial size, AAV11, AAV11 trial size, AAV PHP.eB, AAV Retrograde, and AAV Retrograde trial sizeInsertEGFP
UseAAVTagsN/APromoterhuman Synapsin 1Available sinceMarch 17, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
AAV-GFP/Cre
Plasmid#49056PurposeAdeno-associated virus (AAV) encoding a green fluorescent protein/Cre recombinase (GFP/Cre) fusion protein.DepositorInsertCre
UseAAV, Cre/Lox, and Mouse TargetingTagsEGFP, Myc, and NLSExpressionMammalianPromoterCMVAvailable sinceFeb. 13, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pSQT1601
Plasmid#53369Purposehuman codon optimized Csy4 and FokI-dCas9 expression plasmidDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInserthCsy4-T2A-NLS-hFokI-dCas9-NLS
UseCRISPRExpressionMammalianPromoterCAGAvailable sinceJune 19, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pGL3 BRE Luciferase
Plasmid#45126DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertId1-BRE
UseLuciferase; Bmp/smad transcriptional reporterExpressionMammalianAvailable sinceJan. 3, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
GFP-Nucleolin
Plasmid#28176DepositorPublicationHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertNucleolin (NCL Human)
TagsGFPExpressionMammalianAvailable sinceFeb. 24, 2011AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pMRX-IP-GFP-LC3-RFP
Plasmid#84573PurposeExpresses GFP-LC3-RFP in mammalian cells to measure autophagic fluxDepositorInsertmicrotubule-associated protein 1 light chain 3 beta (Map1lc3b Rat)
UseRetroviralTagsEGFP and mRFP1ExpressionMammalianAvailable sinceJan. 20, 2017AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pAC154-dual-dCas9VP160-sgExpression
Plasmid#48240PurposeDual expression construct expressing both dCas9VP160 and sgRNA from separate promotersDepositorInsertdCas9
UseCRISPRTagsHA-Tag, HA-tag, and VP160ExpressionMammalianMutationD10A H840AAvailable sinceSept. 27, 2013AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pENTR1A no ccDB (w48-1)
Plasmid#17398PurposeGateway entry 1A vector, no ccDB geneDepositorPublicationTypeEmpty backboneUseGateway CloningExpressionMammalianAvailable sinceAug. 12, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by -
LSL-Cas9-Rosa26TV
Plasmid#61408PurposeCre-dependent Cas9 expression plasmid and targeting vector for the mouse Rosa26 locus. Cas9 expression is Cre-dependent and the targeting vector has positive (Neo) and negative (DTA) selection.DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertsCas9
EGFP
UseCRISPR, Cre/Lox, and Mouse TargetingTags3xFLAG and P2AExpressionMammalianMutationhuman codon optimizedPromoterCAGAvailable sinceJan. 15, 2015AvailabilityAcademic Institutions and Nonprofits onlyCited by -
pEBFP2-Nuc
Plasmid#14893PurposeMammalian expression of EBFP2 blue fluorescent protein with NLSDepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertpEBFP2-Nuc
TagsNLSExpressionMammalianMutationReplace EYFP of the vector pEYFP-Nuc from Clontec…Available sinceMay 15, 2007AvailabilityAcademic Institutions and Nonprofits onlyCited by -
NFAT luciferase reporter
Plasmid#10959DepositorHas service50 µg Transfection-Ready DNA and 100 µg Transfection-Ready DNAInsertIL-2 promoter (IL2 Human)
UseLuciferaseTagsluciferaseExpressionMammalianAvailable sinceJan. 6, 2006AvailabilityAcademic Institutions and Nonprofits onlyCited by -
FH-TET1-pEF
Plasmid#49792PurposeThis plasmid expresses the full-length human TET1 gene with a Flag and HA tag.DepositorInsertTet methylcytosine dioxygenase 1 (TET1 Human)
TagsFlag and HAExpressionMammalianPromoterEF-1alphaAvailable sinceJan. 22, 2014AvailabilityAcademic Institutions and Nonprofits onlyCited by -
MSCV-XZ066-EGFRvIII
Plasmid#20737DepositorPublicationInsertEGFRvIII (EGFR Human)
UseRetroviralExpressionMammalianMutationEGFR with a 267 amino acid deletion in the extrac…Available sinceMarch 31, 2009AvailabilityAcademic Institutions and Nonprofits onlyCited by