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PurposeDoxycycline inducible expression of GFP
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Depositing Lab
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Sequence Information
Ordering
| Item | Catalog # | Description | Quantity | Price (USD) | |
|---|---|---|---|---|---|
| Plasmid | 171123 | Standard format: Plasmid sent in bacteria as agar stab | 1 | $85 | |
This material is available to academics and nonprofits only.
Backbone
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Vector backbonepLVX-TetOne-Puro
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Backbone manufacturerClontech
- Backbone size w/o insert (bp) 9173
- Total vector size (bp) 9894
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Vector typeMammalian Expression, Lentiviral
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Selectable markersPuromycin
Growth in Bacteria
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Bacterial Resistance(s)Ampicillin, 100 μg/mL
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Growth Temperature37°C
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Growth Strain(s)NEB Stable
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Copy numberUnknown
Gene/Insert
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Gene/Insert nameEGFP
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Insert Size (bp)720
Resource Information
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Supplemental Documents
Terms and Licenses
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Academic/Nonprofit Terms
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Industry Terms
- Not Available to Industry
Trademarks:
- Zeocin® is an InvivoGen trademark.
Depositor Comments
pLVX-TetOne-Puro backbone from pLVX-TetOne-Puro-hAXL (Plasmid #124797)
EGFP insert from Lenti-Cas9-gRNA-GFP (Addgene #124770)
How to cite this plasmid
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These plasmids were created by your colleagues. Please acknowledge the Principal Investigator, cite the article in which the plasmids were described, and include Addgene in the Materials and Methods of your future publications.
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For your Materials & Methods section:
pLVX-TetOne-Puro-GFP was a gift from Jason Sheltzer (Addgene plasmid # 171123 ; http://n2t.net/addgene:171123 ; RRID:Addgene_171123) -
For your References section:
Chromosomal instability accelerates the evolution of resistance to anti-cancer therapies. Lukow DA, Sausville EL, Suri P, Chunduri NK, Wieland A, Leu J, Smith JC, Girish V, Kumar AA, Kendall J, Wang Z, Storchova Z, Sheltzer JM. Dev Cell. 2021 Sep 13;56(17):2427-2439.e4. doi: 10.1016/j.devcel.2021.07.009. Epub 2021 Aug 4. 10.1016/j.devcel.2021.07.009 PubMed 34352222
Map uploaded by the depositor.
