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pAC154-dual-dCas9VP160-sgExpression Citations (18)

Originally described in: Multiplexed activation of endogenous genes by CRISPR-on, an RNA-guided transcriptional activator system.
Cheng AW, Wang H, Yang H, Shi L, Katz Y, Theunissen TW, Rangarajan S, Shivalila CS, Dadon DB, Jaenisch R Cell Res. 2013 Aug 27. doi: 10.1038/cr.2013.122.
PubMed Journal

Articles Citing pAC154-dual-dCas9VP160-sgExpression

Human Induced Pluripotent Stem Cell NEUROG2 Dual Knockin Reporter Lines Generated by the CRISPR/Cas9 System. Li S, Xue H, Wu J, Rao MS, Kim DH, Deng W, Liu Y. Stem Cells Dev. 2015 Dec 15;24(24):2925-42. doi: 10.1089/scd.2015.0131. Epub 2015 Nov 5. PubMed
Potent and Targeted Activation of Latent HIV-1 Using the CRISPR/dCas9 Activator Complex. Saayman SM, Lazar DC, Scott TA, Hart JR, Takahashi M, Burnett JC, Planelles V, Morris KV, Weinberg MS. Mol Ther. 2015 Nov 19. doi: 10.1038/mt.2015.202. PubMed
Spell Checking Nature: Versatility of CRISPR/Cas9 for Developing Treatments for Inherited Disorders. Wojtal D, Kemaladewi DU, Malam Z, Abdullah S, Wong TW, Hyatt E, Baghestani Z, Pereira S, Stavropoulos J, Mouly V, Mamchaoui K, Muntoni F, Voit T, Gonorazky HD, Dowling JJ, Wilson MD, Mendoza-Londono R, Ivakine EA, Cohn RD. Am J Hum Genet. 2016 Jan 7;98(1):90-101. doi: 10.1016/j.ajhg.2015.11.012. Epub 2015 Dec 10. PubMed
Generation and Characterization of a MYF5 Reporter Human iPS Cell Line Using CRISPR/Cas9 Mediated Homologous Recombination. Wu J, Hunt SD, Xue H, Liu Y, Darabi R. Sci Rep. 2016 Jan 5;6:18759. doi: 10.1038/srep18759. PubMed
Engineering Synthetic Gene Circuits in Living Cells with CRISPR Technology. Jusiak B, Cleto S, Perez-Pinera P, Lu TK. Trends Biotechnol. 2016 Jan 22. pii: S0167-7799(15)00274-7. doi: 10.1016/j.tibtech.2015.12.014. PubMed
Precision Modulation of Neurodegenerative Disease-Related Gene Expression in Human iPSC-Derived Neurons. Heman-Ackah SM, Bassett AR, Wood MJ. Sci Rep. 2016 Jun 24;6:28420. doi: 10.1038/srep28420. PubMed
Inheritable Silencing of Endogenous Genes by Hit-and-Run Targeted Epigenetic Editing. Amabile A, Migliara A, Capasso P, Biffi M, Cittaro D, Naldini L, Lombardo A. Cell. 2016 Sep 22;167(1):219-232.e14. doi: 10.1016/j.cell.2016.09.006. PubMed
Increased Expression of Laminin Subunit Alpha 1 Chain by dCas9-VP160. Perrin A, Rousseau J, Tremblay JP. Mol Ther Nucleic Acids. 2017 Mar 17;6:68-79. doi: 10.1016/j.omtn.2016.11.004. Epub 2016 Dec 10. PubMed
Large, Diverse Population Cohorts of hiPSCs and Derived Hepatocyte-like Cells Reveal Functional Genetic Variation at Blood Lipid-Associated Loci. Pashos EE, Park Y, Wang X, Raghavan A, Yang W, Abbey D, Peters DT, Arbelaez J, Hernandez M, Kuperwasser N, Li W, Lian Z, Liu Y, Lv W, Lytle-Gabbin SL, Marchadier DH, Rogov P, Shi J, Slovik KJ, Stylianou IM, Wang L, Yan R, Zhang X, Kathiresan S, Duncan SA, Mikkelsen TS, Morrisey EE, Rader DJ, Brown CD, Musunuru K. Cell Stem Cell. 2017 Apr 6;20(4):558-570.e10. doi: 10.1016/j.stem.2017.03.017. PubMed
Mutant Cas9-transcriptional activator activates HIV-1 in U1 cells in the presence and absence of LTR-specific guide RNAs. Kim V, Mears BM, Powell BH, Witwer KW. Matters (Zur). 2017;2017. doi: 10.19185/matters.201611000027. Epub 2017 Jan 12. PubMed
Systematic analysis of transcription start sites in avian development. Lizio M, Deviatiiarov R, Nagai H, Galan L, Arner E, Itoh M, Lassmann T, Kasukawa T, Hasegawa A, Ros MA, Hayashizaki Y, Carninci P, Forrest ARR, Kawaji H, Gusev O, Sheng G. PLoS Biol. 2017 Sep 5;15(9):e2002887. doi: 10.1371/journal.pbio.2002887. eCollection 2017 Sep. PubMed
A cautionary tale of sense-antisense gene pairs: independent regulation despite inverse correlation of expression. Goyal A, Fiskin E, Gutschner T, Polycarpou-Schwarz M, Gross M, Neugebauer J, Gandhi M, Caudron-Herger M, Benes V, Diederichs S. Nucleic Acids Res. 2017 Dec 1;45(21):12496-12508. doi: 10.1093/nar/gkx952. PubMed
DNA methylation dynamics during embryonic development and postnatal maturation of the mouse auditory sensory epithelium. Yizhar-Barnea O, Valensisi C, Jayavelu ND, Kishore K, Andrus C, Koffler-Brill T, Ushakov K, Perl K, Noy Y, Bhonker Y, Pelizzola M, Hawkins RD, Avraham KB. Sci Rep. 2018 Nov 26;8(1):17348. doi: 10.1038/s41598-018-35587-x. PubMed
Identification of on-target mutagenesis during correction of a beta-thalassemia splice mutation in iPS cells with optimised CRISPR/Cas9-double nickase reveals potential safety concerns. Alateeq S, Ovchinnikov D, Tracey T, Whitworth D, Al-Rubaish A, Al-Ali A, Wolvetang E. APL Bioeng. 2018 Dec 3;2(4):046103. doi: 10.1063/1.5048625. eCollection 2018 Dec. PubMed
Targeted Transgene Activation in the Brain Tissue by Systemic Delivery of Engineered AAV1 Expressing CRISPRa. Lau CH, Ho JW, Lo PK, Tin C. Mol Ther Nucleic Acids. 2019 Jun 7;16:637-649. doi: 10.1016/j.omtn.2019.04.015. Epub 2019 Apr 23. PubMed

Associated Plasmids

Functional genetic variants can mediate their regulatory effects through alteration of transcription factor binding. Johnston AD, Simoes-Pires CA, Thompson TV, Suzuki M, Greally JM. Nat Commun. 2019 Aug 2;10(1):3472. doi: 10.1038/s41467-019-11412-5. PubMed
Off-target effects of CRISPRa on interleukin-6 expression. Soubeyrand S, Lau P, Peters V, McPherson R. PLoS One. 2019 Oct 28;14(10):e0224113. doi: 10.1371/journal.pone.0224113. eCollection 2019. PubMed
NPAS4L is involved in avian hemangioblast specification. Weng W, Nagai H, Hamidi S, Sheng G. Haematologica. 2020 Nov 1;105(11):2647-2650. doi: 10.3324/haematol.2019.239434. PubMed

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