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An Autophagic Flux Probe that Releases an Internal Control.
Kaizuka T, Morishita H, Hama Y, Tsukamoto S, Matsui T, Toyota Y, Kodama A, Ishihara T, Mizushima T, Mizushima N
Mol Cell. 2016 Nov 17;64(4):835-849. doi: 10.1016/j.molcel.2016.09.037. Epub 2016 Nov 3. PubMed Article

Plasmids from Article

ID Plasmid Purpose
84572pMRX-IP-GFP-LC3-RFP-LC3ΔGExpresses GFP-LC3-RFP-LC3ΔG in mammalian cells to measure autophagic flux
84573pMRX-IP-GFP-LC3-RFPExpresses GFP-LC3-RFP in mammalian cells to measure autophagic flux
168997pcDNA3-GFP-LC3-RFP-LC3ΔGExpresses GFP-LC3-RFP-LC3ΔG in mammalian cells and zebrafish to measure autophagic flux
168998pT2AL200R150G-EGFP-LC3B-RFP-LC3BΔGExpresses GFP-LC3-RFP-LC3ΔG in zebrafish to measure autophagic flux

Antibodies from Article