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pHAGE-EGFR__ERP44

Plasmid

#116815

Purpose

Lentiviral expression of EGFR__ERP44 fusion

Depositor

Gordon Mills, Kenneth Scott

Article

Ng et al Cancer Cell. 2018 Mar 12;33(3):450-462.e

Insert

EGFR__ERP44

Use

Lentiviral

Available Since

March 14, 2019

Availability

Academic Institutions and Nonprofits only

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TRE3GV-SpdCas9-Dnmt3A/3L-WT

Plasmid

#222502

Purpose

Expresses TRE3GV promoter driven human codon optimized SpdCas9 directly fused to WT Dnmt3A/3L and hPGK promoter driven mCherry

Depositor

Isaac Hilton

Article

Guerra-Resendez et al ACS Synth Biol. 2025 Feb 3. doi: 10.1021

Inserts

S. pyogenes dCas9 with C-terminal fusion of murine Dnmt3A-Dnmt3L engineered fusion
mCherry

Use

Lentiviral

Tags

FLAG Tag

Expression

Mammalian

Mutation

D10A and H840A in S.pyogenes Cas9

Promoter

TRE3GV and hPGK

Available Since

Feb. 7, 2025

Availability

Academic Institutions and Nonprofits only

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pLC-RFP657-CASP8

Plasmid

#75164

Purpose

LentiCRISPR-RFP657 with sgRNA targeting human Caspase-8

Depositor

Beat Bornhauser

Article

McComb et al Sci Transl Med. 2016 May 18;8(339):339ra

Insert

CASP8 sgRNA (CASP8 Human)

Use

CRISPR and Lentiviral

Expression

Mammalian

Promoter

U6

Available Since

June 8, 2016

Availability

Academic Institutions and Nonprofits only

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Myc tag S16-A PTB

Plasmid

#23025

Depositor

Douglas Black

Article

Xie et al Proc Natl Acad Sci U S A. 2003 Jul 22. 1

Insert

PTB1 (PTBP1 Human)

Tags

Myc

Expression

Mammalian

Mutation

Changed Serine 16 to Alanine

Available Since

June 4, 2010

Availability

Academic Institutions and Nonprofits only
pET51b_SNAP-ecDHFR

Plasmid

#187108

Purpose

Expression of SNAP-ecDHFR fusion in bacteria

Depositor

Kai Johnsson

Article

Wang et al Angew Chem Int Ed Engl. 2020 Dec 1;59(49

Insert

SNAP-ecDHFR

Tags

Hisx10 and Strep

Expression

Bacterial

Promoter

T7

Available Since

July 20, 2022

Availability

Academic Institutions and Nonprofits only

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pDropIn-T2A-split-intein-Gal4[1-20]-N-int

Plasmid

#199195

Purpose

Insert to create split-intein Gal4[N-int] knock-in vectors via Drop-In cloning, released via BsmBI digest

Depositor

Norbert Perrimon

Article

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

T2A-split-intein-Gal4[1-20]-N-int, 3xP3-dsRed

Expression

Insect

Available Since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pDropIn-T2A-split-intein-Gal4[21-881]-C-int

Plasmid

#199196

Purpose

Insert to create split-intein Gal4[C-int] knock-in vectors via Drop-In cloning, released via BsmBI digest

Depositor

Norbert Perrimon

Article

Ewen-Campen et al Proc Natl Acad Sci U S A. 2023 Jun 13;12

Insert

T2A-split-intein-Gal4[21-881]-C-int, 3xP3-dsRed

Expression

Insect

Available Since

Aug. 30, 2023

Availability

Academic Institutions and Nonprofits only

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pLJM60-Tiar

Plasmid

#38244

Depositor

David Sabatini

Article

Thoreen et al Nature. 2012 May 2;485(7396):109-13. doi

Insert

Tiar (Tial1 Mouse)

Use

Lentiviral

Tags

FLAG

Expression

Mammalian

Promoter

CMV

Available Since

Nov. 7, 2012

Availability

Academic Institutions and Nonprofits only
px458_2A_GFP_sgRNA_G3BP2_G1

Plasmid

#127112

Depositor

Thomas Tuschl

Article

Meyer et al Mol Cell. 2020 Mar 19;77(6):1193-1205.e5

Insert

gRNA G3BP2 (G3BP2 Human)

Use

CRISPR

Available Since

Aug. 9, 2019

Availability

Academic Institutions and Nonprofits only

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LITE1.0_pAAV_hSyn_CRY2PHR-NLS-SID4X_2A_phiLOV2.1_WPRE_bGHpA

Plasmid

#47454

Purpose

epiLITE / LITE1.0 CRY2PHR fused to SID4X transcriptional repressor effector domain. Binds to CIB1 upon blue light stimulation. Synapsin promoter for neuronal expression.

Depositor

Feng Zhang

Article

Konermann et al Nature. 2013 Jul 23. doi: 10.1038/nature

Insert

CRY2PHR-NLS-SID4X

Use

AAV

Tags

2A_phiLOV2.1

Expression

Mammalian

Promoter

hSyn human synapsin (mammalian neuronal expressio…

Available Since

Oct. 15, 2013

Availability

Academic Institutions and Nonprofits only

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AiP12229 - pAAV-AiE0441h-minBG-SYFP2-WPRE3-BGHpA (Alias: CN2229)

Plasmid

#191718

Purpose

AI plasmid ALIAS: AiP12229. Expression of SYFP2 (yellow fluorescent protein) in striatal medium spiny neurons

Depositor

Allen Institute for Brain Science, Jonathan Ting

Article

Hunker et al Neuron. 2025 May 21;113(10):1507-1524.e1

Insert

SYFP2

Use

AAV

Mutation

none

Available Since

Feb. 1, 2024

Availability

Academic Institutions and Nonprofits only

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B6-1

Plasmid

#111485

Purpose

Phe-tRNA synthetase B

Depositor

Cheemeng Tan

Article

Villarreal et al Nat Chem Biol. 2018 Jan;14(1):29-35. doi

Insert

Phe-tRNA synthetase B

Tags

6xHis

Expression

Bacterial

Available Since

Sept. 14, 2018

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1(+) M-Ras(WT)

Plasmid

#224287

Purpose

Cell transfection and expression of M-Ras

Depositor

Kevan Shokat

Article

Morstein et al Cell. 2024 Sep 6:S0092-8674(24)00908-5.

Insert

M-Ras (MRAS Human)

Expression

Mammalian

Available Since

Sept. 18, 2024

Availability

Academic Institutions and Nonprofits only

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pcDNA3.1-P2X2-CFP

Plasmid

#22401

Depositor

Henry Lester

Article

Khakh et al J Neurosci. 2005 Jul 20. 25(29):6911-20.

Insert

ATP-gated P2X channel 2 (P2rx2 Rat)

Tags

CFP

Expression

Mammalian

Available Since

Dec. 10, 2009

Availability

Academic Institutions and Nonprofits only
pCaSpeR4 mito roGFP2-Orp1

Plasmid

#65001

Purpose

Expression of mitochondrial roGFP2-Orp1 under the alpha Tub84B promoter

Depositor

Tobias Dick

Article

Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

Insert

Orp1 (HYR1 Budding Yeast)

Tags

roGFP2

Expression

Insect

Mutation

mitochondrial targeting sequence

Available Since

Nov. 2, 2015

Availability

Academic Institutions and Nonprofits only

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pCaSper4 cyto roGFP2-Orp1

Plasmid

#64998

Purpose

Expression of cytosolic roGFP2-Orp1 under the alphaTub84B promoter

Depositor

Tobias Dick

Article

Albrecht et al Cell Metab. 2011 Dec 7;14(6):819-29. doi

Insert

Orp1 (HYR1 Budding Yeast)

Tags

roGFP2

Expression

Insect

Available Since

July 8, 2015

Availability

Academic Institutions and Nonprofits only

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iLOV-Lifeact-7

Plasmid

#54746

Purpose

Localization: Actin, Excitation: 450, Emission: 495

Depositor

Michael Davidson

Insert

Lifeact-iLOV

Expression

Mammalian

Available Since

July 25, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pLKO.1-OSBP

Plasmid

#32495

Depositor

Alex Toker

Article

Nhek et al Mol Biol Cell. 2010 Jul 1;21(13):2327-37

Insert

OSBP (OSBP Human)

Use

Lentiviral and RNAi

Expression

Mammalian

Promoter

U6

Available Since

Sept. 26, 2011

Availability

Academic Institutions and Nonprofits only
iLOV-C1

Plasmid

#54591

Purpose

Localization: C1 Cloning Vector, Excitation: 450, Emission: 495

Depositor

Michael Davidson

Type

Empty backbone

Tags

iLOV

Expression

Mammalian

Available Since

June 12, 2014

Availability

Industry, Academic Institutions, and Nonprofits
pC1.530

Plasmid

#205441

Purpose

Level 1 Position 1 pUC19 vector with sgRNA and GmR. Used for homologous recombination with Cas12a editing vector pC1.509. sgRNA targets pC1.509 for removal.

Depositor

Alistair McCormick

Article

Victoria et al Plant Physiol. 2024 May 7:kiae261. doi:

Insert

RepB gRNA

Use

Synthetic Biology

Available Since

May 10, 2024

Availability

Academic Institutions and Nonprofits only

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