-
Plasmid#54301PurposeLocalization: Intermediate Filaments, Excitation: 487, Emission: 509DepositorInsertVimentin (VIM Human)
UseTagsmEmeraldExpressionMammalianMutationPromoterCMVAvailable sinceJune 16, 2014AvailabilityIndustry, Academic Institutions, and Nonprofits -
pLX304-MARK3
Plasmid#107235PurposeLentiviral gene expression vector for phospho-MARK3 expressionDepositorInsertMARK3
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 20, 2018AvailabilityAcademic Institutions and Nonprofits only -
pYPQ269E2
Plasmid#164721PurposeGateway entry clone (attL1 & attR5) A3A/Y130F-CBE_V04 for C-T base editingDepositorInsertA3A(Y130F)-zCas9(D10A)-UGI-T2A-MS2-UGI
UseCRISPR; Gateway compatible a3a/y130f-cbe_v04 entr…TagsExpressionPlantMutationPromoterAvailable sinceMay 21, 2021AvailabilityAcademic Institutions and Nonprofits only -
Bmi1 shRNA1
Plasmid#21576DepositorInsertBmi-1 (Bmi1 Mouse)
UseLentiviralTagsExpressionMammalianMutationPromoterAvailable sinceJune 2, 2010AvailabilityAcademic Institutions and Nonprofits only -
TFORF2462
Plasmid#142092PurposeLentiviral vector for overexpressing transcription factor ORFs with unique 24-bp barcodes. Barcodes facilitate identification of transcription factors in pooled screens.DepositorInsertIRF7 (IRF7 Human)
UseLentiviralTagsExpressionMammalianMutationPromoterEF1aAvailable sinceJune 8, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJMP12042
Plasmid#222354PurposeTn7 integrative vector with PabstBR promoter expression cassette and aprR marker on Tn7 transposon; empty vector plasmid with MCS for cloning - we recommend NcoI/BamHI.DepositorTypeEmpty backboneUseTagsExpressionBacterialMutationPromoterAvailable sinceJuly 15, 2024AvailabilityAcademic Institutions and Nonprofits only -
pENTR221-YAP1 CA
Plasmid#79502PurposeMultiSite Gateway entry clones, second fragment (attL5, attL2) Human YAP1 S127A mutant CDSDepositorInsertYAP1 (YAP1 Human)
UseMultisite gateway entry cloneTags3XFLAGExpressionMutationS127APromoterno PromoterAvailable sinceMay 10, 2017AvailabilityAcademic Institutions and Nonprofits only -
pDSG288
Plasmid#115607Purposesurface-displayed Ag3, used in composability experiments; low copyDepositorInsertregulated adhesin construct
UseTagsExpressionBacterialMutationPromoterAvailable sinceOct. 11, 2018AvailabilityAcademic Institutions and Nonprofits only -
pGM202T7
Plasmid#69993PurposeE. coli/Streptomyces expression vector containing the T7-promoterDepositorInsertsaphII
tsr
rep
sso
T7 promoter
dso
His-Orf
UseTagsN-terminal and C-terminal His tag encoding sequen…ExpressionBacterialMutationPromoterAvailable sinceNov. 13, 2015AvailabilityAcademic Institutions and Nonprofits only -
pNos-PE2-attB
Plasmid#149549PurposeExpression of PE2 enzyme under control of the germ cell specific nanos promoter. Can be used to generate transgenic flies with vermillion+ selection.DepositorInsertPE2
UseCRISPRTagsExpressionInsectMutationPromoternanos (nos)Available sinceAug. 6, 2020AvailabilityAcademic Institutions and Nonprofits only -
CROP_C9_Puro_FGFR3
Plasmid#183291PurposeAll-in-One CRISPRko system with a guide RNA that targets FGFR3 geneDepositorInsertFGFR3
UseLentiviralTagsExpressionMutationPromoterAvailable sinceJune 16, 2022AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-C1 hSlp2-a
Plasmid#40047DepositorInserthSlp2-a (SYTL2 Human)
UseTagsEGFPExpressionMammalianMutationPromoterCMV promoterAvailable sinceOct. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pRSII422
Plasmid#35462DepositorTypeEmpty backboneUseYeast 2 micron vectorTagsExpressionMutationPromoterNoneAvailable sinceOct. 18, 2012AvailabilityAcademic Institutions and Nonprofits only -
pSLIK-NFLAG-hEWSR1G511A-CMyc
Plasmid#50734PurposeProduces lentivirus expressing human EWSR1G511A mutant with FLAG tag at its N-terminus and Myc tag at its C-terminus by co-transfection with psPAX2 and pMD2GDepositorInsertEWSR1 G511A (EWSR1 Human)
UseLentiviralTagsFLAG and MycExpressionMutationChanged Gly 511 to AlaPromoterTREAvailable sinceJan. 16, 2014AvailabilityAcademic Institutions and Nonprofits only -
pEGFP-hsRPA4 (MSW#517)
Plasmid#208070PurposeExpression of GFP-tagged hsRPA4 in human cellsDepositorInsertRPA4 (RPA4 Human)
UseTagsGFPExpressionMammalianMutationPromoterAvailable sinceDec. 11, 2023AvailabilityAcademic Institutions and Nonprofits only -
pMS_366
Plasmid#182131Purposeexpresses retron RNA, ec.86 Reverse Transcriptase, and CspRecT SSAP, to create a specific rifampicin resistance mutationDepositorInsertsretron RNA
ec.86 RT
CspRecT
UseTagsExpressionMutationincorporated rifampicin-resistance-conferring don…PromoterAvailable sinceSept. 1, 2023AvailabilityAcademic Institutions and Nonprofits only -
pJBL711
Plasmid#140381PurposeExpresses OtrR protein under the control of T7-lacO promoterDepositorInsertT7-lacO-OtrR-T
UseTagsTEV-6XHisExpressionBacterialMutationPromoterAvailable sinceJuly 8, 2020AvailabilityAcademic Institutions and Nonprofits only -
pIC291
Plasmid#51403PurposeRetroviral expression of EGFP-TEV-S-tag SKA1 for protein localization and affinity purification (LAP)DepositorInsertSka1 IMAGE 3909951 (SKA1 Human)
UseRetroviralTagsEGFP, S tag, and TEV protease siteExpressionMammalianMutationPromoterAvailable sinceMarch 31, 2014AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPR v2-sgACSL4 clone2
Plasmid#162129PurposeLentiviral sgRNA plasmid targeting human ACSL4DepositorInsertsgACSL4 (ACSL4 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only -
LentiCRISPR v2-sgACSL4 clone1
Plasmid#162128PurposeLentiviral sgRNA plasmid targeting human ACSL4DepositorInsertsgACSL4 (ACSL4 Human)
UseLentiviralTagsExpressionMutationPromoterAvailable sinceFeb. 9, 2021AvailabilityAcademic Institutions and Nonprofits only