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174,828 results

Showing: 701 - 720 of 174828 results

  3. MoClo Yeast Secretion and Display (YSD) Toolkit

    Plasmid Kit
    #1000000240
    Purpose
    Collection of plasmids to optimize secretion and cell surface display of proteins of interest in yeast.
    Depositor
    Paul Young
    Application
    Cloning and Synthetic Biology
    Vector Type
    Yeast Expression
    Cloning Type
    Golden Gate (MoClo)
    Available Since
    April 10, 2024
    Availability
    Academic Institutions and Nonprofits only

  7. EGFP-CAAX

    Plasmid
    #86056
    Purpose
    A mammalian expression plasmid encoding farnesylation motif of HRAS with N-terminus EGFP.
    Depositor
    Lei Lu
    Article
    Madugula et al J Cell Sci. 2016 Oct 15;129(20):3922-393
    Insert
    Homo sapiens Harvey rat sarcoma (HRAS Human)
    Tags
    GFP
    Expression
    Mammalian
    Mutation
    contains the region from 825-857 of HRAS as a far…
    Promoter
    CMV
    Available Since
    May 8, 2017
    Availability
    Academic Institutions and Nonprofits only
    86056-plasmid-map-sequence-id-177924

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  9. pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE (AAV9)

    Viral Prep
    #162380-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE (#162380). In addition to the viral particles, you will also receive purified pGP-AAV-CAG-FLEX-jGCaMP8s-WPRE plasmid DNA. CAG-driven, Cre-dependent expression of calcium sensor GCaMP8s (more sensitive). These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project
    Article
    Zhang et al Janelia Research Campus, Online Resource
    Promoter
    CAG
    Available Since
    June 22, 2021
    Availability
    Academic Institutions and Nonprofits only

  10. pMA3790

    Plasmid
    #70110
    Purpose
    mtDNA elimination/production of rho-0 cells
    Depositor
    Mikhail Alexeyev
    Article
    Spadafora et al PLoS One. 2016 May 2;11(5):e0154684. doi
    Inserts
    EGFP
    hUNG1 Y147A
    Neo
    Tags
    None, human OTC mitochondrial targeting sequence,…
    Expression
    Mammalian
    Mutation
    Y147A, aa's 1-77 removed
    Promoter
    CMV, EF1a, and SV40 and native
    Available Since
    May 3, 2016
    Availability
    Academic Institutions and Nonprofits only
    70110-plasmid-map-sequence-id-399654

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  11. pThermoCas9i_ctrl

    Plasmid
    #100982
    Purpose
    Expresses the catalytically inactive version of ThermoCas9 (Thermo(d)Cas9) and its sgRNA module
    Depositor
    John van der Oost
    Article
    Mougiakos et al Nat Commun. 2017 Nov 21;8(1):1647. doi:
    Inserts
    Catalytically inactive variant of the Cas9 from the type IIc CRISPR-Cas sytem of Geobacillus thermodenitrificans T12 strain
    ThermoCas9 single guide RNA expressing module
    Tags
    None
    Expression
    Bacterial
    Mutation
    changed aspartate 8 to alanine and histidine 582 …
    Promoter
    B. coagulans DSM 1 pta promoter and B. smithii xy…
    Available Since
    Nov. 14, 2017
    Availability
    Academic Institutions and Nonprofits only
    100982-plasmid-map-sequence-id-392326

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  12. mAIRN HO

    Plasmid
    #207411
    Purpose
    Mammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause head-ON transcription-replication conflict (HO TRC).
    Depositor
    Karlene Cimprich
    Article
    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do
    Insert
    mAIRN
    Expression
    Bacterial and Mammalian
    Available Since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    207411-plasmid-map-sequence-id-422696

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  13. mAIRN CD

    Plasmid
    #207412
    Purpose
    Mammalian-cell-based plasmid system that expresses mAIRN (an R-loop-forming transcript) upon dox-induction, which will further cause co-directional transcription-replication conflict (CD TRC).
    Depositor
    Karlene Cimprich
    Article
    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do
    Insert
    mAIRN
    Expression
    Bacterial and Mammalian
    Available Since
    March 27, 2024
    Availability
    Academic Institutions and Nonprofits only
    207412-plasmid-map-sequence-id-422689

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  14. ECFP HO

    Plasmid
    #207413
    Purpose
    Mammalian-cell-based plasmid system that expresses ECFP (a non R-loop-forming transcript) upon dox-induction, which will further cause head-ON transcription-replication conflict (HO TRC).
    Depositor
    Karlene Cimprich
    Article
    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do
    Insert
    ECFP
    Expression
    Bacterial and Mammalian
    Available Since
    Jan. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    207413-plasmid-map-sequence-id-421043

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  15. ECFP CD

    Plasmid
    #207414
    Purpose
    Mammalian-cell-based plasmid system that expresses ECFP (a non R-loop-forming transcript) upon dox-induction, which will further cause co-directional transcription-replication conflict (CD TRC).
    Depositor
    Karlene Cimprich
    Article
    Hamperl et al Cell. 2017 Aug 10;170(4):774-786.e19. do
    Insert
    ECFP
    Expression
    Bacterial and Mammalian
    Available Since
    Jan. 31, 2024
    Availability
    Academic Institutions and Nonprofits only
    207414-plasmid-map-sequence-id-421036

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  17. p37-2iDMD-LR

    Plasmid
    #88892
    Purpose
    37°C full-length DMD luc-red plasmid. Co-expresses human dystrophin, firefly luciferase and mCherry in mammalian cells. Plasmid carries phiC31 and Bxb1 attB sites and can be grown at 37°C.
    Depositor
    Michele Calos
    Article
    Farruggio et al Biotechnol J. 2017 Jan 31. doi: 10.1002/
    Inserts
    Dystrophin (DMD Human)
    luciferase
    mCherry
    Expression
    Mammalian
    Available Since
    May 31, 2017
    Availability
    Academic Institutions and Nonprofits only
    88892-plasmid-map-sequence-id-451853

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  20. pAAV-hSyn-DIO-HA-hM3D(Gq)-IRES-mCitrine (AAV8)

    Viral Prep
    #50454-AAV8
    Purpose
    Ready-to-use AAV8 particles produced from pAAV-hSyn-DIO-HA-hM3D(Gq)-IRES-mCitrine (#50454). In addition to the viral particles, you will also receive purified pAAV-hSyn-DIO-HA-hM3D(Gq)-IRES-mCitrine plasmid DNA. Synapsin-driven, Cre-dependent, excitatory hM3D DREADD expression, with physically separate mCitrine expression for cell body labeling. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    Syn
    Tags
    mCitrine (Cre-dependent)
    Available Since
    Sept. 20, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 701 - 720 of 174828 results