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pGEX-4T-1 3xFlag-CREB

Plasmid

#82721

Purpose

Expresses human CREB

Depositor

Kevin Janes

Article

Shah et al Mol Cell Proteomics. 2017 Apr;16(4 suppl

Insert

CREB (CREB1 Human)

Tags

3XFLAG and GST

Expression

Bacterial

Available Since

Feb. 28, 2019

Availability

Academic Institutions and Nonprofits only

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pDONR223_TBL1XR1_WT

Plasmid

#82059

Purpose

Gateway Donor vector containing TBL1XR1 , part of the Target Accelerator Plasmid Collection.

Depositor

Jesse Boehm, William Hahn, David Root

Article

Kim et al Cancer Discov. 2016 Jul;6(7):714-26. doi

Insert

TBL1XR1 (TBL1XR1 Human)

Use

Gateway entry vector

Mutation

L512P

Promoter

None

Available Since

April 24, 2017

Availability

Academic Institutions and Nonprofits only

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Opto-alpha1AR

Plasmid

#106064

Purpose

Expression of a chimeric putative G-protein coupled receptor (Rhodopsin-alpha1AR; Opto-alpha1AR; F6; 717)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-alpha1AR

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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HaloTag-hCHMP4B Vector

Plasmid

#236972

Purpose

Express HaloTag(R)-hCHMP4B Fusion Protein in Mammalian Cells under a CMV promoter

Depositor

Promega Corporation

Has Service

DNA

Insert

hCHMP4B (CHMP4B Human)

Tags

HaloTag (R)

Expression

Mammalian

Mutation

None

Promoter

CMV

Available Since

July 14, 2025

Availability

Industry, Academic Institutions, and Nonprofits

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pMaster1

Plasmid

#58525

Purpose

Reprogram somatic cells to iPS cells

Depositor

Mario Capecchi

Article

Wu et al Proc Natl Acad Sci U S A. 2014 Jul 22;11

Inserts

neo
tk
oct4 (POU5F1 Human)
Klf4 (KLF4 Human)
Sox2 (SOX2 )
myc (MYC Human)
EBNA-1
Amp
Lin28 (LIN28A Human)
Nanog (NANOG Human)
IRES

Promoter

CAG and EF1a

Available Since

July 5, 2016

Availability

Academic Institutions and Nonprofits only

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Epac-SH225

Plasmid

#191449

Purpose

expression of cAMP sensor in yeast

Depositor

Kees Jalink

Insert

mT2Del-EPACdDEPCD-tdTomato

Expression

Yeast

Available Since

Nov. 11, 2022

Availability

Academic Institutions and Nonprofits only

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pET-MAT2A

Plasmid

#164820

Purpose

Expresses MAT2A in E. coli BL21(DE3)

Depositor

Vern Schramm

Article

Firestone et al J Am Chem Soc. 2017 Oct 4;139(39):13754-

Insert

Methionine Adenosyltransferase 2A (MAT2A Human)

Tags

6x His Tag

Expression

Bacterial

Available Since

Feb. 16, 2021

Availability

Academic Institutions and Nonprofits only

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pSIN-hSNCA-NE

Plasmid

#102366

Purpose

Lentiviral overexpression of synuclein alpha

Depositor

Shu Leong Ho

Article

Ho et al Autophagy. 2019 Apr 14:1-24. doi: 10.108

Insert

synuclein alpha (SNCA Human)

Use

Lentiviral

Tags

NE

Expression

Mammalian

Mutation

A silent mutation (333bp downstream of ATG) was i…

Promoter

EF-1a

Available Since

April 19, 2018

Availability

Academic Institutions and Nonprofits only

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SJZ9-amdS

Plasmid

#228261

Purpose

Template plasmid for yeast gene knockout, allows the use of acetamide as sole nitrogen source

Depositor

Zhiping Xie

Article

Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

Insert

amdS

Expression

Yeast

Promoter

ENO2 S.c.

Available Since

Nov. 21, 2024

Availability

Academic Institutions and Nonprofits only

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V5-bArr2

Plasmid

#201493

Purpose

Expression of the beta-arrestin2 containing a N-terminal V5 tag.

Depositor

Patrick Giguère

Article

Zeghal et al J Biol Chem. 2023 Jul 28:105107. doi: 10

Insert

V5-bArr2 (ARRB2 Human)

Tags

V5

Expression

Mammalian

Promoter

CMV

Available Since

Aug. 10, 2023

Availability

Academic Institutions and Nonprofits only

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pZ:F3-CAGGS GPHTK-F

Plasmid

#112666

Purpose

Genome engineering donor vector for creating master cell lines suitable for FLPe recombinase-mediated cassette exchange (RMCE) in the AAVS1 locus

Depositor

Catherine Verfaillie

Article

Ordovas et al Stem Cell Reports. 2015 Nov 10;5(5):918-

Insert

eGFP

Use

Gene targeting vector for integration at the s1 s…

Expression

Mammalian

Promoter

CAG

Available Since

Nov. 27, 2018

Availability

Academic Institutions and Nonprofits only

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SJZ5-HPH

Plasmid

#228258

Purpose

Template plasmid for yeast gene knockout, confers antibiotic resistance to hygromycin B

Depositor

Zhiping Xie

Article

Feng et al Microbiol Spectr. 2024 Oct 10:e0132024.

Insert

hph

Expression

Yeast

Promoter

SSA1 S.c.

Available Since

Dec. 9, 2024

Availability

Academic Institutions and Nonprofits only

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pLenti-TRE3G-BE3RA-PGK-Puro

Plasmid

#110846

Purpose

Lentiviral vector for dox-inducible expression of BE3RA in mammalian cells (codon optimized)

Depositor

Lukas Dow

Article

Zafra et al Nat Biotechnol. 2018 Jul 3. pii: nbt.419

Insert

BE3RA

Use

Lentiviral

Mutation

D10A

Promoter

TRE3G

Available Since

June 6, 2018

Availability

Academic Institutions and Nonprofits only

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pMSCV-Hygromycin-TTF-1

Plasmid

#119173

Purpose

Retroviral mammalian expression vector containing wt-human TTF-1 cDNA

Depositor

David Mu

Article

Kendall et al Proc Natl Acad Sci U S A. 2007 Oct 16;10

Insert

NKX2-1 (NKX2-1 Human)

Use

Retroviral

Tags

none

Expression

Mammalian

Available Since

Dec. 11, 2018

Availability

Academic Institutions and Nonprofits only

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H2B-GFP

Plasmid

#11680

Depositor

Geoff Wahl

Article

Kanda et al Curr Biol. 1998 Mar 26. 8(7):377-85.

Insert

Human histone H2B (H2BC11 Human)

Tags

EGFP

Expression

Mammalian

Available Since

May 31, 2006

Availability

Academic Institutions and Nonprofits only

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Opto-A2AR

Plasmid

#106066

Purpose

Expression of a chimeric putative G-protein coupled receptor (Rhodopsin-A2AR; Opto-A2AR; F8; 719)

Depositor

Harald Janovjak

Article

Morri et al Nat Commun. 2018 May 16;9(1):1950. doi:

Insert

Opto-A2AR (ADORA2A Bovine, Human)

Tags

Rhodopsin-1D4 and VSV-G

Expression

Mammalian

Mutation

Chimeric receptor protein

Promoter

CMV

Available Since

Jan. 23, 2019

Availability

Academic Institutions and Nonprofits only

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pCMV GATA2

Plasmid

#83820

Purpose

Mammalian expression of mouse Gata2

Depositor

Douglas Engel

Insert

GATA2 (Gata2 Mouse)

Expression

Mammalian

Promoter

CMV

Available Since

Oct. 3, 2016

Availability

Academic Institutions and Nonprofits only
pmSod1-G93A-N’His

Plasmid

#117705

Purpose

mutation on stability/solubility of SOD1

Depositor

Elizabeth Fisher

Insert

Sod1 (Sod1 Mouse)

Tags

His tag with Thrombin cleavage site

Expression

Bacterial

Mutation

G93A

Promoter

T7

Available Since

Nov. 15, 2018

Availability

Academic Institutions and Nonprofits only

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pCMVR8.74

Plasmid

#22036

Purpose

2nd generation lentiviral packaging plasmid. Can be used with 2nd or 3rd generation lentiviral vectors and envelope expressing plasmid (Addgene#12259)

Depositor

Didier Trono

Insert

gag pol tat rev

Expression

Mammalian

Available Since

Oct. 14, 2009

Availability

Academic Institutions and Nonprofits only

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pCB7 alpha5

Plasmid

#16041

Depositor

Filippo Giancotti

Article

Giancotti et al Cell. 1990 Mar 9. 60(5):849-59.

Insert

alpha5 (ITGA5 Human)

Expression

Mammalian

Available Since

Nov. 2, 2007

Availability

Academic Institutions and Nonprofits only