Skip to main content
Addgene
Open Menu

Enter a sequence to perform a BLAST-based search of Addgene plasmid sequence databases.

Information

In Category

Filter By

176,335 results

Showing: 701 - 720 of 176335 results

  1. pCas9D10A_GFP

    Plasmid
    #44720
    Purpose
    Co-expression of human codon-optimized Cas9 (D10A) mutant nickase and GFP, plasmid optimized for expression in human pluripotent stem cells
    Depositor
    Kiran Musunuru
    Article
    Ding et al Cell Stem Cell. 2013 Apr 4;12(4):393-4.
    Insert
    Cas9D10A-2A-GFP
    Use
    CRISPR
    Tags
    2A-GFP
    Expression
    Mammalian
    Mutation
    D10A mutation- nickase mutant
    Promoter
    CAG
    Available Since
    May 8, 2013
    Availability
    Academic Institutions and Nonprofits only
    44720-plasmid-map-sequence-id-179979

    Enlarge

  4. pBABE puro K-Ras V12

    Plasmid
    #9052
    Purpose
    Retroviral expression of a constitutively active form of K-Ras
    Depositor
    William Hahn
    Insert
    KRAS (KRAS Human)
    Use
    Retroviral
    Expression
    Mammalian
    Mutation
    G12V. Constitutively active.
    Available Since
    Nov. 14, 2005
    Availability
    Academic Institutions and Nonprofits only
    9052-plasmid-map-sequence-id-219889

    Enlarge

  5. pAAV-Syn-ChrimsonR-tdT (AAV1)

    Viral Prep
    #59171-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pAAV-Syn-ChrimsonR-tdT (#59171). In addition to the viral particles, you will also receive purified pAAV-Syn-ChrimsonR-tdT plasmid DNA. Syn-driven ChrimsonR-tdTomato expression for optogenetic neural activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Edward Boyden
    Article
    Klapoetke et al Nat Methods. 2014 Mar;11(3):338-46. doi:
    Promoter
    Syn
    Tags
    tdTomato
    Available Since
    Feb. 7, 2018
    Availability
    Academic Institutions and Nonprofits only

  7. pGP-AAV-syn-jGCaMP8f-WPRE (AAV1)

    Viral Prep
    #162376-AAV1
    Purpose
    Ready-to-use AAV1 particles produced from pGP-AAV-syn-jGCaMP8f-WPRE (#162376). In addition to the viral particles, you will also receive purified pGP-AAV-syn-jGCaMP8f-WPRE plasmid DNA. Syn-driven expression of ultrafast calcium sensor GCaMP8f. These AAV preparations are suitable purity for injection into animals.
    Depositor
    GENIE Project
    Article
    Zhang et al Janelia Research Campus, Online Resource
    Promoter
    Syn
    Available Since
    April 26, 2021
    Availability
    Academic Institutions and Nonprofits only

  8. pLentiRNACRISPR_005 - hU6-DR_BsmBI-EFS-RfxCas13d-NLS-2A-Puro-WPRE

    Plasmid
    #138147
    Purpose
    Expresses RfxCas13d in mammalian cells, localized in the nucleus. For cloning of guide RNAs compatible with RfxCas13d. Contains a 5' direct repeat. Clone using BsmBI. F overhang aaac. R overhang aaaa
    Depositor
    Neville Sanjana
    Article
    Wessels et al Nat Biotechnol. 2020 Jun;38(6):722-727.
    Insert
    CasRx
    Use
    Lentiviral
    Tags
    NLS
    Available Since
    Feb. 20, 2020
    Availability
    Academic Institutions and Nonprofits only
    138147-plasmid-map-sequence-id-349848

    Enlarge

  9. pAAV-hSyn-hM3D(Gq)-mCherry (AAV2)

    Viral Prep
    #50474-AAV2
    Purpose
    Ready-to-use AAV2 particles produced from pAAV-hSyn-hM3D(Gq)-mCherry (#50474). In addition to the viral particles, you will also receive purified pAAV-hSyn-hM3D(Gq)-mCherry plasmid DNA. hSyn-driven hM3D(Gq) receptor with an mCherry reporter for CNO-induced neuronal activation. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Bryan Roth
    Promoter
    Syn
    Tags
    mCherry
    Available Since
    Oct. 26, 2016
    Availability
    Academic Institutions and Nonprofits only

  12. pAAV-hSyn-mCherry (AAV9)

    Viral Prep
    #114472-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-hSyn-mCherry (#114472). In addition to the viral particles, you will also receive purified pAAV-hSyn-mCherry plasmid DNA. Synapsin-driven mCherry control vector. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    Syn
    Tags
    mCherry
    Available Since
    Jan. 26, 2021
    Availability
    Academic Institutions and Nonprofits only

  14. mycBioID2-13X Linker-MCS

    Plasmid
    #92308
    Purpose
    To fuse your protein of interest to the C-terminus of BioID2 and use in proximity-dependent biotin identification (BioID); MCS and a 25nm GS linker are present downstream of BioID2 and myc tags.
    Depositor
    Kyle Roux
    Article
    Kim et al Mol Biol Cell. 2016 Feb 24. pii: mbc.E15
    Has Service
    Cloning Grade DNA
    Type
    Empty backbone
    Tags
    Humanized A. aeolicus BioID2 with R40G and Myc
    Expression
    Mammalian
    Promoter
    CMV
    Available Since
    June 29, 2017
    Availability
    Academic Institutions and Nonprofits only
    92308-plasmid-map-sequence-id-216421

    Enlarge

  17. pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA (AAV9)

    Viral Prep
    #20297-AAV9
    Purpose
    Ready-to-use AAV9 particles produced from pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA (#20297). In addition to the viral particles, you will also receive purified pAAV-EF1a-double floxed-hChR2(H134R)-mCherry-WPRE-HGHpA plasmid DNA. Humanized channelrhodopsin H134R mutant fused to mCherry, driven by the EF1a promoter. Cre-dependent. These AAV preparations are suitable purity for injection into animals.
    Depositor
    Karl Deisseroth
    Promoter
    EF1a
    Tags
    mCherry (Cre-dependent)
    Available Since
    March 15, 2018
    Availability
    Academic Institutions and Nonprofits only
Showing: 701 - 720 of 176335 results